Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes

2003 ◽  
pp. 133-142
Author(s):  
Yutaka Kitami ◽  
Kunio Hiwada
Keyword(s):  
Primer Extension ◽  
Primer Extension Analysis ◽  
Transcription Start ◽  
Transcription Start Sites ◽  
Extension Analysis

scholarly journals A muscle-specific intron enhancer required for rescue of indirect flight muscle and jump muscle function regulates Drosophila tropomyosin I gene expression.

1991 ◽  
Vol 11 (4) ◽  
pp. 1901-1911 ◽  
Author(s):  
J R Schultz ◽  
T Tansey ◽  
L Gremke ◽  
R V Storti
Keyword(s):  
Gene Expression ◽  
Flight Muscle ◽  
Primer Extension ◽  
Primer Extension Analysis ◽  
P Element ◽  
Indirect Flight Muscle ◽  
Mrna Levels ◽  
Enhancer Element ◽  
Mutant Phenotypes ◽  
Extension Analysis

The control of expression of the Drosophila melanogaster tropomyosin I (TmI) gene has been investigated by P-element transformation and rescue of the flightless and jumpless TmI mutant strain, Ifm(3)3. To localize cis-acting DNA sequences that control TmI gene expression, Ifm(3)3 flies were transformed with P-element plasmids containing various deletions and rearrangements of the TmI gene. The effects of these mutations on TmI gene expression were studied by analyzing both the extent of rescue of the Ifm(3)3 mutant phenotypes and determining TmI RNA levels in the transformed flies by primer extension analysis. The results of our analysis indicate that a region located within intron 1 of the gene is necessary and sufficient for directing muscle-specific TmI expression in the adult fly. This intron region has characteristics of a muscle regulatory enhancer element that can function in conjunction with the heterologous nonmuscle hsp70 promoter to promote rescue of the mutant phenotypes and to direct expression of an hsp70-Escherichia coli lacZ reporter gene in adult muscle. The enhancer can be subdivided further into two domains of activity based on primer extension analysis of TmI mRNA levels and on the rescue of mutant phenotypes. One of the intron domains is required for expression in the indirect flight muscle of the adult. The function of the second domain is unknown, but it could regulate the level of expression or be required for expression in other muscle.

Primer Extension Analysis of mRNA

2003 ◽  
pp. 195-199
Author(s):  
Maggie Walmsley ◽  
Mark Leonard ◽  
Roger Patient
Keyword(s):  
Primer Extension ◽  
Primer Extension Analysis ◽  
Extension Analysis

scholarly journals Multiplex primer extension analysis for rapid detection of major European mitochondrial haplogroups

2006 ◽  
Vol 27 (19) ◽  
pp. 3864-3868 ◽  
Author(s):  
Martina Wiesbauer ◽  
David Meierhofer ◽  
Johannes A. Mayr ◽  
Wolfgang Sperl ◽  
Bernhard Paulweber ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Primer Extension ◽  
Primer Extension Analysis ◽  
Mitochondrial Haplogroups ◽  
Extension Analysis

scholarly journals Transcript Analysis of Multiple Copies ofamo (Encoding Ammonia Monooxygenase) and hao(Encoding Hydroxylamine Oxidoreductase) in Nitrosomonas europaea

2001 ◽  
Vol 183 (3) ◽  
pp. 1096-1100 ◽  
Author(s):  
Norman G. Hommes ◽  
Luis A. Sayavedra-Soto ◽  
Daniel J. Arp
Keyword(s):  
Nitrosomonas Europaea ◽  
Primer Extension ◽  
Transcript Analysis ◽  
Ammonia Monooxygenase ◽  
Transcription Start ◽  
Hydroxylamine Oxidoreductase ◽  
Transcription Start Sites ◽  
Promoter Sequences ◽  
Genes Encoding ◽  
Multiple Copies

