scholarly journals Non-Ferrous Metal Industry Waste Disposal Sites As A Source Of Poly-Extremotolerant Bacteria

2015 ◽  
Vol 14 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Peter Pristas ◽  
Zuzana Stramova ◽  
Simona Kvasnova ◽  
Jana Judova ◽  
Zuzana Perhacova ◽  
...  
Keyword(s):  
Waste Disposal ◽  
Abiotic Factors ◽  
Ferrous Metal ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Metal Industry ◽  
Disposal Site ◽  
Bacterial Strains ◽  
High Level ◽  
Ferrous Metal Industry

Abstract Waste disposal sites from non-ferrous metal industry constitute environments very hostile for life due to the presence of very specialized abiotic factors (pH, salt concentration, heavy metals content). In our experiments microflora of two waste disposal sites in Slovakia – brown mud disposal site from aluminium production near Ziar nad Hronom and nickel sludge disposal site near Sered - was analyzed for cultivable bacteria. Isolated bacteria were characterized by a combination of classical microbiological approaches and molecular methods and the most of isolated bacteria shown a poly-extremotolerant phenotype. The most frequently halotolerant (resistant to the high level of salt concentrations) and alkalitolerant (resistant to the high pH level) bacteria belonging to the Actinobacteria class were detected. The most of bacteria shown very high level of heavy metal resistance e.g. more than 500 μg/ml for Zn2+ or Cu2+. Based on our data, waste disposal sites thus on one side represents an important environmental burden but on other side they are a source of new poly-extremotolerant bacterial strains and species possibly used in many biotechnology and bioremediation applications.

The effects of technical change on carbon intensity in China’s non-ferrous metal industry

2021 ◽  
Vol 73 ◽  
pp. 102226
Author(s):  
Mei-Rui Zhong ◽  
Shun-Li Xiao ◽  
Han Zou ◽  
Yi-Jun Zhang ◽  
Yi Song
Keyword(s):  
Technical Change ◽  
Ferrous Metal ◽  
Metal Industry ◽  
Carbon Intensity ◽  
Ferrous Metal Industry

scholarly journals Fermentation pattern of the rumen and hindgut inocula of sheep grazing in an area polluted from the non-ferrous metal industry

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 66-72 ◽  
Author(s):  
Z. Váradyová ◽  
K. Mihaliková ◽  
S. Kišidayová ◽  
P. Javorský
Keyword(s):  
Rumen Fluid ◽  
Total Concentration ◽  
Ferrous Metal ◽  
In Vitro Study ◽  
Metal Industry ◽  
Rumen Content ◽  
Sheep Grazing ◽  
Metal Levels ◽  
Ferrous Metal Industry

In vitro study of the rumen fluid (RF) and hindgut content (HC) fermentation by microbiota taken from sheep grazing in an area atmospherically polluted from the non-ferrous metal industry was conducted and compared with controls from an uncontaminated area (UA). The experimental sheep were exposed to the prolonged intake of heavy metals by grazing in the contaminated area (CA) for one year. Soil and grass from that area and the rumen content of sheep were analyzed for heavy metal levels. Based on the levels of mercury (4.752 mg/kg), copper (232.9 mg/kg), cadmium (1.167 mg/kg), lead (92.509 mg/kg) and arsenic (74.59 mg/kg) the soil was categorized as profusely contaminated. Meadow hay (MH) from UA was used as a tested substrate of fermentation activity; it was incubated with buffered RF and HC inocula from CA and UA for 24 h. The gas volume in CA was significantly decreased by 50 and 36% in RF and HC, respectively. The methane production in CA was significantly decreased by 77 and 71% in RF and HC, respectively. The significantly decreased values of the fermentation parameters in CA in comparison with UA were accompanied by the reduced (P < 0.01) total concentration of rumen ciliate protozoa.  

scholarly journals Metagenomic deep sequencing reveals association of microbiome signature with functional biases in bovine mastitis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
M. Nazmul Hoque ◽  
Arif Istiaq ◽  
Rebecca A. Clement ◽  
Munawar Sultana ◽  
Keith A. Crandall ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Bovine Mastitis ◽  
Heavy Metal Resistance ◽  
Clinical Mastitis ◽  
Metal Resistance ◽  
Bacterial Strains ◽  
Viral Genomes ◽  
Milk Samples ◽  
Microbiome Diversity ◽  
Metagenome Sequencing

Abstract Milk microbiomes significantly influence the pathophysiology of bovine mastitis. To assess the association between microbiome diversity and bovine mastitis, we compared the microbiome of clinical mastitis (CM, n = 14) and healthy (H, n = 7) milk samples through deep whole metagenome sequencing (WMS). A total of 483.38 million reads generated from both metagenomes were analyzed through PathoScope (PS) and MG-RAST (MR), and mapped to 380 bacterial, 56 archaeal, and 39 viral genomes. We observed distinct shifts and differences in abundance between the microbiome of CM and H milk in phyla Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria with an inclusion of 68.04% previously unreported and/or opportunistic strains in CM milk. PS identified 363 and 146 bacterial strains in CM and H milk samples respectively, and MR detected 356 and 251 bacterial genera respectively. Of the identified taxa, 29.51% of strains and 63.80% of genera were shared between both metagenomes. Additionally, 14 archaeal and 14 viral genera were found to be solely associated with CM. Functional annotation of metagenomic sequences identified several metabolic pathways related to bacterial colonization, proliferation, chemotaxis and invasion, immune-diseases, oxidative stress, regulation and cell signaling, phage and prophases, antibiotic and heavy metal resistance that might be associated with CM. Our WMS study provides conclusive data on milk microbiome diversity associated with bovine CM and its role in udder health.

scholarly journals Zinc bioaccumulation by microbial consortium isolated from nickel smelter sludge disposal site

2017 ◽  
Vol 16 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Simona Kvasnová ◽  
Ľudmila Hamarová ◽  
Peter Pristaš
Keyword(s):  
Heavy Metal ◽  
Microbial Consortium ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Disposal Site ◽  
Good Growth ◽  
Lower Growth ◽  
Cultivable Bacteria ◽  
Sludge Disposal ◽  
Identification Of Bacteria

AbstractHeavy metal pollution is one of the most important environmental issues of today. Bioremediation by microorganisms is one of technologies extensively used for pollution treatment. In this study, we investigated the heavy metal resistance and zinc bioaccumulation by microbial consortium isolated from nickel sludge disposal site near Sereď (Slovakia). The composition of consortium was analyzed based on MALDI-TOF MS of cultivable bacteria and we have shown that the consortium was dominated by bacteria of genus Arthrobacter. While consortium showed very good growth in the zinc presence, it was able to remove only 15 % of zinc from liquid media. Selected members of consortia have shown lower growth rates in the zinc presence but selected isolates have shown much higher bioaccumulation abilities compared to whole consortium (up to 90 % of zinc removal for NH1 strain). Bioremediation is frequently accelerated through injection of native microbiota into a contaminated area. Based on data obtained in this study, we can conclude that careful selection of native microbiota could lead to the identification of bacteria with increased bioaccumulation abilities.

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