scholarly journals A Hyperosmolar Saline Solution Fortified with Anti-Inflammatory Components Mitigates Articular Cartilage Pro-Inflammatory and Degradative Responses in an In Vitro Model of Knee Arthroscopy

Cartilage ◽  
2021 ◽  
pp. 194760352110115
Author(s):  
Lasun O. Oladeji ◽  
Aaron M. Stoker ◽  
James P. Stannard ◽  
James L. Cook
Keyword(s):  
Articular Cartilage ◽  
Normal Saline ◽  
In Vitro Model ◽  
Saline Solution ◽  
Knee Arthroscopy ◽  
Femoral Condyle ◽  
Cartilage Explants ◽  
Anti Inflammatory ◽  
Vitro Model

Objective To evaluate differences in pro-inflammatory and degradative mediator production from osteoarthritic knee articular cartilage explants treated with a hyperosmolar saline solution supplemented with anti-inflammatory components (l-glutamine, ascorbic acid, sodium pyruvate, epigallocatechin gallate [EGCG], and dexamethasone) or normal saline using an in vitro model for knee arthroscopy. Design Full-thickness 6 mm articular cartilage explants ( n = 12/patient) were created from femoral condyle and tibial plateau samples collected from patients who received knee arthroplasty. One explant half was treated for 3 hours with hyperosmolar saline (600 mOsm/L) supplemented with anti-inflammatory components and the corresponding half with normal saline (308 mOsm/L). Explants were cultured for 3 days and then collected for biomarker analyses. Media biomarker concentrations were normalized to the wet weight of the tissue (mg) and were analyzed by a paired t test with significance set at P < 0.05. Results Cartilage was collected from 9 females and 2 males (mean age = 68 years). Concentrations of MCP-1 ( P < 0.001), IL-8 ( P = 0.03), GRO-α ( P = 0.02), MMP-1 ( P < 0.001), MMP-2 ( P < 0.001), and MMP-3 ( P < 0.001) were significantly lower in explant halves treated with the enhanced hyperosmolar solution. When considering only those cartilage explants in the top tercile of tissue metabolism, IL-6 ( P = 0.005), IL-8 ( P = 0.0001), MCP-1 ( P < 0.001), GRO-α ( P = 0.0003), MMP-1 ( P < 0.001), MMP-2 ( P < 0.001), MMP-3 ( P < 0.001), and GAG expression ( P = 0.0001) was significantly lower in cartilage explant halves treated with the enhanced hyperosmolar solution. Conclusions Treatment of cartilage explants with a hyperosmolar saline arthroscopic irrigation solution supplemented with anti-inflammatory components was associated with significant decreases in inflammatory and degradative mediator production and mitigation of proteoglycan loss.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium

2010 ◽  
Vol 188 (3) ◽  
pp. 659-667 ◽  
Author(s):  
Thérèse Sergent ◽  
Neil Piront ◽  
Julie Meurice ◽  
Olivier Toussaint ◽  
Yves-Jacques Schneider
Keyword(s):  
Phenolic Compounds ◽  
Intestinal Epithelium ◽  
In Vitro Model ◽  
Anti Inflammatory ◽  
Vitro Model ◽  
Human Intestinal Epithelium

scholarly journals An exploration of the ability of tepoxalin to ameliorate the degradation of articular cartilage in a canine in vitro model

2009 ◽  
Vol 5 (1) ◽  
pp. 25 ◽  
Author(s):  
Lisa Macrory ◽  
Anne Vaughan-Thomas ◽  
Peter D Clegg ◽  
John F Innes
Keyword(s):  
Articular Cartilage ◽  
In Vitro Model ◽  
Vitro Model

Development of an in vitro model of injury-induced osteoarthritis in cartilage explants from adult horses through application of single-impact compressive overload

2013 ◽  
Vol 74 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Christina M. Lee ◽  
John D. Kisiday ◽  
C. Wayne McIlwraith ◽  
Alan J. Grodzinsky ◽  
David D. Frisbie
Keyword(s):  
In Vitro Model ◽  
Cartilage Explants ◽  
Single Impact ◽  
Vitro Model

scholarly journals Effects of copper and zinc on proteoglycan metabolism in articular cartilage

1996 ◽  
Vol 5 (2) ◽  
pp. 95-99 ◽  
Author(s):  
M. Pasqualicchio ◽  
R. Gasperini ◽  
G. P. Velo ◽  
M. E. Davies
Keyword(s):  
Articular Cartilage ◽  
Conditioned Medium ◽  
Degenerative Joint Disease ◽  
Cartilage Explants ◽  
Joint Disease ◽  
Inhibitory Effects ◽  
Pharmacological Agents ◽  
Copper And Zinc ◽  
Anti Inflammatory

Co-Cultures of porcine articular cartilage and synovium or synovial conditioned medium were used as anin vitromodel to mimic inflammatory events at the cartilage/synovial junction in degenerative joint disease. This model provides a useful tool to assess the anti-inflammatory and antiarthritic properties of pharmacological agents. In this study the effects of copper and zinc on (i) PG synthesis by cartilage and (ii) synovial-induced PG depletion have been investigated. Copper sulphate at a concentration of 0.01 mM did not stimulate PG synthesis significantly in cultured cartilage explants but completely abrogated the inhibitory effects of synovial tissue in co-culture experiments. This finding was supported by the histological demonstration of copper-dependent reversal of the PG depletion in cartilage exposed to synovial conditioned medium. Zinc sulphate at 0.01 mM had no effect on PG synthesis and was unable to protect cartilage against synovialinduced PG depletion. These results reveal possible mechanisms by which copper exerts its anti-inflammatory and anti-arthritic actions.

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