In vitro spermatogenesis of the mice in the collagen gel matrix

2003 ◽  
Vol 80 ◽  
pp. 47-48
Author(s):  
Jae-Ho Lee ◽  
Hae Kwan Kim ◽  
Sang Jin Lee ◽  
Kyoo-Wan Choi ◽  
Seoung Jae Lee
Keyword(s):  
Collagen Gel ◽  
In Vitro Spermatogenesis ◽  
Collagen Gel Matrix

In vitro spermatogenesis by three-dimensional culture of rat testicular cells in collagen gel matrix

Biomaterials ◽  
2006 ◽  
Vol 27 (14) ◽  
pp. 2845-2853 ◽  
Author(s):  
Jae Ho Lee ◽  
Hyun Joo Kim ◽  
Haekwon Kim ◽  
Sang Jin Lee ◽  
Myung Chan Gye
Keyword(s):  
Three Dimensional ◽  
Collagen Gel ◽  
In Vitro Spermatogenesis ◽  
Testicular Cells ◽  
Three Dimensional Culture ◽  
Collagen Gel Matrix

Effects of IL-1β, TNF-α, and TGF-β on Proliferation of Human Nasal Epithelial Cells in Vitro

1998 ◽  
Vol 12 (4) ◽  
pp. 279-282 ◽  
Author(s):  
Yang-Gi Min ◽  
Chae-Seo Rhee ◽  
Sam-Hyun Kwon ◽  
Kang Soo Lee ◽  
Ja Bock Yun
Keyword(s):  
Epithelial Cells ◽  
Collagen Gel ◽  
Time Dependent ◽  
Dependent Manner ◽  
Primary Cells ◽  
Nasal Epithelial Cells ◽  
Human Nasal Epithelial Cells ◽  
Tnf Α ◽  
Collagen Gel Matrix

Previous reports suggest that cytokines may be involved in proliferation of the epithelium. The aim of this study was to determine the effects of cytokines, IL-1β, TNF-α, and TGF-β on proliferation of human nasal epithelial cells (HNECs) in vitro. Primary cells were cultured from HNECs on collagen gel matrix. Subcultured HNECs were incubated in a medium with recombinant human (rh) cytokines, rhIL-1β, rhTNF-a, and rhTGF-β at different concentrations of 0.01 ng/mL, 0.1 ng/mL, 1 ng/mL, 10 ng/mL, and 100 ng/mL. After 2-day incubation with these cytokines, daily cell proliferation was measured by MTT assay for 6 days. While rhIL-1β inhibited proliferation of HNECs in concentration-dependent and time-dependent manners, rhTNF-a stimulated HNEC growth at concentrations ranging from 0.01 ng/mL to 10 ng/mL in concentration-dependent and time-dependent manner. In contrast, rhTGF-b inhibited HNEC growth irrespective of concentration and incubation time. This study suggests that IL-1β, TNF-α, and TGF-β may have an important role in the repair of the nasal mucosa by regulating proliferation of the nasal epithelium.

Antibacterial Wound Healing Gels from Oligochitosan Salts

2012 ◽  
Vol 506 ◽  
pp. 31-34
Author(s):  
W. Janvikul ◽  
P. Ngamviriyavong ◽  
P. Uppanun ◽  
P. Tanjak ◽  
N. Sangjun
Keyword(s):  
Staphylococcus Epidermidis ◽  
Wound Closure ◽  
Collagen Gel ◽  
Antibacterial Activities ◽  
Dermal Fibroblasts ◽  
Collagen Gels ◽  
Collagen Gel Matrix ◽  
Growth Of Bacteria

Oligochitosan salt-based antibacterial wound gels were developed and evaluated in both in vitro and in vivo models. The antibacterial activities of the oligochitosan salts and the wound gels were investigated against Staphylococcus epidermidis RP625 and Escherichia coli ATCC 11775. The minimum inhibitory concentrations (MIC) of the oligochitosan salts were found in the range of 16-256 μg/mL. The wound gels demonstrated their in vitro activities on inhibiting the growth of bacteria. The 3-D collagen gel matrix containing human dermal fibroblasts cultured with each test gel was used as an in vitro model for the examination of cell proliferation and secretion of interleukin-8 (IL-8). The gels appeared to promote the proliferation and formation of cellular process of the fibroblasts in the 3-D collagen gels and stimulate the fibroblasts to produce more IL-8. In the in vivo model, it was noted that the gels could accelerate the wound closure process. The wounds were completely closed within 14 days.

