scholarly journals Novel RP-HPLC-PDA Approach for Efficient Simultaneous Quantification of Imipenem, Cilastatin and Relebactum in Bulk Drug and Injection Dose Forms

2021 ◽  
Vol 33 (4) ◽  
pp. 897-902
Author(s):  
Krishnaphanisri Ponnekanti ◽  
K. Sunitha
Keyword(s):  
Target Range ◽  
Array Detector ◽  
Photodiode Array Detection ◽  
Rp Hplc ◽  
Dipotassium Hydrogen Phosphate ◽  
Photodiode Array Detector ◽  
Injection Dose ◽  
Photodiode Array ◽  
Bulk Drug ◽  
Inactive Ingredients

In this investigation, a highly reliable, precise, stability indicating, specific and selective RP-HPLC approach with photodiode array detection (RP-HPLC-PDA) was established to determine simultaneously imipenem, cilastatin and relebactum in bulk drug and injection dose forms. Chromatographic separation of imipenem, cilastatin and relebactum was achieved via using C18 XTerra column and a mobile phase poised of acetonitrile and 0.1 M dipotassium hydrogen phosphate buffer (4.5 pH, set with 0.1% orthophosphoric acid) at 45:55 (v/v) ratio with a flow stream of 1 mL/min. The photodiode array detector was fixed at wavelength 245 nm and quantifications of imipenem, cilastatin and relebactum were based on assessing their peak response areas. Good linearity was detected in target range concentrations of 250-750 μg/mL (imipenem and cilastatin) and 125-375 μg/mL (relebactum). The precision (standard variation percentage) was between 0.141% and 0.257%. Accuracy (%assay nominal) determined was between 99.144% and 99.638%. The validated RP-HPLC approach was applied to Recarbio injection dose evaluating imipenem, cilastatin and relebactum content with no interference encountered from the injection dose inactive ingredients. Imipenem, cilastatin and relebactum were subjected to forced conditions like 30% peroxide, 0.1 N NaOH, sunlight, 0.1 N HCl and 60 ºC. Imipenem, cilastatin and relebactum were effectively separated, quantified and resolved from the degradants generated in forced conditions.

STABILITY INDICATING ASSAY METHOD OF SIROLIMUS USING HPLC AND SEPARATION OF ACID DEGRADANT BY HPTLC

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (04) ◽  
pp. 48-55
Author(s):  
S. Jadhav ◽  
◽  
P. Pisal ◽  
M. Mahajan
Keyword(s):  
Hplc Method ◽  
Assay Method ◽  
Array Detector ◽  
Oxidation Stress ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
The Given ◽  
Stressed Sample

A stability indicating RP-HPLC method has been developed and subsequently validated for Sirolimus. The proposed RP-HPLC method utilizes Phenomenex, C18, 3 μm, 4 mm x 150 mm column, mobile phase consisting of acetonitrile and water (65:35 V/V) and UV detection at 277 nm using a photodiode array detector in the stressed sample chromatograms. Crushed sirolimus tablets were exposed to thermal, photolytic, aqueous and oxidation stress conditions and stressed samples were analysed by the proposed method. Peak homogeneity data of the drug peaks were obtained using photodiode array detector. The stressed sample chromatograms demonstrated the specificity of the method for their estimation in presence of degradants. 99.66% degradation was observed in acid degradation study. on the other hand, no degradation was observed in aqueous condition. The given method was linear over a range of 0.1566 mg/mL to 0.4699 mg/mL. The mean recovery was found to be 99.23%. Acid degradant was separated by HPTLC and spectroscopic analysis was performed for the same.

scholarly journals HPLC Analysis of Kaempherol and Quercetin Derivatives Isolated by Different Extraction Techniques from Plant Matrix

2011 ◽  
Vol 94 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Krystyna Skalicka-Woźniak ◽  
Janusz Szypowski ◽  
Kazimierz Głowniak
Keyword(s):  
Hplc Analysis ◽  
Soxhlet Extraction ◽  
Array Detector ◽  
Extraction Techniques ◽  
Ultrasound Extraction ◽  
Quercetin Derivatives ◽  
Rp Hplc ◽  
Photodiode Array Detector ◽  
Photodiode Array

Abstract Soxhlet extraction, ultrasound extraction, and accelerated solvent extraction (ASE), followed by RP-HPLC with a photodiode array detector was used for the determination of flavonoids in fruits of Peucedanum alsaticum. Three compounds were identified: a kaempherol derivative (astragalin), and two quercetin derivatives (quercitrin and hiperoside). The highest extraction yields of the selected compounds were obtained by use of exhaustive ASE.

