Comparison of Two Specimen-Collection and Microscopy Methods for the Rapid Laboratory Diagnosis of Seborrheic Dermatitis- A Pilot Study

The development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. The transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. It was also found to be an excellent enrichment medium for the selective isolation of Bordetella pertussis and B. parapertussis from scantily populated specimens. The investigation of 3,237 specimens that yielded 1,419 positive isolates of Bordetella, including 86 B. parapertussis, during a 20-month period is presented. A total of 3,076 specimens were processed in the laboratory by using the enrichment medium in addition to the routine procedure. Of these specimens, 757 were submitted in our medium, from which 137 (18%) were positive. Of the 567 specimens received in Amies transport medium, 290 (51%) positive cultures were obtained by the enrichment method only and not by primary culture.

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Pneumocystis cariniiPneumonia: An Approach to Rapid Laboratory Diagnosis

American Journal of Clinical Pathology ◽

10.1093/ajcp/72.1.90 ◽

1979 ◽

Vol 72 (1) ◽

pp. 90-93 ◽

Cited By ~ 8

Author(s):

Robert B. Cameron ◽

John C. Watts ◽

Bernard L. Kasten

Keyword(s):

Laboratory Diagnosis ◽

Pneumocystis Cariniipneumonia ◽

Rapid Laboratory

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Testing for Group A Streptococci

Archives of Pathology & Laboratory Medicine ◽

10.5858/2002-126-1467-tfgas ◽

2002 ◽

Vol 126 (12) ◽

pp. 1467-1470 ◽

Cited By ~ 1

Author(s):

Jane C. Dale ◽

Robert Novak ◽

Patricia Higgens ◽

Elmer Wahl

Keyword(s):

Laboratory Diagnosis ◽

Streptococcal Pharyngitis ◽

Test Results ◽

Self Assessment ◽

Small Hospital ◽

Physician Office ◽

Specimen Collection ◽

Group A ◽

Primary Plate ◽

Testing Practices

Abstract Context.—Laboratory diagnosis of group A streptococcal pharyngitis in physician office and small-hospital laboratories. Objective.—To characterize laboratory practices for the diagnosis of group A streptococcal pharyngitis and to identify opportunities for improvement. Design.—Voluntary self-assessment questionnaire, used to assess the laboratory practices of 790 laboratories subscribing to the College of American Pathologists Excel Microbiology Proficiency Testing Program. Results.—We observed discrepancies between self-reported and recommended specimen collection and laboratory testing practices for some laboratories. The most notable discrepancies were failing to provide a written specimen-collection procedure (17.8%), sampling the tongue and oral mucosa (2%), failing to always perform back-up cultures when rapid antigen test results were negative (57.5%), and finalizing culture reports within 24 hours or less (34.0%). Additionally, among those respondents who used the bacitracin disk, 57.9% (277 respondents) applied the disk directly onto a primary plate. Conclusions.—Opportunities exist to improve testing practices for the diagnosis of group A streptococcal pharyngitis for some physician office and small-hospital laboratories.

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A Pilot Study on Seborrheic Dermatitis Using Pramiconazole as a Potent Oral Anti-Malassezia Agent

Dermatology ◽

10.1159/000098577 ◽

2007 ◽

Vol 214 (2) ◽

pp. 162-169 ◽

Cited By ~ 20

Author(s):

Gérald E. Piérard ◽

Jannie Ausma ◽

Frédérique Henry ◽

Valérie Vroome ◽

Luc Wouters ◽

...

Keyword(s):

Pilot Study ◽

Seborrheic Dermatitis

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An Improved Method for Rapid Laboratory Diagnosis of Poliomyelitis

Experimental Biology and Medicine ◽

10.3181/00379727-106-26471 ◽

1961 ◽

Vol 106 (4) ◽

pp. 772-776

Author(s):

L. R. Peizer ◽

B. Mandei ◽

D. Weissman

Keyword(s):

Laboratory Diagnosis ◽

Improved Method ◽

Rapid Laboratory

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Mechanisms of Disseminated Intravascular Coagulation and its Rapid Laboratory Diagnosis

Laboratory Medicine ◽

10.1093/labmed/2.1.46 ◽

1971 ◽

Vol 2 (1) ◽

pp. 46-48

Author(s):

Newton J. Friedman

Keyword(s):

Disseminated Intravascular Coagulation ◽

Laboratory Diagnosis ◽

Intravascular Coagulation ◽

Rapid Laboratory

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Sample type is crucial to the diagnosis of Mycoplasma pneumoniae pneumonia by PCR

Journal of Medical Microbiology ◽

10.1099/jmm.0.45888-0 ◽

2005 ◽

Vol 54 (3) ◽

pp. 287-291 ◽

Cited By ~ 52

Author(s):

Riitta Räty ◽

Esa Rönkkö ◽

Marjaana Kleemola

Keyword(s):

