In this paper, the moisture susceptibility of different bituminous binders with two substrates (glass and limestone) was investigated. To that end, the tensile strength of different combinations of bituminous binder–substrate bond was measured using a pull-off test. This test was adapted from the pneumatic adhesion tensile testing instrument (PATTI) test to improve repeatability. Samples were tested in dry condition and after a 7-day conditioning in hot water bath (60 °C). An analysis of variance (ANOVA) was performed on the test results. Overall, the results show that in dry condition, the pull-off strength is a function of the bituminous binder type rather than of the substrate type. After water conditioning, an increase in the pull-off strength was observed for the bituminous binder without polymers and coupled with glass substrate. This was associated with an increase in binder stiffness. For the limestone substrate, the effect of water conditioning was significant only for one type of binder.