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR RILPIVIRINE HYDROCHLORIDE IN BULK AND USE IN ANALYSIS OF PREPARED NANOSUSPENSION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 42-46
Author(s):  
Madhuri Manchala ◽  
◽  
Vijaya Sri Kanagala ◽  
Ganapath Vinay Jain
Keyword(s):  
Homogenization Method ◽  
Hplc Method ◽  
Array Detector ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Development And Validation ◽  
Tablet Dosage

A simple, precise, accurate and robust RP-HPLC-PDA method was developed and validated for the determination of rilpivirine hydrochloride in tablet dosage forms. Reverse-phase chromatography was performed on a BDS hypersil (250 mm × 4.6 mm, 6 μm) column of Waters HPLC with Empower software and with a photodiode array detector. Methanol: acetonitrile: water 80:13.5:6.5 (v/v) was used as the mobile phase at a flow rate of 1 mL min-1 with PDA detection at 306 nm. Rilpivirine hydrochloride nanosuspension was prepared by using an ultrasonic homogenization method. Linearity was observed in the concentration range of 0.1–10 μg mL-1 with regression equation y = 508856X+46908 (R2 = 0.9998). The method was validated as per ICH guidelines. The RSD for intra-day (1.31- 0.67) and inter-day (1.69-1.59) precision was found to be less than 2%. The developed method is simple, precise and robust for the determination of rilpivirine hydrochloride and is successfully applied for the nanosuspension.

Toxicological screening of drugs by microbore high-performance liquid chromatography with photodiode-array detection and ultraviolet spectral library searches

1991 ◽  
Vol 37 (7) ◽  
pp. 1210-1215 ◽  
Author(s):  
A Turcant ◽  
A Premel-Cabic ◽  
A Cailleux ◽  
P Allain
Keyword(s):  
High Performance ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Array Detector ◽  
Photodiode Array Detection ◽  
Photodiode Array Detector ◽  
Degree Of Confidence ◽  
Photodiode Array ◽  
High Degree ◽  
Liquid Chromatographic

Abstract We use ultraviolet data, acquired with a photodiode-array detector coupled to a reversed-phase liquid-chromatographic system, to identify unknown drugs in plasma samples of acutely poisoned patients. Both retention time and spectra of the peaks obtained with a microbore Hypersil ODS column under gradient elution are compared with a library of approximately 350 compounds. We present our three-year experience with this system, which identifies drugs in less than 1 h, with a high degree of confidence.

Beta-Alanine and Tris-(hydroxyl methyl) Aminomethane as Peak Modifiers in the Development of RP–HPLC Methods Using Aceclofenac and Haloperidol Hydrochloride as Exemplar Drugs

2021 ◽  
Author(s):  
Ramalingam Peraman ◽  
Pokuri Chiranjeevi ◽  
Yerrigamreddy Padmanabha Reddy ◽  
Kondreddy Vinod Kumar ◽  
S G Vasantharaju ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Analytical Approach ◽  
Water Solubility ◽  
Array Detector ◽  
Beta Alanine ◽  
Rp Hplc ◽  
Null Effect ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Theoretical Plates

Abstract In the present analytical approach, beta-alanine (ALA) and tris-(hydroxyl methyl) aminomethane (TRIS) were investigated as peak modifiers due to their water solubility and their possible peak modifying a property. These reagents were tested for their efficacy on the elution of aceclofenac (ACF) and haloperidol hydrochloride (HLC) from C18 column (250 mm × 4.6 mm, 5 μ) equipped with a photodiode array detector. The test reagents were investigated at 0.25 ± 0.05% concentration with a varying % aqueous composition on elution efficacy of HLC and ACF. The added ALA/TRIS in the mobile phase significantly (P < 0.05) improvised the symmetrical elution of HLC with 3-fold theoretical plates increase (P < 0.05) and 10-fold reduced capacity factor as compared to the control run. For ACF, the shoulder effect observed for ACF peak was eliminated. The optimized mobile phase was a combination of acetonitrile and water containing 0.25% beta-alanine/TRIS (pH 3.5 with ortho-phosphoric acid) at the ratio of 70:30 and 60:40% v/v, respectively, for ACF and HLC. The method was validated as per ICHQ2 guidelines. The column performance was tested for reproducibility in non-peak modifier applications and revealed a null effect on the column, thus these agents are relatively less toxic to HPLC columns.

