scholarly journals High‐throughput determination of oxygen dissociation curves in a microplate reader—A novel, quantitative approach

2021 ◽  
Vol 9 (16) ◽  
Author(s):  
Simon Woyke ◽  
Mathias Ströhle ◽  
Hermann Brugger ◽  
Giacomo Strapazzon ◽  
Hannes Gatterer ◽  
...  
Keyword(s):  
High Throughput ◽  
Quantitative Approach ◽  
Oxygen Dissociation ◽  
Microplate Reader ◽  
Dissociation Curves

scholarly journals A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

2013 ◽  
Vol 8 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Everaldo Attard
Keyword(s):  
Phenolic Compounds ◽  
High Throughput ◽  
Extinction Coefficient ◽  
Rapid Determination ◽  
Excellent Correlation ◽  
Microtitre Plate ◽  
Spectrophotometric Methods ◽  
High Throughput Method ◽  
Microplate Reader

AbstractSeveral methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228±0.187x103 M−1 cm−1. The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.

Use of sodium borohydride in determination of oxygen dissociation curves of hemoglobin

1964 ◽  
Vol 7 (4) ◽  
pp. 393-400 ◽  
Author(s):  
Toshio Asakura ◽  
Yumiko Kawai ◽  
Yoshimasa Yoneyama ◽  
Haruhisa Yoshikawa
Keyword(s):  
Sodium Borohydride ◽  
Oxygen Dissociation ◽  
Dissociation Curves

scholarly journals Reliability of the determination of whole-blood oxygen affinity by means of blood-gas analyzers and multi-wavelength oximeters.

1989 ◽  
Vol 35 (5) ◽  
pp. 773-777 ◽  
Author(s):  
G Kwant ◽  
B Oeseburg ◽  
W G Zijistra
Keyword(s):  
Whole Blood ◽  
Carbon Monoxide Poisoning ◽  
Oxygen Affinity ◽  
Reference Interval ◽  
Accurate Method ◽  
Blood Gas ◽  
Oxygen Dissociation ◽  
Multi Wavelength ◽  
Dissociation Curves

Abstract Determination of the oxygen affinity of human whole blood with the help of blood-gas analyzers and multi-wavelength oximeters is compared with an accurate method for recording hemoglobin oxygen dissociation curves (Clin Chem 1982;28:1287-92). P50 (oxygen tension at half saturation; So2 = 50%) and Hill's n (delta log [So2/(1-So2)]/delta log Po2) were determined in blood of 24 healthy donors. Three slightly different procedures were used for determination of P50 on the basis of Po2, pH, Pco2, and So2 measured with each of four different blood-gas analyzer/oximeter combinations. These methods were not able to discriminate between high and low values for P50 within the normal reference interval, but never failed to detect the high oxygen affinity of blood stored for 12 days, reflected in low values of P50. The methods thus proved suitable for detecting clinically significant deviations in oxygen affinity such as occur in patients with, e.g., abnormal hemoglobins, anemias, or carbon monoxide poisoning. Determination of Hill's n by these methods did not produce useful results.

Oxygen dissociation curves for whole blood, recorded with an instrument that continuously measures pO2 and sO2 independently at constant t, pCO2, and pH.

1982 ◽  
Vol 28 (6) ◽  
pp. 1287-1292 ◽  
Author(s):  
A Zwart ◽  
G Kwant ◽  
B Oeseburg ◽  
W G Zijlstra
Keyword(s):  
Whole Blood ◽  
Data Acquisition System ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Measuring Chamber ◽  
Oxygen Dissociation ◽  
Constant Determination ◽  
Blood Specimens ◽  
Dissociation Curves

Abstract We describe a method for recording oxygen dissociation curves for whole-blood specimens. The blood sample is placed in a thermostated measuring chamber, and pO2 and SO2 are measured continuously by polarography and by reflectometry, respectively. During the recording of an oxygen dissociation curve, the pO2 and SO2 signals are stored in a data-acquisition system, while pH, pCO2, and temperature are kept constant. Determination of precision and error discussion indicated that the coefficient of variation (CV) of the determination of the oxygen dissociation curve is mainly determined by the error in the measurement of SO2. The overall CV of pO2 values belonging to the lower, mid-, and upper parts of the SO2 range is estimated to be about 2.6, 3.1, and 2.1%, respectively. In practice the measurements are about 30% more precise than estimated. With our method, the fixed-acid-induced Bohr effect (H+ factor) can be determined over the entire SO2 range with much greater precision than hitherto.

scholarly journals The Oxygen Dissociation and Alkaline Denaturation of Haemoglobins from two Species of earthworm

1958 ◽  
Vol 35 (2) ◽  
pp. 360-368
Author(s):  
T. M. HAUGHTON ◽  
G. A. KERKUT ◽  
K. A. MUNDAY
Keyword(s):  
Lumbricus Terrestris ◽  
Eisenia Foetida ◽  
Oxygen Dissociation ◽  
Partial Pressures ◽  
Alkaline Denaturation ◽  
Dissociation Curves ◽  
Earthworm Lumbricus

1. A method is described for determination of the oxygen dissociation curves of the blood of two species of earthworm, Lumbricus terrestris and Allolobophora terrestris. 2. The following values have been obtained for the partial pressures of oxygen (mm. Hg) required to saturate the blood: 3. Times for alkaline denaturation at pH 12.7 have been found as follows: Lumbricus terrestris 23 min., L. rubellus 2.5 min., L. festivus 11.5 min., Allolobophora terrestris 6 min., Eisenia foetida 5 min. 4. These results indicate definite differences in the properties of the haemoglobin of the species examined.

High-throughput determination of flavanone-O-glycosides in citrus beverages by paper spray tandem mass spectrometry

2021 ◽  
pp. 130060
Author(s):  
Fabio Mazzotti ◽  
Lucia Bartella ◽  
Ines Rosita Talarico ◽  
Anna Napoli ◽  
Leonardo Di Donna
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Tandem Mass ◽  
Paper Spray
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