Assessment of AXL and mTOR genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shaghayegh Pishkari ◽  
Razie Hadavi ◽  
Ameneh Koochaki ◽  
Javad Razaviyan ◽  
Mahdi Paryan ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Target Genes ◽  
Critical Role ◽  
Luciferase Assay ◽  
Medullary Thyroid ◽  
Genes Expression ◽  
Before And After ◽  
New Strategy

Abstract Objectives The aim of the present study was to investigate the expression of AXL and mTOR genes and their targeting microRNAs (miRNAs) including miR-34a and miR-144 in Medullary Thyroid Carcinoma (MTC) cell line, TT, and determine the effect of these two miRNAs on their target genes to introduce new molecular markers or therapeutics. Methods The expression of miR-34a, miR-144, and their targets genes including AXL and mTOR was evaluated by quantitative Real-time PCR. Luciferase assay was performed to confirm the interaction between miRNAs and their target mRNAs. The expression level of AXL and mTOR was evaluated before and after miRNAs induction in TT cell line compared with Cos7 as control cells. Results The expression of AXL and mTOR were up-regulated significantly, while miR-34a and miR-144 were down-regulated in TT cell line compared to Cos7. After transduction, the overexpression of miR-34a and 144 caused down-regulation of both genes. Luciferase assay results showed that the mTOR is targeted by miR-34a and miR-144 and the intensity of luciferase decreased in the presence of miRNAs. Conclusions Based on the results of the present study and since AXL and mTOR genes play a critical role in variety of human cancers, suppression of these genes by their targeting miRNAs, especially miR-34a and miR-144, can be propose as a new strategy for MTC management. However, more studies are needed to approve the hypothesis.

scholarly journals Expression and processing of procholecystokinin in a rat medullary thyroid carcinoma cell line

1990 ◽  
Vol 271 (1) ◽  
pp. 31-36 ◽  
Author(s):  
L Ødum ◽  
J F Rehfeld
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Gel Chromatography ◽  
Medullary Thyroid ◽  
Thyroid Carcinoma Cell Line ◽  
Before And After ◽  
Thyroid Carcinoma Cell

A rat medullary thyroid carcinoma cell line, CA-77, was shown to express the cholecystokinin (CCK) gene. Measurements using a library of sequence-specific radioimmunoassays before and after enzymic treatment of extracts and chromatographic fractions showed that the cells contained 1.0 pmol of alpha-carboxyamidated cholecystokinins/10(6) cells, 0.4 pmol of glycine-extended intermediates/10(6) cells and 1.0 pmol of further C-terminal-extended pro-CCK/10(6) cells. Gel chromatography and reverse-phase h.p.l.c. revealed both sulphated and nonsulphated CCK-8 in the cells. The growth medium contained in addition alpha-amidated CCK-33, glycine-extended CCK-8 and pro-CCK. Exposure to 0.1 microM-dexamethasone for 6 days increased the cellular content and secretion of all of the described CCK peptides by 2-3-fold. The increase was first noted after 3 days of treatment. Monensin inhibited the synthesis of alpha-carboxyamidated CCK and the secretion of all of the CCK forms measured. Colchicine at a low concentration (0.2 mumol/l) apparently increased the synthesis and secretion of alpha-carboxyamidated CCK, whereas higher concentrations inhibited CCK synthesis. Finally, chloroquine inhibited the alpha-carboxyamidation of CCK. We conclude that the CA-77 cell line is a useful tool for studies of the expression and post-translational processing of pro-CCK.

Vitamin D levels and trabecular bone remodelling before and after surgery for medullary thyroid carcinoma

1984 ◽  
Vol 106 (3) ◽  
pp. 346-349 ◽  
Author(s):  
K. Emmertsen ◽  
F. Melsen ◽  
L. Mosekilde ◽  
Bi. Lund ◽  
Bj. Lund ◽  
...  
Keyword(s):  
Vitamin D ◽  
Thyroid Carcinoma ◽  
Trabecular Bone ◽  
Medullary Thyroid Carcinoma ◽  
Bone Remodelling ◽  
Vitamin D Metabolism ◽  
Medullary Thyroid ◽  
Number Of Patients ◽  
Vitamin D Levels ◽  
Before And After

Abstract. We previously reported increased mean serum 1,25-dihydroxyvitamin D (1,25-(OH)2D) and increased trabecular bone remodelling in patients with medullary thyroid carcinoma (MCT) and hypercalcitoninaemia. In the present paper we report that serum 1,25-(OH)2D and trabecular bone remodelling decreased following surgical cure for MCT and hypercalcitoninaemia in 4 patients despite no detectable post-surgical hypoparathyroidism or hypothyroidism. The results obtained in the present small number of patients suggest that the altered vitamin D metabolism and trabecular bone remodelling in patients with MCT is caused by the hypercalcitoninaemia.

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