scholarly journals Occurrence of tetracyclines in feedingstuffs – results of a two-year study within the official control of feed

2015 ◽  
Vol 59 (4) ◽  
pp. 527-532 ◽  
Author(s):  
Monika Przeniosło-Siwczyńska ◽  
Ewelina Patyra ◽  
Maja Chyłek-Purchała ◽  
Beata Kozak ◽  
Krzysztof Kwiatek
Keyword(s):  
Microbiological Method ◽  
Official Control ◽  
Feed Samples

Abstract The paper describes a microbiological method for the detection of antibacterial substances in feedingstuffs. The method allowed detection of the main antibiotic groups, including tetracyclines. In 2013-2014, a total of 171 feed samples were analysed to determine antibacterial substances. Among the analysed samples 84 (49.1%) were suspected to contain tetracyclines. Out of the 84 feeds analysed using chromatography, 28 (33.3%) contained undeclared tetracyclines, which were identified at concentrations ranging from 0.32 mg kg-1 to 48.98 mg kg-1.

Application of the CEN/ISO Standard for Phytase Activity Measurements to a New Phytase Product: Determination of a Robust Conversion Factor by an Interlaboratory Study for Screening Feed Samples

2019 ◽  
Vol 102 (6) ◽  
pp. 1808-1813
Author(s):  
María José González de la Huebra ◽  
Piotr Robouch ◽  
Håkan Emteborg ◽  
Stefano Bellorini ◽  
Aneta Cizek-Stroh ◽  
...  
Keyword(s):  
Conversion Factor ◽  
Feed Additives ◽  
Phytase Activity ◽  
Feed Additive ◽  
The European Union ◽  
Iso Standard ◽  
Official Control ◽  
Activity Measurements ◽  
Feed Samples

Abstract Background: Phytase-based preparations are important feed additives currently authorised in the European Union (EU). The European Standard (EN) and International Organization for Standardization (ISO) standard 30024 describes a harmonized method for the determination of phytase activity and is fit-for-purpose for official control of a group of phytase products. However, it is not suitable for the determination of the phytase activity of a new feed additive encoded as 4a16 in the EU Register of Feed Additives, to which a slightly different phytase activity definition has been attributed. Objective: To establish a robust conversion factor to support official control laboratories that apply the EN ISO method when monitoring feed products containing 4a16. Methods: The phytase activity of test materials was determined by the participants using the EN ISO and/or the “applicant” methods. Results: Robust relative SDs for repeatability and for reproducibility of the methods applied for the determination of the phytase activity in the materials containing the 4a16 feed additive ranged from 2.6 to 22% (EN ISO method) and from 2.4 to 39% (applicant method). Conclusions: The data obtained confirmed the performance characteristics published for other phytase-based feeds in the related standard methods. These results allowed us to estimate a factor of 2.68 to convert phytase activities measured with the EN ISO method into the enzyme activity measured with the applicant method. Highlights: The obtained conversion factor will allow EU official laboratories to screen feed samples supplemented with the 4a16 phytase by applying EN ISO Standard 30024.

Application of the CEN/ISO Standard for Phytase Activity Measurements to a New Phytase Product: Determination of a Robust Conversion Factor by an Interlaboratory Study for Screening Feed Samples

2019 ◽  
Vol 102 (6) ◽  
pp. 1808-1813
Author(s):  
María José González de la Huebra ◽  
Piotr Robouch ◽  
Håkan Emteborg ◽  
Stefano Bellorini ◽  
Aneta Cizek-Stroh ◽  
...  
Keyword(s):  
Conversion Factor ◽  
Feed Additives ◽  
Phytase Activity ◽  
Feed Additive ◽  
The European Union ◽  
Iso Standard ◽  
Official Control ◽  
Activity Measurements ◽  
Feed Samples

