scholarly journals Kloning Gen Transcriptional Activator Protein dan Replication-Associated Protein dari Squash leaf curl China virus Penyebab Penyakit Daun Kuning pada Tanaman Mentimun

2018 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Ni Nengah Putri Adnyani ◽  
I Dewa Made Putra Wiratama ◽  
I Gede Rai Maya Temaja ◽  
Sari Nurulita ◽  
Gede Suastika
Keyword(s):  
Transcriptional Activator ◽  
Plasmid Vector ◽  
Universal Primer ◽  
Activator Protein ◽  
Transcriptional Activator Protein ◽  
Cloning Method ◽  
Sequences Analysis ◽  
Dna Fragment ◽  
Important Disease ◽  
Leaf Curl

Yellow disease on Cucurbitaceae caused by Squash leaf curl China virus (SLCCNV; Begomovirus) is an important disease in many countries. Typical yellow leaf curl symptoms was found on cucumber in Tabanan, Bali. The objective of this research is to clone Transcriptional Activator Protein (TrAp) and Replication-Associated Protein (Rep) of SLCCNV Indonesian isolate. Universal primer for Begomovirus, i.e. SPG1/SPG2 was used to amplify DNA fragment of Begomovirus which cover the TrAp and Rep gene. The amplicon of ± 912 bp in size was then cloned into pTZ57R/T plasmid vector by TA-cloning method to obtain plasmid recombinant pTZ-SLCCNV. The plasmid recombinant was successfully confirmed by PCR colony and DNA sequencing. The nucleotide sequences analysis of plasmid recombinant showed highest homology (98%) of the corresponding gene to pumpkin isolate of SLCCNV from Malaysia.

scholarly journals The Hypersensitive Response to Tomato leaf curl New Delhi virus Nuclear Shuttle Protein Is Inhibited by Transcriptional Activator Protein

2007 ◽  
Vol 20 (12) ◽  
pp. 1581-1588 ◽  
Author(s):  
Mazhar Hussain ◽  
Shahid Mansoor ◽  
Shazia Iram ◽  
Yusuf Zafar ◽  
Rob W. Briddon
Keyword(s):  
Cell Death ◽  
Hypersensitive Response ◽  
Transcriptional Activator ◽  
Tomato Leaf ◽  
New Delhi ◽  
Nuclear Shuttle Protein ◽  
Activator Protein ◽  
Transcriptional Activator Protein ◽  
Tomato Leaf Curl ◽  
Leaf Curl

The hypersensitive response (HR) is a common feature of plant disease resistance reactions and a type of programmed cell death (PCD). Many pathogens are able to modulate pathways involved in cell death. In contrast to animal viruses, inhibitors of PCD activity have not been identified for plant-infecting viruses. Previously, we have reported that the nuclear shuttle protein (NSP) of Tomato leaf curl New Delhi virus (ToLCNDV) induces an HR in Nicotiana tabacum and Lycopersicon esculentum plants when expressed under the control of the Cauliflower mosaic virus 35S promoter. However, HR is not evident in plants infected with ToLCNDV, suggesting that the virus encodes a factor (or factors) that counters this response. Analysis of all ToLCNDV-encoded genes pinpointed the transcriptional activator protein (TrAP) as the factor mediating the anti-HR effect. Deletion mutagenesis showed the central region of TrAP, containing a zinc finger domain and nuclear localization signal, to be important in inhibiting the HR. These results demonstrate that TrAP counters HR-induced cell death, the first such activity identified for a plant-infecting virus.

scholarly journals Location, structure, and function of the target of a transcriptional activator protein.

1994 ◽  
Vol 8 (24) ◽  
pp. 3058-3067 ◽  
Author(s):  
H Tang ◽  
K Severinov ◽  
A Goldfarb ◽  
D Fenyo ◽  
B Chait ◽  
...  
Keyword(s):  
Transcriptional Activator ◽  
Structure And Function ◽  
Activator Protein ◽  
Transcriptional Activator Protein ◽  
And Function

scholarly journals Proline-independent binding of PUT3 transcriptional activator protein detected by footprinting in vivo.

1991 ◽  
Vol 11 (1) ◽  
pp. 564-567 ◽  
Author(s):  
J D Axelrod ◽  
J Majors ◽  
M C Brandriss
Keyword(s):  
Gene Product ◽  
Transcriptional Activator ◽  
Activator Protein ◽  
Transcriptional Activator Protein ◽  
Proline Utilization ◽  
Genes Encoding ◽  
Utilization Pathway

The PUT3 gene product is a transcriptional activator required for expression of the enzymes of the proline utilization pathway. Using two methods of footprinting in vivo, we have determined that PUT3 protein is poised at the promoters of the genes encoding these enzymes and that proline-mediated induction modulates the activity of constitutively bound PUT3.

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