ABSTRACT The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and thec-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies ofamoC, the three copies of hao, and one copy ofhcy were cloned and sequenced. Primer extension reactions were done to identify transcription start sites for these genes, as well as for amoA. Putative ς70 promoter sequences were found associated with all but one of the mapped transcription start sites. Primer extensions were done withamoC primers using RNA harvested from cells incubated with and without ammonium. The experiments suggested that N. europaea cells may be able to use different promoters in the presence and absence of ammonium.

scholarly journals Organization of the 5′ region of the rat ATP citrate lyase gene

1994 ◽  
Vol 302 (3) ◽  
pp. 759-764 ◽  
Author(s):  
K S Kim ◽  
S W Park ◽  
Y A Moon ◽  
Y S Kim
Keyword(s):  
Genomic Library ◽  
Single Copy ◽  
Primer Extension ◽  
Primer Extension Analysis ◽  
Atp Citrate Lyase ◽  
Citrate Lyase ◽  
Sequence Elements ◽  
Flanking Region ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Extension Analysis

A genomic clone, encompassing the 5′ flanking region and the first seven exons of rat ATP citrate lyase gene, was isolated from a rat genomic library and sequenced. Primer-extension analysis showed that mRNA is transcribed at 4407 nucleotides upstream from the translation start site. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter used for transcription is identical in mammary gland, lung, liver, brain and kidney. Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. The 5′ flanking region contains several consensus sequences defined as promoter elements. These include a CAAT box and Sp1-binding sites. However, a TATA box lacks this promoter. The expression of the chloramphenicol acetyltransferase gene was induced by the 5‘ flanking region (-2370 to -1) in the CHO cell line. The 5′ flanking region also contains several sequence elements that may be involved in the transcriptional regulation of the gene.

scholarly journals Rapid detection of FGFR3 gene mutation in achondroplasia by DHPLC system-coupling heteroduplex and fluorescence-enhanced primer-extension analysis

2004 ◽  
Vol 49 (8) ◽  
pp. 399-403 ◽  
Author(s):  
Yi-Ning Su ◽  
Chien-Nan Lee ◽  
Shu-Chin Chien ◽  
Chia-Cheng Hung ◽  
Yin-Hsiu Chien ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Rapid Detection ◽  
Primer Extension ◽  
Primer Extension Analysis ◽  
Fgfr3 Gene ◽  
System Coupling ◽  
Extension Analysis

scholarly journals The ATP Synthase atpHAGDC(F1) Operon from Rhodobacter capsulatus

1998 ◽  
Vol 180 (2) ◽  
pp. 416-421 ◽  
Author(s):  
Roberto Borghese ◽  
Massimo Crimi ◽  
Luca Fava ◽  
Bruno Andrea Melandri
Keyword(s):  
Gene Transfer ◽  
Atp Synthase ◽  
Promoter Region ◽  
Rhodobacter Capsulatus ◽  
Growth Conditions ◽  
Primer Extension ◽  
Primer Extension Analysis ◽  
Viable Cells ◽  
Gene Transfer Agent ◽  
Extension Analysis

ABSTRACT The atpHAGDC operon of Rhodobacter capsulatus, containing the five genes coding for the F1 sector of the ATP synthase, has been cloned and sequenced. The promoter region has been defined by primer extension analysis. It was not possible to obtain viable cells carryingatp deletions in the R. capsulatus chromosome, indicating that genes coding for ATP synthase are essential, at least under the growth conditions tested. We were able to circumvent this problem by combining gene transfer agent transduction with conjugation. This method represents an easy way to construct strains carrying mutations in indispensable genes.

scholarly journals Multiplex Single-Nucleotide Primer Extension Analysis To Simultaneously Detect Eleven BRCA1 Mutations In Breast Cancer Families

2004 ◽  
Vol 50 (1) ◽  
pp. 203-206 ◽  
Author(s):  
Françoise Révillion ◽  
Alain Verdière ◽  
Joëlle Fournier ◽  
Louis Hornez ◽  
Jean-Philippe Peyrat
Keyword(s):  
Breast Cancer ◽  
Primer Extension ◽  
Primer Extension Analysis ◽  
Single Nucleotide ◽  
Brca1 Mutations ◽  
Single Nucleotide Primer Extension ◽  
Extension Analysis
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