The optimization and statistical analysis of fermentative hydrogen production using Taguchi method

Author(s):  
Sara Sepehri ◽  
Khosrow Rostami ◽  
Mehrdad Azin

AbstractThere are many variables influencing the performance of fermentative hydrogen production. The present paper tries to scrutinize and improve the rate and efficiency of fermentative hydrogen production through modeling and optimizing the effective parameters or variables on fermentative process. In this research, Taguchi experimental design method are applied to design the experiments. Then, the experimental results are analyzed by Design Expert software. Eventually, the optimization of process with considering the experimental data is implemented and their results are reported. The optimum conditions of bio hydrogen production and their predicted responses have been resulted in inoculums size of 11%, an initial glucose concentration of 10.13 g/L, initial pH of medium of 6.18 and FeSO4·7H2O concentration of 30 mg/L. The yield of hydrogen is 2.86 mol of hydrogen per mole. glucose with the production rate of 3.21 mol of hydrogen per hour.

2006 ◽  
Vol 52 (6) ◽  
pp. 525-532 ◽  
Author(s):  
Kaushik Nath ◽  
Anish Kumar ◽  
Debabrata Das

Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.31 mol (mol glucose)–1. However, at higher initial glucose concentration, both the rate and cumulative volume of hydrogen production decreased. The pH of 6.5 ± 0.2 at a temperature of 37 °C was found most suitable with respect to maximum rate of production of hydrogen in batch fermentation. Activation enthalpies of fermentation and that of thermal deactivation of the present process were estimated following a modified Arrhenius equation. The values were 47.34 and 118.67 kJ mol–1 K–1, respectively. The effect of the addition of Fe2+ on hydrogen production was also studied. It revealed that the presence of iron (Fe2+) in the media up to a concentration of 20 mg L–1 had a marginal enhancing effect on total hydrogen production. A simple model developed from the modified Gompertz equation was applied to estimate the hydrogen production potential, production rate, and lag-phase time in a batch process, based on the cumulative hydrogen production curves, using the software program Curve Expert 1.3. Key words: hydrogen, Enterobacter cloacae DM11, fermentation, glucose, H2 yield.


2014 ◽  
Vol 884-885 ◽  
pp. 433-436 ◽  
Author(s):  
Bo Wang ◽  
Ya Nan Yin ◽  
Rong Cheng ◽  
Qiong Zhang ◽  
Liang Wang ◽  
...  

The effect of SO2-4 concentration ranging from 0 to 10 g/L on fermentative hydrogen production by enriched anaerobic sludge was investigated using glucose as substrate at 35°C and initial pH 7.0. The experimental results showed that the hydrogen yield increased with increasing SO2-4 concentration from 0 to 0.05 g/L. The maximum maximum hydrogen yield of 272.2 mL/g glucose were obtained at the SO2-4 concentration of 0.05 g/L. The average hydrogen production rate increased with increasing SO2-4 concentration from 0 to 0.1 g/L and the maximum average hydrogen production rate of 8.4 mL/h was obtained at the SO2-4 concentration of 0.1 g/L. The Han-Levenspiel model could describe the effect of SO2-4 concentration on average hydrogen production rate successfully.


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