Influence of au to- and xenogenic fibroblastes  and dermal equivalent on macrophage content in granulative tissue of ishemic cutaneus  wound on the 12 da y of regenerative histogenesis

The dermal equivalent (DE), a dermis substitute consisting of human skin fibroblasts growing into a three-dimensional collagen matrix, is extensively used in many applications: wound-healing response, pharmacological studies, skin grafting, fibroblast proliferation and migration, extracellular matrix remodeling, and efficacy of cosmetic products. The widespread growth of numerical modeling in biomechanical research has placed a heightened emphasis on accurate material property data for soft biological tissues, in particular for equivalent dermis which has not been so thoroughly investigated. Under unconfined compression loading, the effects of the strain rate, time culture, and cytoskeleton-disrupting agents are experimentally investigated. In order to model the observed mechanical behavior of the DE under the above conditions, the internal state variable approach is adopted for finite deformation viscoelasticity and the optimized material parameters are identified with respect to the stated thermodynamic restriction (i.e. positive viscous dissipation).

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Development of Mouse Fibroblast Dermal Equivalent Using Tissue Culture Technique

Animal Cell Technology: Basic & Applied Aspects ◽

10.1007/978-94-011-2844-5_75 ◽

1992 ◽

pp. 553-558

Author(s):

Tae-Boo Choe ◽

Jung-Keug Park ◽

Jae-Ho Lee ◽

Eun-Kyoung Yang

Keyword(s):

Tissue Culture ◽

Culture Technique ◽

Mouse Fibroblast ◽

Dermal Equivalent ◽

Tissue Culture Technique

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In vitro production of human dermal equivalent

Medicinski pregled ◽

10.2298/mpns1008459m ◽

2010 ◽

Vol 63 (7-8) ◽

pp. 459-464 ◽

Cited By ~ 1

Author(s):

Zoran Milosavljevic ◽

Biljana Ljujic

Keyword(s):

Vitamin C ◽

Collagen Type ◽

Collagen Type I ◽

Type I ◽

Collagen Gels ◽

In Vitro Production ◽

Dermal Equivalent ◽

Enzymatic Dissociation ◽

Vitro Production

Introduction. Human dermal tissue is composed of loose and dense connective tissue. Main cell populations are fibroblasts and the dominant fibers are built from collagen type I. The aim of our study was to determine the precise method and time frame for the in vitro production of human dermal equivalent and to investigate the effects of ratio of structural elements and vitamin C on characteristics of the engineered tissue. Material and methods. Primary isolation of the foreskin fibroblasts was performed by explant method and enzymatic dissociation. Various collagen gels were obtained by mixing cells (from 25x103 to 200x103/ml) and neutralized collagen type I (from 2 to 4 mg/ml), with or without vitamin C. The routine histological and morphometrical examination was performed. Results. Enzymatic dissociation of the foreskin proved to be a faster method for production of desired number of fibroblasts (7.5x105 for 4 days). The contraction of collagen-gels started from day one through day seven and was dependent on cell and collagen concentration with higher density gels being contracted to a greater extent, except for the lowest/highest values. The best result was achieved with 100x103 cells and 2 mg/ml collagen. Vitamin C at 50 ?g/ml had no effect on speed of tissue formation. Conclusion. A precise approach that mimic the in vivo conditions is needed for the in vitro production of the dermal equivalent suitable for the possible treatment of tissue defects. Nearly ten days are necessary from the donor tissue dissociation to the final product.

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