Anomalous Reduction of Cytochrome b in Highly Purified Complex III from Baker’s Yeast

1982 ◽  
Vol 37 (5-6) ◽  
pp. 445-447 ◽  
Author(s):  
Francisco F. de la Rosa ◽  
Graham Palmer
Keyword(s):  
Catalytic Amount ◽  
Baker’S Yeast ◽  
Complex Iii ◽  
Control Factor ◽  
Baker's Yeast ◽  
Cyt B ◽  
Pseudo First Order Reaction ◽  
Cyt C ◽  
Sulfur Protein ◽  
Two Phases

AbstractIn highly purified bc1-complex from baker’s yeast, the reduction of cyt c1 and partial reduction of cyt b is obtained by catalytic amount of succinate dehydrogenase and succinate in the presence of 7 μᴍ antimycin. After the addition of ferricyanide the c1 is re-oxidized and a increase in the reduction of b is observed. Using stopped-flow we established that the oxidation of c1 by ferricyanide proceeds as a pseudo-first order reaction and the reduction of b is faster and with two phases. Our observation suggests that these two processes are not directly interconected and that other component than c1 must be the “control factor” in the anomalous reduction of cyt b. This component must be, by exclusion, the iron-sulfur protein.

scholarly journals The preparation and characterization of highly purified, enzymically active complex III from baker's yeast.

1978 ◽  
Vol 253 (7) ◽  
pp. 2392-2399 ◽  
Author(s):  
J.N. Siedow ◽  
S. Power ◽  
F.F. de la Rosa ◽  
G. Palmer
Keyword(s):  
Baker’S Yeast ◽  
Complex Iii ◽  
Baker's Yeast ◽  
Active Complex

scholarly journals Coupling of Bioreaction and Separation via Novel Thermosensitive Ionic Liquids Applied in the Baker’s Yeast-Catalyzed Reduction of Ethyl 2-oxo-4-phenylbutyrate

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2056
Author(s):  
Yuexi Yang ◽  
Yugang Shi ◽  
Lifang Feng ◽  
Shiyi Tian
Keyword(s):  
Ionic Liquids ◽  
Baker’S Yeast ◽  
Mixed System ◽  
Phase Transformation Temperature ◽  
The Novel ◽  
Baker's Yeast ◽  
Transfer Resistance ◽  
System Temperature ◽  
Two Phases ◽  
Biphasic Systems

The use of baker’s yeast to reduce ethyl 2-oxo-4-phenylbutyrate (EOPB) in conventional biphasic systems is hindered by low productivities due to mass transfer resistance between the biocatalyst and the substrate partitioned into two different phases. To overcome the limitation, a new reaction-separation coupling process (RSCP) was configured in this study, based on the novel thermosensitive ionic liquids (ILs) with polyoxyethylene-tail. The solubility of ILs in common solvents was investigated to configure the unique thermosensitive ionic liquids–solvent biphasic system (TIBS) in which the reduction was performed. [(CH3)2N(C2H5)(CH2CH2O)2H][PF6] (c2) in 1,2-dimethoxyethane possesses the thermosensitive function of homogeneous at lower temperatures and phase separating at higher temperatures. The phase transformation temperature (PTT) of the mixed system of c2/1,2-dimethoxyethane (v/v, 5:18) was about 33 °C. The bioreaction takes place in a “homogeneous” liquid phase at 30 °C. At the end of each reduction run, the system temperature is increased upon to the PTT, while c2 is separated from 1,2-dimethoxyethane with turning the system into two phases. The enantiomeric excesses (e.e.) of ethyl (R)-2-hydroxy-4-phenylbutyrate ((R)-EHPB) increased about 25~30% and the yield of ethyl-2-hydroxy-4-phenylbutyrate (EHPB) increased 35% in TIBS, compared with the reduction in 1,2-dimethoxyethane. It is expected that the TIBS established in this study could provide many future opportunities in the biocatalysis.

scholarly journals Reductive titration of CoQ-depleted Complex III from Baker's yeast

FEBS Letters ◽  
1983 ◽  
Vol 163 (1) ◽  
pp. 140-143 ◽  
Author(s):  
F.F. de la Rosa ◽  
G. Palmer
Keyword(s):  
Baker’S Yeast ◽  
Complex Iii ◽  
Baker's Yeast

scholarly journals THREE IMPROVED METHODS FOR COCONUT OIL EXTRACTION

CORD ◽  
1993 ◽  
Vol 9 (01) ◽  
pp. 34
Author(s):  
Suhardiyono ◽  
Y.B. Che Man ◽  
B.A. Asbi ◽  
M.N. Azudin
Keyword(s):  
Acetic Acid ◽  
Coconut Oil ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Lower Phase ◽  
Saponification Value ◽  
Wet Processing ◽  
Combined Action ◽  
Two Phases ◽  
Improved Methods

Three methods for coconut oil extration using acetic acid, baker's yeast, and mixed enzymes were investigated. Coconut milk was allowed to settle for two hours; for cream separation. When the cream reacted with 25 % acetic acid at 0.l % ‑ 0.4 % levels or baker's yeast at 0.5 ‑ 2 g levels for 10 ‑ 14 hours, the oil was separated into two phases; the upper phase containing coconut oil‑rich fraction and the lower phase consisting of water. The oil phase was finally boiled for 20 minutes to remove moisture. The other extraction method was based on the combined action of cellulase, a ‑amylase, protease, and poly‑galacturonase at 0.1 % to 1 % on grated coconut meat at pH 4 to 8, 400C to 600C for 30 minutes. Oil recovely, moisture content, FFA, peroxide value, saponification value, anisidine value, iodine value and colour of the oil were studied. Up to 60 % recovery of high quality oil was obtained by acetic acid or baker's yeast treatment whilst that of mixed enzymes treatment was 73 %. These three alternatives wet processing showed significant improvement as compared to the traditional process.

COD REDUCTION OF BAKER'S YEAST WASTEWATER USING BATCH ELECTROCOAGULATION

2014 ◽  
Vol 13 (12) ◽  
pp. 3153-3160 ◽  
Author(s):  
Zakaria Al-Qodah ◽  
Mohammad Al-Shannag ◽  
Kholoud Alananbeh ◽  
Nahla Bouqellah ◽  
Eman Assirey ◽  
...  
Keyword(s):  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Cod Reduction ◽  
Yeast Wastewater
Sign in / Sign up

Export Citation Format

Share Document