scholarly journals Ammonium chloride administration prior to exercise has muscle‐specific effects on mitochondrial and myofibrillar protein synthesis in rats

2021 ◽  
Vol 9 (6) ◽  
Author(s):  
Amanda J. Genders ◽  
Evelyn C. Marin ◽  
Joseph J. Bass ◽  
Jujiao Kuang ◽  
Nicholas J. Saner ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Ammonium Chloride ◽  
Myofibrillar Protein ◽  
Specific Effects ◽  
Myofibrillar Protein Synthesis

scholarly journals Presleep dietary protein-derived amino acids are incorporated in myofibrillar protein during postexercise overnight recovery

2018 ◽  
Vol 314 (5) ◽  
pp. E457-E467 ◽  
Author(s):  
Jorn Trommelen ◽  
Imre W. K. Kouw ◽  
Andrew M. Holwerda ◽  
Tim Snijders ◽  
Shona L. Halson ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Dietary Protein ◽  
Myofibrillar Protein ◽  
Whole Body ◽  
Body Protein ◽  
Protein Ingestion ◽  
Casein Protein ◽  
The Impact ◽  
Myofibrillar Protein Synthesis

The purpose of this study was to determine the impact of ingesting 30 g casein protein with and without 2 g free leucine before sleep on myofibrillar protein synthesis rates during postexercise overnight recovery. Thirty-six healthy young men performed a single bout of resistance-type exercise in the evening (1945) after a full day of dietary standardization. Thirty minutes before sleep (2330), subjects ingested 30 g intrinsically l-[1-13C]phenylalanine-labeled protein with (PRO+leu, n = 12) or without (PRO, n = 12) 2 g free leucine, or a noncaloric placebo (PLA, n = 12). Continuous intravenous l-[ ring-2H5]phenylalanine, l-[1-13C]leucine, and l-[ ring-2H2]tyrosine infusions were applied. Blood and muscle tissue samples were collected to assess whole body protein net balance, myofibrillar protein synthesis rates, and overnight incorporation of dietary protein-derived amino acids into myofibrillar protein. Protein ingestion before sleep improved overnight whole body protein net balance ( P < 0.001). Myofibrillar protein synthesis rates did not differ significantly between treatments as assessed by l-[ ring-2H5]phenylalanine (0.057 ± 0.002, 0.055 ± 0.002, and 0.055 ± 0.004%/h for PLA, PRO, and PRO+leu, respectively; means ± SE; P = 0.850) or l-[1-13C]leucine (0.080 ± 0.004, 0.073 ± 0.004, and 0.083 ± 0.006%/h, respectively; P = 0.328). Myofibrillar l-[1-13C]phenylalanine enrichments increased following protein ingestion but did not differ between the PRO and PRO+leu treatments. In conclusion, protein ingestion before sleep improves whole body protein net balance and provides amino acids that are incorporated into myofibrillar protein during sleep. However, the ingestion of 30 g casein protein with or without additional free leucine before sleep does not increase muscle protein synthesis rates during postexercise overnight recovery.

scholarly journals Hypoenergetic diet-induced reductions in myofibrillar protein synthesis are restored with resistance training and balanced daily protein ingestion in older men

2015 ◽  
Vol 308 (9) ◽  
pp. E734-E743 ◽  
Author(s):  
Caoileann H. Murphy ◽  
Tyler A. Churchward-Venne ◽  
Cameron J. Mitchell ◽  
Nathan M. Kolar ◽  
Amira Kassis ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Resistance Training ◽  
Older Men ◽  
Myofibrillar Protein ◽  
Energy Restriction ◽  
Protein Distribution ◽  
Protein Meal ◽  
Fed State ◽  
Sarcoplasmic Protein ◽  
Myofibrillar Protein Synthesis

Strategies to enhance weight loss with a high fat-to-lean ratio in overweight/obese older adults are important since lean loss could exacerbate sarcopenia. We examined how dietary protein distribution affected muscle protein synthesis during energy balance (EB), energy restriction (ER), and energy restriction plus resistance training (ER + RT). A 4-wk ER diet was provided to overweight/obese older men (66 ± 4 yr, 31 ± 5 kg/m2) who were randomized to either a balanced (BAL: 25% daily protein/meal × 4) or skewed (SKEW: 7:17:72:4% daily protein/meal; n = 10/group) pattern. Myofibrillar and sarcoplasmic protein fractional synthetic rates (FSR) were measured during a 13-h primed continuous infusion of l-[ ring-13C6]phenylalanine with BAL and SKEW pattern of protein intake in EB, after 2 wk ER, and after 2 wk ER + RT. Fed-state myofibrillar FSR was lower in ER than EB in both groups ( P < 0.001), but was greater in BAL than SKEW ( P = 0.014). In ER + RT, fed-state myofibrillar FSR increased above ER in both groups and in BAL was not different from EB ( P = 0.903). In SKEW myofibrillar FSR remained lower than EB ( P = 0.002) and lower than BAL ( P = 0.006). Fed-state sarcoplasmic protein FSR was reduced similarly in ER and ER + RT compared with EB ( P < 0.01) in both groups. During ER in overweight/obese older men a BAL consumption of protein stimulated the synthesis of muscle contractile proteins more effectively than traditional, SKEW distribution. Combining RT with a BAL protein distribution “rescued” the lower rates of myofibrillar protein synthesis during moderate ER.

