scholarly journals GATA-3 Is Involved in the Development of Serotonergic Neurons in the Caudal Raphe Nuclei

1999 ◽  
Vol 19 (12) ◽  
pp. RC12-RC12 ◽  
Author(s):  
J. Hikke van Doorninck ◽  
Jacqueline van der Wees ◽  
Alar Karis ◽  
Erika Goedknegt ◽  
Michiel Coesmans ◽  
...  
Keyword(s):  
Raphe Nuclei ◽  
Serotonergic Neurons ◽  
Raphé Nuclei

Inescapable shock activates serotonergic neurons in all raphe nuclei of rat

2004 ◽  
Vol 153 (1) ◽  
pp. 233-239 ◽  
Author(s):  
Luiz F Takase ◽  
Maria Ines Nogueira ◽  
Michael Baratta ◽  
Sondra T Bland ◽  
Linda R Watkins ◽  
...  
Keyword(s):  
Inescapable Shock ◽  
Raphe Nuclei ◽  
Serotonergic Neurons ◽  
Raphé Nuclei

Physiological Identification of Pontomedullary Serotonergic Neurons in the Rat

1997 ◽  
Vol 77 (3) ◽  
pp. 1087-1098 ◽  
Author(s):  
Peggy Mason
Keyword(s):  
Discriminant Function ◽  
Discharge Rate ◽  
Raphe Nuclei ◽  
Discharge Pattern ◽  
Superior Olivary Complex ◽  
Noxious Stimulation ◽  
Serotonergic Neurons ◽  
Nucleus Reticularis ◽  
Serotonin Immunoreactivity ◽  
Raphé Nuclei

Mason, Peggy. Physiological identification of pontomedullary serotonergic neurons in the rat. J. Neurophysiol. 77: 1087–1098, 1997. Spinal serotonin is derived entirely from bulbar sources and plays an important role in spinal modulatory processes, including pain modulation. Establishing the electrophysiological properties of serotonergic bulbospinal neurons in the pontomedullary raphe and reticular formation is critical to understanding the physiological role of serotonin in the spinal cord. Neurons were characterized by their responses to noxious stimulation and their background discharge pattern in the lightly anesthetized rat. Characterized cells were intracellularly labeled with Neurobiotin, which was visualized with a Texas Red fluorophore. Sections containing the labeled cells were processed for serotonin immunocytochemistry with the use of a Bodipy fluorophore. Forty-seven intracellularly labeled cells were tested for serotonin immunoreactivity. The labeled neurons were located in raphe magnus, the nucleus reticularis magnocellularis, and the adjacent reticular and raphe nuclei at levels from the inferior olivary complex to the superior olivary complex. serotonergic cells were located in the raphe nuclei, in nucleus reticularis magnocellularis pars alpha, and in nucleus reticularis paragigantocellularis lateralis, but not in nucleus reticularis magnocellularis pars beta or nucleus reticularis gigantocellularis. Thirteen intracellularly labeled cells contained serotonin immunoreactivity. The background discharge rate of serotonergic cells averaged 1.8 Hz (range: 0.5–3.1 Hz). Discharge was steady and without sustained pauses or bursts in firing. Most serotonin-immunoreactive cells were unaffected or slightly excited by pinch and were unaffected by noxious heat. Three serotonergic cells were weakly excited by both noxious pinch and heat, whereas two serotonergic cells were briefly inhibited by these stimuli. Cells that lacked serotonin immunoreactivity were heterogeneous and included on, off, and neutral cells. Nonserotonergic cells differed from serotonergic cells in having an irregular discharge pattern and/or a high mean discharge rate. A linear discriminant function, employing background discharge characteristics as independent variables, was calculated that successfully classified 13 of 13 serotonergic and 32 of 33 nonserotonergic neurons. The probability of misclassification with the use of this discriminant function was estimated to be <10%. Employing the discriminant function on a test group of cells whose immunochemical content was unknown revealed a population of serotonergic-like cells that resembled the labeled serotonergic cells in background discharge pattern, response to noxious stimulation, and nuclear location. The discharge of pontomedullary serotonergic neurons is slow and steady, suggesting that these neurons may have a role in the tonic, rather than phasic, modulation of spinal processes.

