The Effect of Stereoisomeric Structure on Retention Time in Gas Liquid Chromatography of Per-O-trimethylsilyl Derivatives of Pentoses and Hexoses

1971 ◽  
Vol 26 (1) ◽  
pp. 24-25 ◽  
Author(s):  
Th. Gheorghiu ◽  
K. Oette
Keyword(s):  
Liquid Chromatography ◽  
Retention Time ◽  
Conformational Stability ◽  
Gas Liquid Chromatography ◽  
Oh Groups ◽  
Ring Form ◽  
Anomeric Carbon ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

The analysis of relationships between structure and retention time in gas liquid chromatography of pentoses and hexoses leads to the formulation of following rule: the per-O-trimethylsilyl derivatives of aldoses stereoisomers with conformational stability, identical ring form and the same number of substituted OH groups are longer retained on polar columns when the equatorial substituents are more numerous and are located nearer to the anomeric carbon.

scholarly journals Characterization and identification of 95 diphtheroid (group JK) cultures isolated from clinical specimens

1979 ◽  
Vol 9 (3) ◽  
pp. 418-424 ◽  
Author(s):  
P S Riley ◽  
D G Hollis ◽  
G B Utter ◽  
R E Weaver ◽  
C N Baker
Keyword(s):  
Liquid Chromatography ◽  
Antimicrobial Agents ◽  
Blood Cultures ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Clinical Specimens ◽  
Bacterial Cultures ◽  
Carbohydrate Fermentation ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Ninety-five cultures of group JK bacteria isolated from clinical specimens were characterized morphologically and biochemically. The microorganisms were isolated primarily from blood cultures. The bacterial cultures produced positive reactions when tested for catalase, Tween hydrolysis, and carbohydrate fermentation. Glucose and galactose were fermented by more than 90% of the organisms. Gas-liquid chromatography of trimethylsilyl derivatives of whole-cell hydrolysates of some of the group JK cultures yielded nearly identical elution profiles. The group JK microorganisms were susceptible to vancomycin but were resistant to most of the other 17 antimicrobial agents tested. A method is presented for differentiating the group JK microorganisms from other similar bacteria encountered in clinical specimens. Although these bacteria rarely occur in clinical specimens, they are capable of producing fatal infections (endocarditis and sepsis) in humans.

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