Amplification and Sequencing of Mitochondrial DNA in Forensic Casework

2003 ◽  
pp. 213-224 ◽  
Author(s):  
Robert J. Steighner ◽  
Mitchell Holland
Keyword(s):  
Mitochondrial Dna ◽  
Forensic Casework

Cytochrome b and HVI sequences of mitochondrial DNA to identify domestic animal hair in forensic casework

2003 ◽  
Vol 1239 ◽  
pp. 841-845 ◽  
Author(s):  
M.M de Pancorbo ◽  
A Castro ◽  
I Fernández-Fernández ◽  
M.C González-Fernández ◽  
C Martı́nez-Bouzas ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
Domestic Animal ◽  
Forensic Casework ◽  
Animal Hair

Validation of mitochondrial DNA minisequencing for forensic casework

1999 ◽  
Vol 112 (4) ◽  
pp. 241-248 ◽  
Author(s):  
J. M. Morley ◽  
J. E. Bark ◽  
C. E. Evans ◽  
J. G. Perry ◽  
C. A. Hewitt ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Forensic Casework

scholarly journals Recovery of DNA from Footprints in the Snow

2007 ◽  
Vol 121 (3) ◽  
pp. 321 ◽  
Author(s):  
Love Dalén ◽  
Anders Götherström ◽  
Tomas Meijer ◽  
Beth Shapiro
Keyword(s):  
Mitochondrial Dna ◽  
Conservation Genetics ◽  
Conservation Biology ◽  
Genetic Information ◽  
Biological Fluids ◽  
Genetic Data ◽  
Arctic Fox ◽  
Alopex Lagopus ◽  
Widespread Occurrence ◽  
Forensic Casework

The recovery of trace amounts of DNA has been demonstrated to be a reliable tool in conservation genetics and has become a key component of modern forensic casework. To date, genetic data have been successfully recovered from a variety of sources, including biological fluids, faeces, clothing, and even directly from fingerprints. However, to our knowledge and despite their widespread occurrence and clear potential as a source of DNA, genetic information has not previously been recovered directly from footprints. Here, we extract and amplify mitochondrial DNA from a snow footprint, <48-hours old, made by a Swedish Arctic Fox (Alopex lagopus). Our results demonstrate that it is possible to recover sufficient DNA from recent footprints to accurately type the source of the print, with implications for conservation biology and forensic science.

Mitochondrial DNA data analysis strategies that inform MPS-based forensic casework implementation

2019 ◽  
Vol 7 (1) ◽  
pp. 389-391
Author(s):  
Cydne Holt ◽  
Paulina Walichiewicz ◽  
Justin Eagles ◽  
Anthony Daulo ◽  
Meghan Didier ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Data Analysis ◽  
Forensic Casework ◽  
Analysis Strategies

Validation of mitochondrial DNA sequencing for forensic casework analysis

1995 ◽  
Vol 108 (2) ◽  
pp. 68-74 ◽  
Author(s):  
Mark R. Wilson ◽  
Joseph A. DiZinno ◽  
Deborah Polanskey ◽  
Jeri Replogle ◽  
Bruce Budowle
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequencing ◽  
Forensic Casework

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

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