scholarly journals Nonexocytotic serotonin release tonically suppresses serotonergic neuron activity

2015 ◽  
Vol 145 (3) ◽  
pp. 225-251 ◽  
Author(s):  
Boris Mlinar ◽  
Alberto Montalbano ◽  
Gilda Baccini ◽  
Francesca Tatini ◽  
Rolando Berlinguer Palmini ◽  
...  
Keyword(s):  
Serotonin Release ◽  
Raphe Nuclei ◽  
Neuron Activity ◽  
Release Mechanism ◽  
Serotonergic Neuron ◽  
Firing Activity ◽  
Serotonergic Neurons ◽  
Simple Diffusion ◽  
Inwardly Rectifying Potassium Channels ◽  
Raphé Nuclei

The firing activity of serotonergic neurons in raphe nuclei is regulated by negative feedback exerted by extracellular serotonin (5-HT)o acting through somatodendritic 5-HT1A autoreceptors. The steady-state [5-HT]o, sensed by 5-HT1A autoreceptors, is determined by the balance between the rates of 5-HT release and reuptake. Although it is well established that reuptake of 5-HTo is mediated by 5-HT transporters (SERT), the release mechanism has remained unclear. It is also unclear how selective 5-HT reuptake inhibitor (SSRI) antidepressants increase the [5-HT]o in raphe nuclei and suppress serotonergic neuron activity, thereby potentially diminishing their own therapeutic effect. Using an electrophysiological approach in a slice preparation, we show that, in the dorsal raphe nucleus (DRN), continuous nonexocytotic 5-HT release is responsible for suppression of phenylephrine-facilitated serotonergic neuron firing under basal conditions as well as for autoinhibition induced by SSRI application. By using 5-HT1A autoreceptor-activated G protein–gated inwardly rectifying potassium channels of patched serotonergic neurons as 5-HTo sensors, we show substantial nonexocytotic 5-HT release under conditions of abolished firing activity, Ca2+ influx, vesicular monoamine transporter 2–mediated vesicular accumulation of 5-HT, and SERT-mediated 5-HT transport. Our results reveal a cytosolic origin of 5-HTo in the DRN and suggest that 5-HTo may be supplied by simple diffusion across the plasma membrane, primarily from the dense network of neurites of serotonergic neurons surrounding the cell bodies. These findings indicate that the serotonergic system does not function as a sum of independently acting neurons but as a highly interdependent neuronal network, characterized by a shared neurotransmitter pool and the regulation of firing activity by an interneuronal, yet activity-independent, nonexocytotic mechanism.

scholarly journals A Whole-Brain Atlas of Inputs to Serotonergic Neurons of the Dorsal and Median Raphe Nuclei

Neuron ◽  
2014 ◽  
Vol 83 (3) ◽  
pp. 663-678 ◽  
Author(s):  
Iskra Pollak Dorocic ◽  
Daniel Fürth ◽  
Yang Xuan ◽  
Yvonne Johansson ◽  
Laura Pozzi ◽  
...  
Keyword(s):  
Raphe Nuclei ◽  
Brain Atlas ◽  
Median Raphe ◽  
Serotonergic Neurons ◽  
Whole Brain ◽  
Raphé Nuclei ◽  
Median Raphé

Development of serotonergic neurons of dorsal raphe nuclei in mice with knockout of monoamine oxidase a and 5-HT1A and 5-HT1B autoreceptor

2009 ◽  
Vol 40 (4) ◽  
pp. 212-221 ◽  
Author(s):  
N. A. Urtikova ◽  
A. Ya. Sapronova ◽  
M. -J. Brisorgueil ◽  
D. Verge ◽  
M. V. Ugryumov
Keyword(s):  
Monoamine Oxidase ◽  
Dorsal Raphe ◽  
Monoamine Oxidase A ◽  
Raphe Nuclei ◽  
Serotonergic Neurons ◽  
Raphé Nuclei ◽  
Dorsal Raphé
Sign in / Sign up

Export Citation Format

Share Document