In Vitro Model of Human Articular Cartilage Degradation

2003 ◽  
pp. 99-106 ◽  
Author(s):  
William D. Shingleton
Keyword(s):  
Articular Cartilage ◽  
In Vitro Model ◽  
Cartilage Degradation ◽  
Human Articular Cartilage ◽  
Vitro Model

scholarly journals Down-regulation of microRNA-203a suppresses IL-1β-induced inflammation and cartilage degradation in human chondrocytes through Smad3 signaling

2020 ◽  
Vol 40 (3) ◽  
Author(s):  
Yongbo An ◽  
Guang Wan ◽  
Jingang Tao ◽  
Mingxing Cui ◽  
Qinglan Zhou ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Inflammatory Response ◽  
In Vitro Model ◽  
Joint Inflammation ◽  
Cartilage Degradation ◽  
Inhibitory Effects ◽  
Key Factor ◽  
Ecm Degradation ◽  
Vitro Model

Abstract Osteoarthritis (OA) is a chronic and prevalent degenerative musculoskeletal disorder, which is characterized by articular cartilage degradation and joint inflammation. MicroRNA-203a (miR-203a) has been shown to be involved in multiple pathological processes during OA, but little is known about its function in chondrocyte extracellular matrix (ECM) degradation. In the present study, we aimed to elucidate the effects of miR-203a on articular cartilage degradation and joint inflammation. We observed that the miR-203a level was significantly up-regulated in OA tissues and in an in vitro model of OA, respectively. Inhibition of miR-203a significantly alleviated the interleukin (IL)-1β-induced inflammatory response and ECM degradation in chondrocytes. Moreover, mothers against decapentaplegic homolog 3 (Smad3), a key factor in maintaining chondrocyte homeostasis, was identified as a putative target of miR-203a in chondrocytes. More importantly, inhibition of Smad3 impaired the inhibitory effects of the miR-203a on IL-1β-induced inflammatory response and ECM degradation. Collectively, these results demonstrated that miR-203a may contribute to articular cartilage degradation of OA by targeting Smad3, suggesting a novel therapeutic target for the treatment of OA.

Novel Elements of the Chondrocyte Stress Response Identified Using an in Vitro Model of Mouse Cartilage Degradation

2016 ◽  
Vol 15 (3) ◽  
pp. 1033-1050 ◽  
Author(s):  
Richard Wilson ◽  
Suzanne B. Golub ◽  
Lynn Rowley ◽  
Constanza Angelucci ◽  
Yuliya V. Karpievitch ◽  
...  
Keyword(s):  
Stress Response ◽  
In Vitro Model ◽  
Cartilage Degradation ◽  
Vitro Model

scholarly journals An exploration of the ability of tepoxalin to ameliorate the degradation of articular cartilage in a canine in vitro model

2009 ◽  
Vol 5 (1) ◽  
pp. 25 ◽  
Author(s):  
Lisa Macrory ◽  
Anne Vaughan-Thomas ◽  
Peter D Clegg ◽  
John F Innes
Keyword(s):  
Articular Cartilage ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals An in vitro model for the pathological degradation of articular cartilage in osteoarthritis

2014 ◽  
Vol 47 (3) ◽  
pp. 645-652 ◽  
Author(s):  
Stephanie Grenier ◽  
Madhu M. Bhargava ◽  
Peter A. Torzilli
Keyword(s):  
Articular Cartilage ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals A Hyperosmolar Saline Solution Fortified with Anti-Inflammatory Components Mitigates Articular Cartilage Pro-Inflammatory and Degradative Responses in an In Vitro Model of Knee Arthroscopy

Cartilage ◽  
2021 ◽  
pp. 194760352110115
Author(s):  
Lasun O. Oladeji ◽  
Aaron M. Stoker ◽  
James P. Stannard ◽  
James L. Cook
Keyword(s):  
Articular Cartilage ◽  
Normal Saline ◽  
In Vitro Model ◽  
Saline Solution ◽  
Knee Arthroscopy ◽  
Femoral Condyle ◽  
Cartilage Explants ◽  
Anti Inflammatory ◽  
Vitro Model

Objective To evaluate differences in pro-inflammatory and degradative mediator production from osteoarthritic knee articular cartilage explants treated with a hyperosmolar saline solution supplemented with anti-inflammatory components (l-glutamine, ascorbic acid, sodium pyruvate, epigallocatechin gallate [EGCG], and dexamethasone) or normal saline using an in vitro model for knee arthroscopy. Design Full-thickness 6 mm articular cartilage explants ( n = 12/patient) were created from femoral condyle and tibial plateau samples collected from patients who received knee arthroplasty. One explant half was treated for 3 hours with hyperosmolar saline (600 mOsm/L) supplemented with anti-inflammatory components and the corresponding half with normal saline (308 mOsm/L). Explants were cultured for 3 days and then collected for biomarker analyses. Media biomarker concentrations were normalized to the wet weight of the tissue (mg) and were analyzed by a paired t test with significance set at P < 0.05. Results Cartilage was collected from 9 females and 2 males (mean age = 68 years). Concentrations of MCP-1 ( P < 0.001), IL-8 ( P = 0.03), GRO-α ( P = 0.02), MMP-1 ( P < 0.001), MMP-2 ( P < 0.001), and MMP-3 ( P < 0.001) were significantly lower in explant halves treated with the enhanced hyperosmolar solution. When considering only those cartilage explants in the top tercile of tissue metabolism, IL-6 ( P = 0.005), IL-8 ( P = 0.0001), MCP-1 ( P < 0.001), GRO-α ( P = 0.0003), MMP-1 ( P < 0.001), MMP-2 ( P < 0.001), MMP-3 ( P < 0.001), and GAG expression ( P = 0.0001) was significantly lower in cartilage explant halves treated with the enhanced hyperosmolar solution. Conclusions Treatment of cartilage explants with a hyperosmolar saline arthroscopic irrigation solution supplemented with anti-inflammatory components was associated with significant decreases in inflammatory and degradative mediator production and mitigation of proteoglycan loss.

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