Simultaneous Determination of Co-administrated Deflazacort, Aprepitant and Granisetron in Dosage Forms and Spiked Human Plasma by RP-HPLC/PAD

2019 ◽  
Vol 57 (9) ◽  
pp. 790-798 ◽  
Author(s):  
Mahmoud A Tantawy ◽  
Soheir Alweshahy ◽  
Dalia A Elshabasy ◽  
Nadia F Youssef
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Array Detector ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Photodiode Array Detector ◽  
Photodiode Array

Abstract A selective reversed phase high performance liquid chromatography/photodiode array detector (RP-HPLC/PAD) method has been developed for simultaneous determination of the three co-administrated deflazacort, aprepitant and granisetron drugs used with chemotherapy. The three cited drugs have been chromatographed on C18 column using a mobile phase consisting of acetonitrile–0.2% v/v triethylamine (80:20 v/v, pH of 6.6 ± 0.05) with isocratic elution and monitored by photodiode array at 220 nm. International conference on harmonization (ICH) guidelines were followed to validate the developed method. Successful application of the developed method was assessed by the simultaneous determination of the studied drugs in pure forms, dosage forms and plasma samples in the ranges of 0.2–20, 0.4–40 and 0.2–20 μg/mL for deflazacort, aprepitant and granisetron, respectively.

scholarly journals RP-HPLC-DAD method for determination of olmesartan medoxomil in bulk and tablets exposed to forced conditions

2010 ◽  
Vol 60 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Ritesh Sharma ◽  
Shyam Pancholi
Keyword(s):  
Degradation Products ◽  
Olmesartan Medoxomil ◽  
Array Detector ◽  
Drug Degradation ◽  
Rp Hplc ◽  
Photolytic Degradation ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Tablet Dosage

RP-HPLC-DAD method for determination of olmesartan medoxomil in bulk and tablets exposed to forced conditionsA simple, sensitive and precise RP-HPLC-DAD method was developed and validated for the determination of olmesartan medoxomil (AT-II receptor blocker) in the presence of its degradation products. Olmesartan medoxomil and all the degradation products were resolved on a C18column with the mobile phase composed of methanol, acetonitrile and water (60:15:25,V/V/V, pH 3.5 by orthophosphoric acid) at 260 nm using a photodiode array detector. The method was linear over the concentration range of 1-18 μg mL-1and precise with RSD < 1 % in intra- and inter-day study. Excellent recoveries of 99.3 ± 0.9 to 100.8 ± 1.2% proved the accuracy of the method. Developed method was specific, as indicated by chromatographic resolution > 2.0 for each peak and sensitive withLOD0.03 μg mL-1andLOQ0.1 μg mL-1. The method was used to study the drug degradation behavior under forced conditions. Four degradation products (DP-I, II, III, IV) were formed during the degradation study in 0.1 mol L-1HCl whereas only DP-I, II and III were formed in water, 0.01 mol L-1NaOH and 3% H2O2. No significant thermal or photolytic degradation was observed in solid drug. The method was applied successfully for the assay of olmesartan medoxomil in the tablet dosage form.

Single procedure for detection, confirmation, and quantification of benzodiazepines in serum by liquid chromatography with photodiode-array detection

1991 ◽  
Vol 37 (5) ◽  
pp. 701-706 ◽  
Author(s):  
P R Puopolo ◽  
M E Pothier ◽  
S A Volpicelli ◽  
J G Flood
Keyword(s):  
Internal Standard ◽  
Reversed Phase ◽  
Array Detector ◽  
Photodiode Array Detection ◽  
Single Procedure ◽  
Flame Ionization ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Emergency Department Patients ◽  
Gas Chromatographic Method

