Study on products of enzymatic hydrolysis of pulp from poplar wood (Populus alba L.) Badanie produktów hydrolizy enzymatycznej masy celulozowej pozyskanej z drewna topoli (Populus alba L.)

Abstract Steam refining was investigated as a pretreatment for enzymatic hydrolysis of poplar wood from a short rotation plantation. The experiments were carried out without debarking to use an economically realistic raw material. Steam refining conditions were varied in the range of 3–30 min and 170–220°C, according to a factorial design created with the software JMP from SAS Institute Inc., Cary, NC, USA. Predicted steaming conditions for highest glucose and xylose yields after enzymatic hydrolysis were at 210°C and 15 min. Control tests under the optimized conditions verified the predicted results. Further pretreatments without bark showed that the enzymes were not significantly inhibited by the bark. The yield of glucose and xylose was 61.9% of theoretical for the experiments with the whole raw material, whereas the yield for the experiments without bark was 63.6%. Alkaline extraction of lignin from the fibers before enzymatic hydrolysis resulted in an increase of glucose yields from mild pretreated fibers and a decrease for severe pretreated fibers. The extracted lignin had a high content of xylose of up to 14% after very mild pretreatments. On the other hand, molecular weights of the extracted lignin increased substantially after pretreatments with a severity factor above 4. Hence, alkaline extraction of the lignin seems only attractive in a narrow range of steaming conditions.

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Synergistic effect of white-rot fungi and alkaline pretreatments for improving enzymatic hydrolysis of poplar wood

Industrial Crops and Products ◽

10.1016/j.indcrop.2016.03.041 ◽

2016 ◽

Vol 86 ◽

pp. 155-162 ◽

Cited By ~ 15

Author(s):

Liming Zhang ◽

Tingting You ◽

Tian Zhou ◽

Lu Zhang ◽

Feng Xu

Keyword(s):

Enzymatic Hydrolysis ◽

Synergistic Effect ◽

White Rot Fungi ◽

White Rot ◽

Poplar Wood ◽

Hydrolysis Of

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Effect of furfural on the enzyme activity during enzymatic hydrolysis of cellulose isolated from poplar wood (Populus sp.) Wpływ furfuralu na aktywność enzymu podczas hydrolizy celulozy wyodrębnionej z drewna topoli (Populus sp.)

PRZEMYSŁ CHEMICZNY ◽

10.15199/62.2015.11.7 ◽

2015 ◽

Vol 1 (11) ◽

pp. 67-70

Author(s):

Janusz Zawadzki

Keyword(s):

Enzyme Activity ◽

Enzymatic Hydrolysis ◽

Poplar Wood ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

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ENZYMATIC HYDROLYSIS OF FAST-GROWING POPLAR WOOD AFTER PRETREATMENT BY STEAM EXPLOSION

Cellulose Chemistry and Technology ◽

10.35812/cellulosechemtechnol.2021.55.52 ◽

2021 ◽

Vol 55 (5-6) ◽

pp. 637-647

Author(s):

ANNA GAŁĄZKA ◽

JAN SZADKOWSKI

Keyword(s):

Energy Source ◽

Chemical Composition ◽

Enzymatic Hydrolysis ◽

Natural Gas ◽

Steam Explosion ◽

Hard Coal ◽

Poplar Wood ◽

Wood Biomass ◽

Hydrolysis Of ◽

Heating Medium

The aim of the present study was to investigate the effect of steam explosion pretreatment, without maintaining the heating temperature, on the yield of enzymatic hydrolysis of wood biomass. Genetically modified poplar wood was used for the investigation. The pretreatment process was conducted at temperatures of 160 °C, 175 °C, 190 °C and 205 °C. Then, the system was rapidly decompressed. The heating medium was water. The chemical composition of biomass was determined before and after the steam explosion and then enzymatic hydrolysis was performed. The results of the chemical composition analysis showed a change in the holocellulose content in the analyzed biomass (about 80% for the native sample and 72% for the biomass sample treated at 205 °C), a decrease in the hemicelluloses content from about 40% (native sample) to 16% for the sample treated at 205 °C. The results of enzymatic hydrolysis showed the lowest glucose extraction efficiency for biomass hydrolysis after the treatment at 160 °C, of only about 9% compared to the theoretical content of glucose from the cellulose contained in hydrolysed wood biomass. The highest results were obtained for the samples treated at 190 °C and 205 °C. The study also estimated the processing costs, as a function of the heating medium (steam, water) and energy source (atomic energy, hard coal, natural gas, biomass), assuming heating with electric heaters. From the economic point of view, it is advantageous to use steam heating medium, and either natural gas or biomass as an energy source.

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Fungal treatment followed by FeCl3 treatment to enhance enzymatic hydrolysis of poplar wood for high sugar yields

Biotechnology Letters ◽

10.1007/s10529-013-1306-3 ◽

2013 ◽

Vol 35 (12) ◽

pp. 2061-2067 ◽

Cited By ~ 18

Author(s):

Wei Wang ◽

Tong Qi Yuan ◽

Bao Kai Cui

Keyword(s):

Enzymatic Hydrolysis ◽

Poplar Wood ◽

Fungal Treatment ◽

High Sugar ◽

Hydrolysis Of

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Steam pretreatment for enzymatic hydrolysis of poplar wood: comparison of optimal conditions with and without SO2 impregnation

Holzforschung ◽

10.1515/hf-2012-0076 ◽

2013 ◽

Vol 67 (1) ◽

pp. 9-17 ◽

Cited By ~ 14

Author(s):

Fokko Schütt ◽

Nils Peter Haas ◽

Laura Dehne ◽

Gerald Koch ◽

Ron Janzon ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Cell Walls ◽

Steam Pretreatment ◽

Poplar Wood ◽

Optimal Conditions ◽

Steam Refining ◽

Fiber Network ◽

Lignin Distribution ◽

Pretreatment Conditions ◽

Hydrolysis Of

Abstract Steam refining of non-debarked poplar wood with SO2 impregnation prior to steaming was investigated as pretreatment for enzymatic hydrolysis. Pretreatment conditions were varied in the range of 170°C–220°C, 3–30 min and 0.7–2.5% SO2 according to a factorial design. Predicted steaming conditions for highest carbohydrate yields after enzymatic hydrolysis were at 200°C, 15 min, and 2.5% SO2. The yield of glucose and xylose from control tests under these conditions was 43% representing an increase of 9% compared to results of former experiments without SO2 impregnation. Investigations on lignin extracted from the fibers revealed no distinct differences between pretreatment with and without SO2. No sulfonation occurred by the impregnation with SO2. Topochemical analyses of the fibers by cellular UV microspectrophotometry (UMSP) showed an inhomogeneous lignin distribution within the S2 of fibers after pretreatment without SO2 and local depositions of high UV-absorbing substances in the lumina of fibers and parenchyma cells. The lignin distribution of fiber cell walls after pretreatment with SO2 was more homogeneous with a preserved fiber network and only little amounts of deposited phenolic compounds in the lumina. Therefore, it might be concluded that the expulsion of lignin hinders the enzymes in accessing the cellulose.

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