Background: Phytase-based preparations are important feed additives currently authorised in the European Union (EU). The European Standard (EN) and International Organization for Standardization (ISO) standard 30024 describes a harmonized method for the determination of phytase activity and is fit-for-purpose for official control of a group of phytase products. However, it is not suitable for the determination of the phytase activity of a new feed additive encoded as 4a16 in the EU Register of Feed Additives, to which a slightly different phytase activity definition has been attributed. Objective: To establish a robust conversion factor to support official control laboratories that apply the EN ISO method when monitoring feed products containing 4a16. Methods: The phytase activity of test materials was determined by the participants using the EN ISO and/or the “applicant” methods. Results: Robust relative SDs for repeatability and for reproducibility of the methods applied for the determination of the phytase activity in the materials containing the 4a16 feed additive ranged from 2.6 to 22% (EN ISO method) and from 2.4 to 39% (applicant method). Conclusions: The data obtained confirmed the performance characteristics published for other phytase-based feeds in the related standard methods. These results allowed us to estimate a factor of 2.68 to convert phytase activities measured with the EN ISO method into the enzyme activity measured with the applicant method. Highlights: The obtained conversion factor will allow EU official laboratories to screen feed samples supplemented with the 4a16 phytase by applying EN ISO Standard 30024.

scholarly journals Enumeration of Probiotic Bacilli Spores in Animal Feed: Interlaboratory Study

2003 ◽  
Vol 86 (3) ◽  
pp. 568-575 ◽  
Author(s):  
Renata G K Leuschner ◽  
Jan Bew ◽  
Armando Cruz ◽  
A Adler ◽  
E Auclair ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Animal Feed ◽  
Interlaboratory Study ◽  
Probiotic Bacteria ◽  
Precision Data ◽  
Official Control ◽  
Colony Forming Units ◽  
Relative Standard ◽  
Feed Samples

Abstract Fourteen out of 17 laboratories completed an interlaboratory study comparing 2 pretreatment protocols of feed samples containing authorized probiotic bacilli spores. Both methods used tryptone soy agar for enumeration. Pretreatment A involved preparation of a suspension of the feed sample in 50% ethanol. For pretreatment B, the sample was suspended in peptone salt solution and heated at 80°C for 10 min. Each laboratory analyzed 12 samples (6 per pretreatment), which represented duplicates of a high (109 colony-forming units [CFU]/g) and low (105 CFU/g) level of bacilli spores or a blank that contained vegetative probiotic bacteria only. For pretreatment A, the re-peatability relative standard deviation (RSDr) was 2.9% for the low level and 2.5% for the high. The reproducibility relative standard deviation (RSDR) values were 7.8 and 5.9%, respectively. Pretreatment B revealed RSDr values of 1.1 and 1.0%, and RSDR values of 5.8 and 3.4%, respectively. The heat treatment (pretreatment B) of feed samples had better precision data, resulted in higher viable bacilli counts, and was more effective in deactivating vegetative background flora. It is therefore recommended for adoption for official control purposes and for CEN and ISO standards.

Microbiological Method for Assaying Nystatin in Animal Feeds

1963 ◽  
Vol 46 (3) ◽  
pp. 438-444
Author(s):  
Joseph F Pagano
Keyword(s):  
Microbiological Assay ◽  
Microbiological Method ◽  
Animal Feeds ◽  
Assay Procedure ◽  
Feed Samples

Abstract A microbiological assay procedure for the determination of nystatin2 in animal feeds at levels as low as 2 5 ppm has been developed. The assay procedure and two feed samples were submitted to five collaborating laboratories for evaluation. The results of the collaborative assay study are presented, and it is recommended that the method be adopted as official, first action.

Enterococcal Virulence Determinants In Urinary Tract Infection Patients

2012 ◽  
Vol 6 (1) ◽  
pp. 14-17
Author(s):  
Jabin Akhter ◽  
Shaheda Anwar ◽  
Sharmeen Ahmed
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Virulence Determinants ◽  
Microtitre Plate ◽  
Microbiological Method ◽  
Enterococcal Infection ◽  
Microtitre Plate Assay ◽  
Positive Frequency