In vitro Chemosensitivity Test for Human Genitourinary Tumors Using Collagen Gel Matrix

1993 ◽  
Vol 24 (2) ◽  
pp. 267-270 ◽  
Author(s):  
T. Uchibayashi ◽  
M. Egawa ◽  
H. Hisazumi ◽  
T. Hasegawa ◽  
T. Tanaka ◽  
...  
Keyword(s):  
Collagen Gel ◽  
Chemosensitivity Test ◽  
In Vitro Chemosensitivity ◽  
In Vitro Chemosensitivity Test ◽  
Collagen Gel Matrix

Collagen gel matrix assay as an in vitro chemosensitivity test for malignant astrocytic tumors

2007 ◽  
Vol 12 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Atsushi Ono ◽  
Hiroshi Kanno ◽  
Akimune Hayashi ◽  
Satoshi Nishimura ◽  
Yoshikazu Kyuma ◽  
...  
Keyword(s):  
Collagen Gel ◽  
Chemosensitivity Test ◽  
Astrocytic Tumors ◽  
In Vitro Chemosensitivity ◽  
In Vitro Chemosensitivity Test ◽  
Collagen Gel Matrix

scholarly journals Recombinant fish neurotrophin-6 is a heparin-binding glycoprotein: implications for a role in axonal guidance

1997 ◽  
Vol 324 (2) ◽  
pp. 461-466 ◽  
Author(s):  
Xiaoling LI ◽  
Juliane FRANZ ◽  
Friedrich LOTTSPEICH ◽  
Rudolf GÖTZ
Keyword(s):  
Neurite Outgrowth ◽  
Recombinant Baculovirus ◽  
Collagen Gel ◽  
Axonal Guidance ◽  
Heparin Binding ◽  
Protease Cleavage ◽  
Binding Glycoprotein ◽  
Collagen Gel Matrix

Neurotrophin-6 (NT-6) was identified in the teleost fish Xiphophorus as a new member of the neurotrophin gene family. NT-6 binds specifically the glycosaminoglycan heparin. In this study NT-6 was expressed in a stably transfected mammalian cell line, and in insect cells via a recombinant baculovirus. It was purified to homogeneity and characterized by MS and N-terminal sequencing. NT-6 from both expression systems was proteolytically processed at one of two protease cleavage motifs and was found to be glycosylated. It supported the survival of embryonic chick sensory neurons; half-maximal survival was observed at 100 ng/ml. Furthermore, NT-6 elicited neurite outgrowth in explanted embryonic dorsal root ganglia. Addition of heparin into the medium did not potentiate the activity of NT-6 in survival assays. However, when a sensory ganglion explant was cultured in a collagen gel matrix assay adjacent to a heparin bead coated with NT-6, neurite outgrowth directed towards the bead was observed. This indicated that NT-6 was slowly released from the heparin bead generating a concentration gradient of NT-6 instrumental for axonal guidance in vitro. Thus the interaction of NT-6 with heparin might not be required for the activation of the cellular receptor for NT-6 on responsive cells but rather may serve to control, in vivo, the distribution of NT-6.

scholarly journals In vitro Chemosensitivity Test of Human Gastric Carcinomas Using Collagen Gel Matrix

1991 ◽  
Vol 82 (5) ◽  
pp. 607-612 ◽  
Author(s):  
Shigekazu Ohyama ◽  
Motohiro Tanaka ◽  
Yutaka Yonemura ◽  
Kazuo Kinoshita ◽  
Itsuo Miyazaki ◽  
...  
Keyword(s):  
Collagen Gel ◽  
Chemosensitivity Test ◽  
Gastric Carcinomas ◽  
In Vitro Chemosensitivity ◽  
In Vitro Chemosensitivity Test ◽  
Collagen Gel Matrix

Insulin and IGF-I induce pronounced hypertrophy of skeletal myofibers in tissue culture

1991 ◽  
Vol 260 (3) ◽  
pp. C475-C484 ◽  
Author(s):  
H. H. Vandenburgh ◽  
P. Karlisch ◽  
J. Shansky ◽  
R. Feldstein
Keyword(s):  
Tissue Culture ◽  
Growth Factor ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Insulin Like Growth Factor ◽  
Cell Hyperplasia ◽  
Factor Ii ◽  
Igf I ◽  
Collagen Gel Matrix

Skeletal myofibers differentiated from primary avian myoblasts in tissue culture can be maintained in positive nitrogen balance in a defined serum-free medium for at least 6-7 days when embedded in a three-dimensional collagen gel matrix. Incubation of established myofiber cultures for 3-7 days with insulin (1 microM) or insulin-like growth factor I (IGF-I, 32 nM) stimulates both cell hyperplasia and myofiber hypertrophy. Mean myofiber diameter increases 71-98%. Insulin-like growth factor II stimulates cell hyperplasia but not myofiber hypertrophy. Cell growth results from a 42-62% increase in total protein synthesis and a 28-38% decrease in protein degradation. Myosin heavy-chain content increases 183-258% because of a 55% stimulation of myosin synthesis and 33-61% inhibition of degradation. Associated with myofiber hypertrophy is a 87-148% increase in the number of myofiber nuclei per unit myofiber length. The results indicate that insulin and IGF-I, but not IGF-II, can induce rapid myofiber hypertrophy in vitro, most likely by stimulating myoblast proliferation and/or fusion to established myofibers.

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