Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System

2004 ◽  
pp. 121-134
Author(s):  
Karim Nayernia ◽  
Wolfgang Engel
Keyword(s):  
In Vitro Spermatogenesis

scholarly journals Insights into in vitro spermatogenesis in mammals: Past, present, future

2017 ◽  
Vol 84 (7) ◽  
pp. 560-575 ◽  
Author(s):  
Amir Fattahi ◽  
Zeinab Latifi ◽  
Tohid Ghasemnejad ◽  
Hamid Reza Nejabati ◽  
Mohammad Nouri
Keyword(s):  
In Vitro Spermatogenesis

In vitro spermatogenesis of the mice in the collagen gel matrix

2003 ◽  
Vol 80 ◽  
pp. 47-48
Author(s):  
Jae-Ho Lee ◽  
Hae Kwan Kim ◽  
Sang Jin Lee ◽  
Kyoo-Wan Choi ◽  
Seoung Jae Lee
Keyword(s):  
Collagen Gel ◽  
In Vitro Spermatogenesis ◽  
Collagen Gel Matrix

scholarly journals In vitro spermatogenesis: A century-long research journey, still half way around

2018 ◽  
Vol 17 (4) ◽  
pp. 407-420 ◽  
Author(s):  
Mitsuru Komeya ◽  
Takuya Sato ◽  
Takehiko Ogawa
Keyword(s):  
In Vitro Spermatogenesis

scholarly journals A VOLATILE FACTOR IN RELATION TO IN VITRO SPERMATOGENESIS IN THE CECROPIA SILKWORM

1955 ◽  
Vol 109 (1) ◽  
pp. 64-74 ◽  
Author(s):  
MELVIN M. KETCHEL ◽  
CARROLL M. WILLIAMS
Keyword(s):  
In Vitro Spermatogenesis

scholarly journals Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takafumi Matsumura ◽  
Takuya Sato ◽  
Takeru Abe ◽  
Hiroyuki Sanjo ◽  
Kumiko Katagiri ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Culture Medium ◽  
Animal Species ◽  
Culture Method ◽  
Tension Control ◽  
Significant Progress ◽  
In Vitro Spermatogenesis ◽  
Lower Oxygen ◽  
Rat Spermatogenesis

AbstractIn vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we challenged IVS of rats with modifications of culture medium, by supplementing chemical substances, including hormones, antioxidants, and lysophospholipids. In addition, reducing oxygen tension by placing tissues in an incubator of lower oxygen concentration and/or applying silicone cover ceiling on top of the tissue were effective for improving the spermatogenic efficiency. Through these modifications of the culture condition, rat spermatogenesis up to round spermatids was maintained over 70 days in the cultured tissue. Present results demonstrated a significant progress in rat IVS, revealing conditions commonly favorable for mice and rats as well as finding rat-specific optimizations. This is an important step towards successful IVS in many animal species, including humans.

Sign in / Sign up

Export Citation Format

Share Document