Abstract We developed a reversed-phase chromatographic procedure for detecting benzodiazepines and other drugs in serum. A liquid-liquid extraction step with hexane/ethyl acetate isolates the drugs from serum; absolute recoveries are generally greater than 85%. Reconstituted extracts are chromatographed on a 4-microns (particle size) C18 column; 14 drugs and an internal standard (flunitrazepam) are separated in 8 min. Peak detection, purity checking, and identification are performed with a computerized photodiode-array detector. Run-to-run imprecision (CV) for many benzodiazepines is less than 3%. In a study of 126 specimens from Emergency Department patients, the procedure showed excellent agreement with a gas-chromatographic method involving either mass-spectrometric or flame-ionization detection. This single procedure provides rapid and accurate detection, quantification, and confirmation of benzodiazepines in serum.

scholarly journals A METHOD FOR DETERMINING 1,4-BENZOTHIAZINE DERIVATIVES IN RAT PLASMA BY HPLC AND ITS APPLICATION TO A PHARMACOKINETIC STUDY

2017 ◽  
Vol 9 (12) ◽  
pp. 82 ◽  
Author(s):  
Amit Rai ◽  
Vinit Raj ◽  
Ashok K. Singh ◽  
Amit K. Keshari ◽  
Sudipta Saha
Keyword(s):  
Pharmacokinetic Study ◽  
High Performance ◽  
Gradient Elution ◽  
Rat Plasma ◽  
Hplc Method ◽  
Array Detector ◽  
Hplc Separation ◽  
Rp Hplc ◽  
Photodiode Array Detector ◽  
Photodiode Array

Objective: The objective of the study was to develop, optimize and validate of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the determining 1,4-benzothiazine derivatives (AR13 and AR15) in a biological sample of rat plasma. The 1,4-benzothiazine derivatives are produced by the synthetic reactions.Methods: RP-HPLC separation was performed using an ODS-2 Hypersil column with gradient elution mobile phase consisting of water-acetonitrile for AR13 and AR15 (1:9 v/v, 3:7 v/v) at room temperature 1 ml/min flow rate, and interfaced with photodiode array detector (PDA) detector, 233 nm, 235 nm respectively.Results: A linear response was obtained between (range from 0.100-10.00 mg/ml) AR13 and (range from 0.096–9.88 mg/ml) AR15 with correlation coefficient 0.999 and 0.998. The linearity range of both AR13 and AR15 was 101.65±1.5 and 98.78±1.7.Conclusion: It was concluded that the method was simple, accurate, sensitive, accurate and reproducible and has been successfully applied to the pharmacokinetic study of AR13 and AR15 in rat plasma.

scholarly journals Development of a Validated Stability Indicating Method for Quantification of Amoxicillin, Clarithromycin and Lansoprazole in Bulk and Pharmaceutical Dosage form by RP-HPLC

2021 ◽  
pp. 207-220
Author(s):  
P. Sushma ◽  
A. K. M. Pawar ◽  
M. Divya
Keyword(s):  
High Performance ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Array Detector ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating Method ◽  
Rp Hplc ◽  
Photodiode Array Detector ◽  
System Suitability ◽  
Photodiode Array

Objective: The main objective of the present work is to develop an efficient, unique, reliable Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method for the simultaneous quantification of Amoxicillin (AMX), Clarithromycin (CTM) and Lansoprazole (LPZ) in bulk and pharmaceutical formulations.  Methods: The chromatographic separation was achieved by using Kinetex column C18 (100 x 4.6 mm, 2.6 µm) with Buffer (2.5 g of hexane sulphonic acid and 1ml of Triethylamine which are added to 1000 ml of HPLC water and adjusted its pH at 5.0 with Ortho phosphoric acid) and acetonitrile in the ratio of 70: 30 (%v/v) as a mobile phase at flow rate of 1.0 ml/min. The column effluents were monitored by a photodiode array detector at wavelength predetermined at 240 nm. Results: The method produced reliable results at optimized chromatographic conditions. The method was linear at concentration range of 15-225 µg/ml of AMX, 15-225 µg/ml of CTM and 0.9-13.5 µg/ml of LPZ with regression coefficients of 0.9999, 0.9999, and 0.9999 respectively. The retention times of AMX, CTM, LPZ were obtained as 1.513, 3.124, 3.770 min respectively. Results obtained for system suitability, precision, LOD and LOQ were in acceptable range and were validated according to the guidelines of the International Council for Harmonization (ICH). Conclusion: The proposed method was validated in accordance with ICH and all the obtained results were found satisfactory and were successfully applicable to the analysis of the bulk and the pharmaceutical formulations.

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