A Spectroscopic Approach with Theoretical Studies to Study the Interaction of 9-aminoacridine with Certain Phenols

2017 ◽  
Vol 231 (5) ◽  
Author(s):  
Chandrakumar Manivannan ◽  
Rajadurai Vijay Solomon ◽  
Ponnambalam Venuvanalingam ◽  
Rajalingam Renganathan
Keyword(s):  
Steady State ◽  
Bathochromic Shift ◽  
Acetonitrile Solution ◽  
Theoretical Studies ◽  
Time Resolved Fluorescence ◽  
Quenching Rate ◽  
Time Resolved ◽  
Fluorescence Measurements ◽  
Quenching Rate Constant ◽  
Bimolecular Quenching

AbstractThe influence of phenols upon the fluorescence quenching of 9-aminoacridine (9-AA) was examined in acetonitrile solution by employing steady state and time-resolved fluorescence measurements. On increasing the concentration of quencher molecules the absorption spectra of 9-AA change with significant bathochromic shift. The fluorescence intensity of 9-AA change in presence of quencher molecules were measured at various temperatures as a function of the quencher concentrations. The observed bimolecular quenching rate constant (k

Concentration and Temperature Dependence of Laurdan Fluorescence in Glycerol

2003 ◽  
Vol 58 (9-10) ◽  
pp. 581-588 ◽  
Author(s):  
K. A. Kozyra ◽  
J. R. Heldt ◽  
J. Heldt ◽  
M. Engelkec ◽  
H. A. Diehl
Keyword(s):  
Steady State ◽  
Time Distribution ◽  
Emission Spectra ◽  
Fluorescence Decay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements ◽  
Fluorescence Decay Time ◽  
Laurdan Fluorescence ◽  
Vibrational Equilibrium

Steady-state and time-resolved fluorescence measurements have been performed on Laurdan, dissolved in viscous glycerol, as functions of temperature and concentration. The results indicate spectral heterogeneity of the Laurdan solution. The fluorescence decay time distribution is attributed to radiative deexcitation of spatial conformational forms of locally excited (LE) and charge transfer (CT) states, the S1(CT)EQ state being in thermodynamic and vibrational equilibrium. The lifetimes and contributions of the different fluorescence modes depend on concentration and temperature. The excitation and emission spectra show discontinuous changes with increase of the Laurdan concentration.We suppose that the observed changes are caused by the formation of Laurdan micelle aggregates.

scholarly journals ANG-2 for quantitative Na+determination in living cells by time-resolved fluorescence microscopy

2014 ◽  
Vol 13 (12) ◽  
pp. 1699-1710 ◽  
Author(s):  
Phillip Roder ◽  
Carsten Hille
Keyword(s):  
Steady State ◽  
Fluorescence Microscopy ◽  
Living Cells ◽  
Fluorescent Dye ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements

A new Na+-sensitive fluorescent dye ANG-2 was characterised photophysically (1P- and 2P-absorption, steady-state and time-resolved fluorescence) and its usability for quantitative Na+recordings in living cells by time-resolved fluorescence measurements was demonstrated.

Steady-state and time-resolved fluorescence measurements for studying molecular interactions: interaction of a calcium-binding probe with proteins

1996 ◽  
Vol 62 (1-3) ◽  
pp. 25-38 ◽  
Author(s):  
Kim Marie Hirshfield ◽  
Dmitri Toptygin ◽  
Gopal Grandhige ◽  
Hans Kim ◽  
Beverly Z. Packard ◽  
...  
Keyword(s):  
Steady State ◽  
Molecular Interactions ◽  
Calcium Binding ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements

scholarly journals Spectroscopic Study on the Reaction of Singlet-Excited Nile Blue with Certain Antioxidants

2021 ◽  
Author(s):  
C. Manivannan ◽  
S. Baskaran ◽  
Anbazhagan V
Keyword(s):  
Antioxidant Activity ◽  
Fluorescence Quenching ◽  
Electronic Properties ◽  
Nile Blue ◽  
Quenching Rate ◽  
Visible Absorption ◽  
Time Resolved ◽  
Fluorescence Measurements ◽  
Different Temperatures ◽  
Bimolecular Quenching

Abstract The photoinduced interaction of nile blue (NB) with various antioxidant molecules was investigated by fluorescence quenching technique and lifetime measurements. The various substituted catecholic compounds are employed as quenchers to evaluate their antioxidant activity. The formations of ground state complex between NB and quencher molecules observed from the UV-Visible absorption spectroscopy. The bimolecular quenching rate constants (kq) values depend on presence of substituent and its electronic properties of quencher molecules. Fluorescence quenching experiments have been performed at three different temperatures to assess the thermodynamic parameters. Time resolved fluorescence measurements suggest that the fluorescence quenching of NB with antioxidant molecules undergoes static quenching mechanism. The bond dissociation enthalpy (BDE) values reveal the discharge of HTfrom the antioxidant molecules. The electronic properties play an important role in the antioxidant activity of quencher molecules. The mechanism of fluorescence quenching between NB and quencher molecules are analysed based on the fluorescence quenching experiments, cyclic voltammetry experiments and BDE calculations.

Study between solvatochromism and steady-state and time-resolved fluorescence measurements of the Methylene blue in binary mixtures

2015 ◽  
Vol 119 ◽  
pp. 12-21 ◽  
Author(s):  
Luiza R.R. Santin ◽  
Sandra C. dos Santos ◽  
Diogo La Rosa Novo ◽  
Daniela Bianchini ◽  
Adriana P. Gerola ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Steady State ◽  
Binary Mixtures ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements
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