Development of a micelle-enhanced high-throughput fluorometric method for determination of niclosamide using a microplate reader

Luminescence ◽  
2018 ◽  
Vol 34 (1) ◽  
pp. 48-54 ◽  
Author(s):  
Soad S. Abd El-Hay ◽  
Fathalla F. Belal
Keyword(s):  
High Throughput ◽  
Fluorometric Method ◽  
Microplate Reader

scholarly journals High‐throughput determination of oxygen dissociation curves in a microplate reader—A novel, quantitative approach

2021 ◽  
Vol 9 (16) ◽  
Author(s):  
Simon Woyke ◽  
Mathias Ströhle ◽  
Hermann Brugger ◽  
Giacomo Strapazzon ◽  
Hannes Gatterer ◽  
...  
Keyword(s):  
High Throughput ◽  
Quantitative Approach ◽  
Oxygen Dissociation ◽  
Microplate Reader ◽  
Dissociation Curves

Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening.

1989 ◽  
Vol 35 (10) ◽  
pp. 2112-2115 ◽  
Author(s):  
N S Gerasimova ◽  
I V Steklova ◽  
T Tuuminen
Keyword(s):  
Amino Acid ◽  
Human Plasma ◽  
High Throughput ◽  
Microplate Assay ◽  
Fluorometric Method ◽  
Amino Acid Analyzer ◽  
Screening Sensitivity ◽  
Blood Spot ◽  
Plasma Assay

Abstract We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay correlated well (r = 0.99) with results of amino acid analyzer determination of phenylalanine. When measurements are performed in an automated reader, the microplate assay has considerable advantages over conventional measurements in cuvettes: smaller volumes of reagents and automation enabling high throughput and convenience. Because the described method is a quantitative one, we can postulate that, compared with the semiquantitative Guthrie inhibition bioassay, this microplate assay is more reliable, easier to perform, and about twofold less costly.

scholarly journals A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

2013 ◽  
Vol 8 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Everaldo Attard
Keyword(s):  
Phenolic Compounds ◽  
High Throughput ◽  
Extinction Coefficient ◽  
Rapid Determination ◽  
Excellent Correlation ◽  
Microtitre Plate ◽  
Spectrophotometric Methods ◽  
High Throughput Method ◽  
Microplate Reader

AbstractSeveral methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228±0.187x103 M−1 cm−1. The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.

scholarly journals Dual-emission ratio fluorescence for selective and sensitive detection of ferric ions and ascorbic acid based on one-pot synthesis of glutathione protected gold nanoclusters

RSC Advances ◽  
2021 ◽  
Vol 11 (28) ◽  
pp. 17283-17290
Author(s):  
Shuai Zhang ◽  
Cong Zhang ◽  
Xiaodong Shao ◽  
Rentian Guan ◽  
Yingying Hu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gold Nanoclusters ◽  
Sensitive Detection ◽  
Dual Fluorescence ◽  
Ferric Ions ◽  
Fluorometric Method ◽  
One Pot ◽  
Dual Emission ◽  
One Pot Synthesis

A fluorometric method was proposed for the determination of Fe3+ and ascorbic acid (AA) based on blue and red dual fluorescence emissions of glutathione (GSH) stabilized-gold nanoclusters (AuNCs).

scholarly journals A FLUOROMETRIC METHOD FOR THE DETERMINATION OF THE 6-PYRIDONE OF N1-METHYLNICOTINAMIDE IN URINE

1949 ◽  
Vol 179 (1) ◽  
pp. 157-168 ◽  
Author(s):  
Fred. Rosen ◽  
William A. Perlzweig ◽  
Irwin G. Leder
Keyword(s):  
Fluorometric Method

High-throughput determination of flavanone-O-glycosides in citrus beverages by paper spray tandem mass spectrometry

2021 ◽  
pp. 130060
Author(s):  
Fabio Mazzotti ◽  
Lucia Bartella ◽  
Ines Rosita Talarico ◽  
Anna Napoli ◽  
Leonardo Di Donna
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Tandem Mass ◽  
Paper Spray
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