Application of real-time PCR for evaluation of distribution of equine herpesvirus type 1 in tissues of aborted fetuses

2015 ◽  
Vol 18 (4) ◽  
pp. 833-839 ◽  
Author(s):  
K. Stasiak ◽  
J. Rola ◽  
J.F. Zmudzinski
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Isoamyl Alcohol ◽  
Equine Herpesvirus ◽  
Viral Glycoprotein ◽  
Tissue Samples ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Abstract A highly sensitive and specific real-time PCR assay was used for detection and quantitation of equine herpesvirus type 1 (EHV-1) in the different internal organs of aborted fetuses. Tissue samples from 23 aborted fetuses submitted to the Department of Virology of the National Veterinary Research Institute in Pulawy between 2012 and 2013 were used for testing. Total DNA was extracted using a phenol-chloroform-isoamyl alcohol standard protocol. A real-time PCR with forward and reverse primers encompassing a highly conserved region encoding viral glycoprotein B was adapted for diagnosis of EHV-1 infection. The detection limit of the assay was shown to be 6.0×100 of viral DNA copies and the obtained standard curve exhibited a linear range from 100 to 107 molecules. Sixteen out of twenty three aborted fetuses (69.5%) were positive for EHV-1 in real-time PCR. The highest EHV-1 DNA load was obtained for liver (mean Ct value: 15.7) and lung (18.2) samples, while the lowest was in the thymus (29.6) and placenta (28.4).

Detection of equine herpesvirus type 1 by real time PCR

2006 ◽  
Vol 133 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Gabriella Elia ◽  
Nicola Decaro ◽  
Vito Martella ◽  
Marco Campolo ◽  
Costantina Desario ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Equine Herpesvirus ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Detection of equine herpesvirus type 1 using a real-time polymerase chain reaction

2006 ◽  
Vol 131 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Ibrahim S. Diallo ◽  
Glen Hewitson ◽  
Lucia Wright ◽  
Barry J. Rodwell ◽  
Bruce G. Corney
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Equine Herpesvirus ◽  
Chain Reaction ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1 ◽  
Polymerase Chain

Comparison of methods for the diagnosis of equine herpesvirus type 1 infection

2003 ◽  
Vol 51 (2) ◽  
pp. 153-163 ◽  
Author(s):  
László Szeredi ◽  
V. Pálfi ◽  
T. Molnár
Keyword(s):  
Inclusion Bodies ◽  
Virus Isolation ◽  
Immunohistochemical Method ◽  
Equine Herpesvirus ◽  
Intranuclear Inclusion ◽  
Tissue Samples ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

The objective of the investigations was to study the occurrence of the equine herpesvirus type 1 (EHV-1) infection in aborted equine fetuses and in newborn foals and to compare the sensitivity of virus isolation, immunohistochemistry and histology in 101 cases and of fetal serology in 68 cases in the diagnosis of the infection. Out of the 93 aborted equine fetuses and 8 weak foals, 15 (14.9%) (14 fetuses and 1 foal) proved to be EHV-1 infected by immunohistochemical and 13 (12.9%) by virological investigation. Characteristic microscopic changes were seen in several organs in all cases, while intranuclear inclusion bodies could be found only in 25 (35.2%) of the 71 virus-positive tissue samples. Four (5.9%) cases proved to be positive by fetal serological investigation, but none of these cases showed any EHV-1 specific lesions and in none of these cases could the virus be detected by virus isolation or by immunohistochemistry. According to the results, fetal serology does not seem to be a useful test in virus-positive cases, while the immunohistochemical method seems to be a reliable and a slightly more sensitive method than virus isolation in the diagnosis of EHV-1 infection.

The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection

2012 ◽  
Vol 15 (1) ◽  
pp. 151-153 ◽  
Author(s):  
A. Golke ◽  
J. Cymerys ◽  
A. Słońska ◽  
T. Dzieciątkowski ◽  
A. Chmielewska ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Response ◽  
Dynamic Monitoring ◽  
Equine Herpesvirus ◽  
Label Free ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Dermal Cell ◽  
Equine Herpesvirus Type 1

The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.

scholarly journals Evaluation of a vectored equine herpesvirus type 1 (EHV-1) vaccine expressing H3 haemagglutinin in the protection of dogs against canine influenza

Vaccine ◽  
2008 ◽  
Vol 26 (19) ◽  
pp. 2335-2343 ◽  
Author(s):  
Cristina Rosas ◽  
Gerlinde R. Van de Walle ◽  
Stephan M. Metzger ◽  
Karin Hoelzer ◽  
Edward J. Dubovi ◽  
...  
Keyword(s):  
Equine Herpesvirus ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1 ◽  
Canine Influenza

Late-Term Abortion, Stillbirth, and Neonatal Foal Death in Kyrgyzstan: First Isolation of Equine Herpesvirus Type 1 in the Country

2017 ◽  
Vol 51 ◽  
pp. 46-53
Author(s):  
Maksat Akhmedzhanov ◽  
Rysbek Nurgaziev ◽  
Jailobek Orozov ◽  
Irmgard Moser ◽  
Nikolaus Osterrieder ◽  
...  
Keyword(s):  
Equine Herpesvirus ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1
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