Determination of the Regulatory Disulfide Bonds of NADP-Dependent Malate Dehydrogenase from Pisum sativum by Site-Directed Mutagenesis

1997 ◽  
Vol 378 (9) ◽  
pp. 983-988 ◽  
Author(s):  
Robin Rießland ◽  
Rainer Jaenicke
Keyword(s):  
Pisum Sativum ◽  
Malate Dehydrogenase ◽  
Disulfide Bonds ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals Determination of the Catalytic Base in Family 48 Glycosyl Hydrolases

2011 ◽  
Vol 77 (17) ◽  
pp. 6274-6276 ◽  
Author(s):  
Maxim Kostylev ◽  
David B. Wilson
Keyword(s):  
Aspartic Acid ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Thermobifida Fusca ◽  
Glycosyl Hydrolases ◽  
Content Type ◽  
Modeling Data ◽  
Partial Activity

ABSTRACTThe catalytic base in family 48 glycosyl hydrolases has not been previously established experimentally. Based on structural and modeling data published to date, we used site-directed mutagenesis and azide rescue activity assays to show definitively that the catalytic base inThermobifida fuscaCel48A is aspartic acid 225. Of the tested mutants, only Cel48A with the D225E mutation retained partial activity on soluble and insoluble substrates. In azide rescue experiments, only the D225G mutation, in the smallest residue tested, showed an increase in activity with added azide.

Light-Regulation of Sorghum Leaf NADP-Malate Dehydrogenase. I — Site-Directed Mutagenesis

1992 ◽  
pp. 701-704
Author(s):  
Emmanuelle Issakidis ◽  
Jean-Pierre Jacquot ◽  
Myroslawa Miginiac-Maslow ◽  
Paulette Decottignies ◽  
Claude Crétin ◽  
...  
Keyword(s):  
Malate Dehydrogenase ◽  
Light Regulation ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals A thioredoxin-independent fully active NADP-malate dehydrogenase obtained by site-directed mutagenesis

FEBS Letters ◽  
1993 ◽  
Vol 321 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Emmanuelle Issakidis ◽  
Paulette Decottignies ◽  
Myroslawa Miginiac-Maslow
Keyword(s):  
Malate Dehydrogenase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis
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