scholarly journals Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016

2019 ◽  
Vol 12 (3) ◽  
pp. 424-433 ◽  
Author(s):  
Musaed Abdulaziz Alsultan ◽  
Mohamed Ali Alhammadi ◽  
Maged Gomaa Hemida

Aim: This study aimed to isolate some of the currently circulating infectious bronchitis virus (IBV) strains from some broiler chicken farms in Al-Hasa and to do some molecular characteristics of these strains. Materials and Methods: We collected 300 tissue specimens, including the trachea, bronchi, lungs, and kidneys from some four commercial chicken farms showing respiratory manifestations. We tested these tissue specimens by the real-time polymerase chain reaction (RT-PCR) and gel-based PCR. We selected some PCR positive samples for isolation in the embryonated chicken eggs (ECE). We sequenced some PCR-positive samples and conducted phylogenetic analysis based on the obtained sequences. Results: Our molecular surveillance revealed that 31.6% of the tested specimens were IBV positive by PCR. We selected some positive specimens showing low Ct values by the qRT-PCR for virus isolation by the ECE. The infected eggs showed hemorrhage, dwarfing, and death in some cases after three passages in the ECE. We sequenced some of the positive PCR specimens and used the obtained sequences to draw the phylogenetic tree based on the partial IBV-ORF-1a, N, and S1 gene sequences. The phylogenetic trees based on the IBV-N and S1 gene sequences showed that the circulating IBV strains in Al-Hasa during 2016 was showing a high degree of identity to some strains from Taiwan and Italy. Meanwhile, the grouping of these strains based on the IBV-S1 sequences revealed that the currently circulating IBV strains in Al-Hasa belonged to Gr.I.7 along with strains from Taiwan. Conclusion: Our results confirmed the continuous circulation of the IBV among the chicken population in Al-Hasa despite the intensive application of vaccines against this virus.

1994 ◽  
Vol 34 (3) ◽  
pp. 327-338 ◽  
Author(s):  
Wang Li ◽  
Dave Junker ◽  
Lisa Hock ◽  
Elham Ebiary ◽  
Ellen W. Collisson

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Tamara Al-Jallad ◽  
Morshed Kassouha ◽  
Mohamad Salhab ◽  
Anouar Alomar ◽  
Mouhamad AL-Masalma ◽  
...  

Abstract Background Avian Infectious Bronchitis Virus (IBV) is a highly contagious disease that imposes a huge economic burden on the global poultry industry. IBV contains numerous serotypes and variants with incomplete tenuous cross immunological protection. The failure of currently used vaccines to protect against diverse, circulating IBV strains that are specific to a given region poses a major problem for the poultry industry. Thus, there is an urgent need to conduct studies aimed at genotyping field IB viruses. In this study, we have determined the molecular characteristics of circulating IBV by sequencing the S1 gene of viral isolates from affected previously vaccinated broiler flocks suffering from the disease. Results Ten isolates propagated in embryonated eggs showed an ability to induce typical IBV lesions after three successive viral passages. We performed a nested RT–PCR assay that targeted the hypervariable region 3 (HVR-3) of the S1 gene, and identified the isolates as IBV through sequence analysis. The IBV isolates showed sequence similarity between the Syrian isolates that vary from 96.20 to 100%, and those being closer to the Variant-2 strain IS/1494/06 (EU780077.2) with 97.5 to 99.4% similarities. However, less nucleotide identity was found with sequences belonging to the used vaccine strains such as H120, Mass5, and 4/91. Conclusions This study showed the presence of the Variant-2 strain circulating in Syrian broiler flocks showing signs of IBV disease. Currently, there is no commercial effective vaccine which protects birds against the Variant-2 strain. Continuous monitoring procedures should be taken to control and limit the spread of the IBV Variant-2 strain. This research emphasizes both the importance of epidemiological monitoring in intensive poultry farming for novel pathogens and the use of local isolates as future vaccine targets.


2014 ◽  
Vol 55 (3) ◽  
pp. 271-283 ◽  
Author(s):  
J. L. Chacón ◽  
M. S. Assayag ◽  
L. Revolledo ◽  
C. S. Astolfi-Ferreira ◽  
M. P. Vejarano ◽  
...  

2016 ◽  
Vol 60 (3) ◽  
pp. 596-602 ◽  
Author(s):  
Huseyin Yilmaz ◽  
Eda Altan ◽  
Utku Y. Cizmecigil ◽  
Aydin Gurel ◽  
Gulay Yuzbasioglu Ozturk ◽  
...  

Vaccine ◽  
2003 ◽  
Vol 21 (21-22) ◽  
pp. 2730-2736 ◽  
Author(s):  
Michael A. Johnson ◽  
Catherine Pooley ◽  
Jagoda Ignjatovic ◽  
Scott G. Tyack

2013 ◽  
Vol 98 (4) ◽  
pp. 1727-1735 ◽  
Author(s):  
Yan-Quan Wei ◽  
Hui-Chen Guo ◽  
Hu Dong ◽  
Hai-Ming Wang ◽  
Jin Xu ◽  
...  

Virus Genes ◽  
2014 ◽  
Vol 49 (2) ◽  
pp. 292-303 ◽  
Author(s):  
Keyu Feng ◽  
Yu Xue ◽  
Feng Wang ◽  
Feng Chen ◽  
Dingming Shu ◽  
...  

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