The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection

2012 ◽  
Vol 15 (1) ◽  
pp. 151-153 ◽  
Author(s):  
A. Golke ◽  
J. Cymerys ◽  
A. Słońska ◽  
T. Dzieciątkowski ◽  
A. Chmielewska ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Response ◽  
Dynamic Monitoring ◽  
Equine Herpesvirus ◽  
Label Free ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Dermal Cell ◽  
Equine Herpesvirus Type 1

The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.

Detection of equine herpesvirus type 1 by real time PCR

2006 ◽  
Vol 133 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Gabriella Elia ◽  
Nicola Decaro ◽  
Vito Martella ◽  
Marco Campolo ◽  
Costantina Desario ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Equine Herpesvirus ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Detection of equine herpesvirus type 1 using a real-time polymerase chain reaction

2006 ◽  
Vol 131 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Ibrahim S. Diallo ◽  
Glen Hewitson ◽  
Lucia Wright ◽  
Barry J. Rodwell ◽  
Bruce G. Corney
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Equine Herpesvirus ◽  
Chain Reaction ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1 ◽  
Polymerase Chain

Application of real-time PCR for evaluation of distribution of equine herpesvirus type 1 in tissues of aborted fetuses

2015 ◽  
Vol 18 (4) ◽  
pp. 833-839 ◽  
Author(s):  
K. Stasiak ◽  
J. Rola ◽  
J.F. Zmudzinski
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Isoamyl Alcohol ◽  
Equine Herpesvirus ◽  
Viral Glycoprotein ◽  
Tissue Samples ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Abstract A highly sensitive and specific real-time PCR assay was used for detection and quantitation of equine herpesvirus type 1 (EHV-1) in the different internal organs of aborted fetuses. Tissue samples from 23 aborted fetuses submitted to the Department of Virology of the National Veterinary Research Institute in Pulawy between 2012 and 2013 were used for testing. Total DNA was extracted using a phenol-chloroform-isoamyl alcohol standard protocol. A real-time PCR with forward and reverse primers encompassing a highly conserved region encoding viral glycoprotein B was adapted for diagnosis of EHV-1 infection. The detection limit of the assay was shown to be 6.0×100 of viral DNA copies and the obtained standard curve exhibited a linear range from 100 to 107 molecules. Sixteen out of twenty three aborted fetuses (69.5%) were positive for EHV-1 in real-time PCR. The highest EHV-1 DNA load was obtained for liver (mean Ct value: 15.7) and lung (18.2) samples, while the lowest was in the thymus (29.6) and placenta (28.4).

scholarly journals Evaluation of a vectored equine herpesvirus type 1 (EHV-1) vaccine expressing H3 haemagglutinin in the protection of dogs against canine influenza

Vaccine ◽  
2008 ◽  
Vol 26 (19) ◽  
pp. 2335-2343 ◽  
Author(s):  
Cristina Rosas ◽  
Gerlinde R. Van de Walle ◽  
Stephan M. Metzger ◽  
Karin Hoelzer ◽  
Edward J. Dubovi ◽  
...  
Keyword(s):  
Equine Herpesvirus ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1 ◽  
Canine Influenza

Late-Term Abortion, Stillbirth, and Neonatal Foal Death in Kyrgyzstan: First Isolation of Equine Herpesvirus Type 1 in the Country

2017 ◽  
Vol 51 ◽  
pp. 46-53
Author(s):  
Maksat Akhmedzhanov ◽  
Rysbek Nurgaziev ◽  
Jailobek Orozov ◽  
Irmgard Moser ◽  
Nikolaus Osterrieder ◽  
...  
Keyword(s):  
Equine Herpesvirus ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Recombinant Glycoprotein B of Equine herpesvirus Type 1 Elicits Protective Immune Response against Challenge in BALB/c Mouse Model

2021 ◽  
Author(s):  
Alok Joshi ◽  
R.P. Gupta ◽  
Selvaraj Pavulraj ◽  
Bidhan Chandra Bera ◽  
Taruna Anand ◽  
...  
Keyword(s):  
Immune Response ◽  
Plasmid Dna ◽  
Protective Immune Response ◽  
Glycoprotein B ◽  
Equine Herpesvirus ◽  
Recombinant Glycoprotein ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Background: Equine herpesvirus type 1 (EHV-1) is the most important viral pathogen of equines, causing respiratory illness, abortion, neonatal foal mortality and neurologic disorders. Large numbers of commercial EHV-1 vaccines are available to protect equines from the disease, but they provide only partial protection. Despite immunization with inactivated and modified live virus vaccine, mares show abortions. Present study was aimed to investigate the immunogenicity and protective efficacy of EHV-1 recombinant glycoprotein B (rgB) and gB expressing plasmid DNA against EHV-1 infection in BALB/c mice model.Methods: About 3-4 weeks old 225 female BALB/c mice were selected for the comparative study of immunization followed by challenged with EHV-1/India/Tohana/96-2 strain virus in 5 different groups of 45 animals each.Result: Following immunization, rgB vaccinated mice showed optimal stimulation of EHV-1 gB specific cell mediated and humoral mediated immunity (HMI and CMI). The gB expressing plasmid DNA vaccinated mice developed only CMI while inactivated whole virus vaccinated mice had only HMI. Upon EHV-1 challenge, all infected mice displayed variable levels of clinical signs with changes in body weight, however, vaccinated mice showed very rapid recovery with optimal protection. Positive control group mice showed severe pulmonary lesions along with persistence virus infection till 5 days post challenge (dpc) whereas vaccinated mice had less pulmonary lesion only up to 3dpc. Minimal lung lesions and early virus clearance was observed in the rgB immunized mice in comparison to the gB plasmid DNA and inactivated EHV-1 vaccine immunized mice. It has been concluded that immunization with rgB elicits optimum protective immune response against EHV-1 infection in mice model. The rgB could be a potential vaccine candidate against EHV-1 infection in equine in the future.

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