scholarly journals Rapid aminoacidemia enhances myofibrillar protein synthesis and anabolic intramuscular signaling responses after resistance exercise

2011 ◽  
Vol 94 (3) ◽  
pp. 795-803 ◽  
Author(s):  
D. W. West ◽  
N. A. Burd ◽  
V. G. Coffey ◽  
S. K. Baker ◽  
L. M. Burke ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Resistance Exercise ◽  
Myofibrillar Protein ◽  
Myofibrillar Protein Synthesis

Impact of Short-term Sedentariness on Week-to-Week Myofibrillar Protein Synthesis Rates in Physically Active Young Men

2018 ◽  
Vol 50 (5S) ◽  
pp. 370
Author(s):  
Brandon J. Shad ◽  
Andrew M. Holwerda ◽  
Yasir S. Elhassan ◽  
Luc J.C. van Loon ◽  
Janice L. Thompson ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Young Men ◽  
Myofibrillar Protein ◽  
Short Term ◽  
Physically Active ◽  
Myofibrillar Protein Synthesis

scholarly journals Improved recovery from skeletal muscle damage is largely unexplained by myofibrillar protein synthesis or inflammatory and regenerative gene expression pathways

2020 ◽  
Author(s):  
George Frederick Pavis ◽  
Tom SO Jameson ◽  
Marlou L. Dirks ◽  
Benjamin P. Lee ◽  
Doaa Reda Abdelrahman ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Muscle Damage ◽  
Muscle Function ◽  
Nutritional Intervention ◽  
Myofibrillar Protein ◽  
Skeletal Muscle Damage ◽  
Amino Acid Availability ◽  
Expression Pathways ◽  
Myofibrillar Protein Synthesis

The contribution of myofibrillar protein synthesis (MyoPS) to recovery from skeletal muscle damage in humans is unknown. Recreationally active males and females consumed a daily protein-polyphenol beverage targeted at increasing amino acid availability and reducing inflammation (PPB; n=9), both known to affect MyoPS, or an isocaloric placebo (PLA; n=9) during 168 h of recovery from 300 maximal unilateral eccentric contractions (EE). Muscle function was assessed daily. Muscle biopsies were collected 24, 27, 36, 72 and 168 h for MyoPS measurements using 2H2O and expression of 224 genes using RT-qPCR and pathway analysis. PPB improved recovery of muscle function, which was impaired for five days following EE in PLA (interaction; P<0.05). Acute postprandial MyoPS rates were unaffected by nutritional intervention (24-27 h). EE increased overnight (27-36 h) MyoPS versus control leg (PLA: 33±19%; PPB: 79±25%; leg P<0.01), and PPB tended to increase this further (interaction P=0.06). Daily MyoPS rates were greater with PPB between 72-168 h after EE, albeit after function had recovered. Inflammatory and regenerative signaling pathways were dramatically upregulated and clustered following EE but were unaffected by nutritional intervention. These results suggest that accelerated recovery from EE is not explained by elevated MyoPS or suppression of inflammation.

scholarly journals Blood Flow Restriction Combined With Low-load Resistance-type Exercise Increases Myofibrillar Protein Synthesis Rates

2018 ◽  
Vol 50 (5S) ◽  
pp. 645-646
Author(s):  
Jean Nyakayiru ◽  
Cas J. Fuchs ◽  
Joey S.J. Smeets ◽  
Annemie P. Gijsen ◽  
Joy P.B. Goessens ◽  
...  
Keyword(s):  
Blood Flow ◽  
Protein Synthesis ◽  
Load Resistance ◽  
Myofibrillar Protein ◽  
Blood Flow Restriction ◽  
Flow Restriction ◽  
Low Load ◽  
Myofibrillar Protein Synthesis

scholarly journals Blood Flow Restriction Only Increases Myofibrillar Protein Synthesis with Exercise

2019 ◽  
Vol 51 (6) ◽  
pp. 1137-1145 ◽  
Author(s):  
JEAN NYAKAYIRU ◽  
CAS J. FUCHS ◽  
JORN TROMMELEN ◽  
JOEY S. J. SMEETS ◽  
JOAN M. SENDEN ◽  
...  
Keyword(s):  
Blood Flow ◽  
Protein Synthesis ◽  
Myofibrillar Protein ◽  
Blood Flow Restriction ◽  
Flow Restriction ◽  
Myofibrillar Protein Synthesis

scholarly journals Failed Recovery of Glycemic Control and Myofibrillar Protein Synthesis With 2 wk of Physical Inactivity in Overweight, Prediabetic Older Adults

2017 ◽  
Vol 73 (8) ◽  
pp. 1070-1077 ◽  
Author(s):  
Chris McGlory ◽  
Mark T von Allmen ◽  
Tanner Stokes ◽  
Robert W Morton ◽  
Amy J Hector ◽  
...  
Keyword(s):  
Older Adults ◽  
Protein Synthesis ◽  
Glycemic Control ◽  
Physical Inactivity ◽  
Myofibrillar Protein ◽  
Myofibrillar Protein Synthesis
Sign in / Sign up

Export Citation Format

Share Document