In order to get clear cotton rustling sound signal, which will be analyzed to provide information at a certain stage of fabric processing for developing cotton products, before analysis of the data, it is the need for de-noising processing. The two de-noising methods, the median filtering method and the wavelet method are proposed in this paper. Their principles and algorithms are described. The cotton sound signals are exposed to interference and influence of some kinds of noise signal in the process of the data measured. So in practical applications, the two de-noising methods are used to process cotton rustling sound. The results of the process show the methods of processing the cotton sound signal are effective. The clear signal is getting to the next research.