Urinary tract infection caused by Enterococci has become frequent occurrences in health care settings. Currently they emerged with increasing resistance to multiple antibiotics.  Haemolysin, gelatinase and biofilm production are some markers that have been proposed as possible Enterococcal virulence factors. In view of the increasing importance of Enterococcal infection, the present study was designed to isolate and identify the Enterococci to the species level from urine of urinary tract infection patients and to investigate their possible virulence factors. Biofilm was detected on polystyrene microtitre plate to see the adherence of microorganism. Haemolysin production and gelatin hydrolysis detected by standard microbiological method. Fifty nine enterococcal isolates were speciated by conventional microbiological method and examined for their ability to form biofilm by microtitre plate assay. In this study, biofilm formations by Enterococci were found in 83.33% isolates from catheterized and 56.09% from non-catheterized patients. Aong them, E.faecalis & 50% E.faecium produced biofilm. About 43.63% E.faecalis & 10% E.faecium produced haemolysin and only one isolate were found to be gelatinase positive. Frequency of virulence factors (VFs) in combination was observed in this study. Two VFs (haemolysin and biofilm) were observed in 27.11% in combination and 3 VFs ( haemolysinm biofilm and gelatinase) were present in 1.69% isolates. These results suggest that although there may not be an absolute role for individual virulence determinants in infectivity, combinations of factors may play a role in allowing a biofilm infection to be more resistant to therapy.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19361 Bangladesh J Med Microbiol 2012; 06(01): 14-17

Reports on Recent Activities of European Food Safety Authority

2019 ◽  
Vol 30 (5) ◽  
pp. 217-220
Keyword(s):  
Pesticide Residues ◽  
Food Products ◽  
Sampling Procedure ◽  
Control Programme ◽  
The European Union ◽  
Eu Member States ◽  
Official Control ◽  
National Programmes ◽  
Control Programmes ◽  
Risk Managers

This report provides an overview of the 2017 official control activities on pesticide residues carried out in the European Union (EU) Member States, Iceland and Norway. It summarises the results of both the 2017 EU-coordinated control programme (EUCP) and the national control programmes (NP). While the NPs are mostly risk based (so called enforcement samples) focusing on pesticides or products originating from countries where a number of exceedances have been observed in the past, the EUCP aims to present a statistically representative snapshot of the situation of pesticide residues in food products that are mostly consumed in the EU following a random sampling procedure. The report includes the outcome of a dietary risk assessment based on the results of the overall 2017 control programmes. The comprehensive analysis of the results of all reporting countries provides risk managers with sound-based evidence for designing future monitoring programmes, in particular for taking decisions on which pesticides and food products should be targeted in risk-based national programmes.

Microbiological Method for Eor

1990 ◽  
Vol 45 (1) ◽  
pp. 115-121 ◽  
Author(s):  
E. M. Yulbarisov
Keyword(s):  
Microbiological Method

Advances in standardizing and validating research techniques for pathogen monitoring in water

2004 ◽  
Vol 4 (2) ◽  
pp. 23-30
Author(s):  
K. Connell ◽  
M. Pope ◽  
K. Miller ◽  
J. Scheller ◽  
J. Pulz
Keyword(s):  
Validation Studies ◽  
Development Process ◽  
Monitoring Methods ◽  
Microbiological Method ◽  
Validation Process ◽  
Method Performance ◽  
Target Analytes ◽  
Interlaboratory Validation ◽  
Quality Control Criteria ◽  
Control Criteria

Designing and conducting standardized microbiological method interlaboratory validation studies is challenging because most methods are manual, rather than instrument-based, and results from the methods are typically subjective. Determinations of method recovery, in particular, are problematic, due to difficulties in assessing the true spike amount. The standardization and validation process used for the seven most recent USEPA 1600-series pathogen monitoring methods has begun to address these challenges. A staged development process was used to ensure that methods were adequately tested and standardized before resources were dedicated to interlaboratory validation. The interlaboratory validation studies for USEPA Method 1622, for Cryptosporidium, USEPA Method 1601 for coliphage, and USEPA Method 1605 for Aeromonas assessed method performance using different approaches, due the differences in the nature of the target analytes and the data quality needs of each study. However, the use of enumerated spikes in all of the studies allowed method recovery and precision to be assessed, and also provided the data needed to establish quantitative quality control criteria for the methods.

scholarly journals A MICROBIOLOGICAL METHOD FOR THE DETERMINATION OF NICOTINIC ACID

1941 ◽  
Vol 139 (2) ◽  
pp. 675-686 ◽  
Author(s):  
Esmond E. Snell ◽  
Lemuel D. Wright
Keyword(s):  
Nicotinic Acid ◽  
Microbiological Method
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