Mycoplasma Pneumoniae ◽

Throat Swab ◽

Laboratory Diagnosis ◽

Nucleic Acid Amplification ◽

Rrna Gene ◽

Sample Type ◽

Diagnostic Efficacy ◽

Probe Hybridization ◽

Mycoplasma Pneumoniae Pneumonia ◽

Rapid Laboratory

Sensitive and specific methods for rapid laboratory diagnosis of Mycoplasma pneumoniae were not available until nucleic acid amplification methods were developed. The choice of sample type and method of sampling are crucial to optimal diagnostic efficacy. Three types of respiratory samples from 32 young military conscripts with pneumonia were collected during an outbreak of M. pneumoniae infection. Sputum, nasopharyngeal aspirate and throat swab specimens were tested by 16S rRNA gene-based PCR with liquid-phase probe hybridization, and the results were compared with serology. The PCR result was positive for 22 (69 %) of the sputa, 16 (50 %) of the aspirates and 12 (37.5 %) of the swabs. Serology with increasing or high titres supported the positive findings in all instances. Sputum, when available, is clearly the best sample type for young adults with pneumonia.

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Malassezia Species Associated Seborrheic Dermatitis and Its Comparison between HIV Positive and Negative Patients

International Journal of Health Sciences and Research ◽

10.52403/ijhsr.20211226 ◽

2021 ◽

Vol 11 (12) ◽

pp. 197-204

Author(s):

Harshita Hemant Sisodiya ◽

Nayana Avinash Ingole ◽

Shashir Wanjare

Keyword(s):

Skin Diseases ◽

Seborrheic Dermatitis ◽

Laboratory Diagnosis ◽

Hiv Positive ◽

Malassezia Species ◽

Pigmented Lesions ◽

Common Skin ◽

Male Preponderance ◽

Cellophane Tape ◽

Hiv Negative

Introduction: Malassezia yeasts are lipophilic organisms causing certain skin diseases. Seborrheic dermatitis (SD) is the second most common skin infection caused by Malassezia as well as in HIV/AIDS. Aim: To determine the frequency of association of Malassezia species in HIV infected and HIV non-infected patients with Seborrheic dermatitis. Materials and Methods: The prevalence of Seborrheic dermatitis is 5% in the general population. Hence a sample size of 80 was derived, 40 each of HIV seropositive and HIV seronegative adult patients clinically suspected of having Seborrheic Dermatitis. Specimens were collected by scraping and cellophane tape for KOH and Chicago Sky Blue (CSB) stain, and were cultured on Sabouraud’s dextrose agar. Data were analysed using SPSS version 16.0. P ≤ 0.05 was considered as significant. Results: Majority of the patients i.e. 46 (57.5%) out of 80 were in the age group of 18-30 years with male preponderance. All HIV positive patients with SD had scaly, greasy, itchy, hypo-pigmented and erythematous lesions, & neck (23) and groin (20) were commonest sites. In 39 HIV positive and 22 HIV negative patients, >2 sites were involved. Majority of the HIV negative patients with SD had scaly (40), itchy (24) and hypo-pigmented lesions (27) & dandruff, and scalp (24) & neck (18) were commonest sites. (P<0.05). Twenty HIV positive patients had CD4 count ranging from 200-350 cells/mm3. Malassezia was detected in 38 and 34 HIV positive & negative patients respectively in laboratory diagnosis. Conclusion: Seborrheic Dermatitis has severe presentation at multiple sites in HIV positive patients as compared to HIV negative patients. Key words: Malassezia, Seborrheic dermatitis, HIV positive, HIV negative.

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Disseminated Intravascular Coagulation During Severe Diseases in 0–12 Months old Children (Study on 54 Cases)

10.1055/s-0039-1689196 ◽

1975 ◽

Author(s):

E. Coeugniet

Keyword(s):

Disseminated Intravascular Coagulation ◽

Clinical Symptoms ◽

Laboratory Diagnosis ◽

Critical Periods ◽

Protamine Sulphate ◽

Intravascular Coagulation ◽

Gram Positive Bacteria ◽

K Deficiency ◽

Severe Infections ◽

Rapid Laboratory

Disseminated intravascular coagulation (DIC) occured during severe infections with: gram-negative bacteria (24 cases), gram-positive bacteria (3 cases), acute hemolysis (11 cases), pneumonias with hypoxic syndrome (16 cases). Adjuvant factors: Hypo-volaemia and metabolic acidosis (34 cases), malnutrition and hypoproteinaemia (32 cases). 38 patients were boys. Early clinical symptoms: alteration of the general state, impossibility of blood collectings because of hypercoagulability, bleeding after injections, haematemesis, melena, purpura, renal failure. Rapid laboratory diagnosis: ethanol test, paracoagulation with protamine sulphate, decrease of thrombocytes number, thrombin clotting time. The most important differential diagnosis is hypoprothrombinaenra by vit. K deficiency or by liver failure which could also complicate DIC (6 cases). During “critical” periods of diseases usually complicated by DIC the DIC prophylaxis is proposed (heparin 100–200 i.u./kg/day i.v. + dipyridamole 5 mg/kg/day i.v. or orally. The treatment of DIC: heparin 1000 i.u./kg/day i.v. or, in order to decrease the risk of secondary bleedings because of heparin an association: heparin 400 i.u./kg day i.v. + dipyridamole 5—10 mg/kg/day i.v. or orally.

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