scholarly journals Interleukin-10 Is Produced by a Specific Subset of Taste Receptor Cells and Critical for Maintaining Structural Integrity of Mouse Taste Buds

2014 ◽  
Vol 34 (7) ◽  
pp. 2689-2701 ◽  
Author(s):  
P. Feng ◽  
J. Chai ◽  
M. Zhou ◽  
N. Simon ◽  
L. Huang ◽  
...  
Keyword(s):  
Structural Integrity ◽  
Taste Receptor ◽  
Interleukin 10 ◽  
Taste Buds ◽  
Receptor Cells ◽  
Specific Subset ◽  
Taste Receptor Cells ◽  
Mouse Taste

Age‐related electrophysiological changes in mouse taste receptor cells

2020 ◽  
Author(s):  
Keita Takeuchi ◽  
Kiyonori Yoshii ◽  
Yoshitaka Ohtubo
Keyword(s):  
Taste Receptor ◽  
Receptor Cells ◽  
Age Related ◽  
Taste Receptor Cells ◽  
Mouse Taste

Umami Changes Intracellular Ca2+Levels Using Intracellular and Extracellular Sources in Mouse Taste Receptor Cells

2006 ◽  
Vol 70 (11) ◽  
pp. 2613-2619 ◽  
Author(s):  
Masataka NARUKAWA ◽  
Tomohiko MORI ◽  
Yukako HAYASHI
Keyword(s):  
Taste Receptor ◽  
Receptor Cells ◽  
Taste Receptor Cells ◽  
Mouse Taste

scholarly journals Estimation of the junctional resistance between electrically coupled receptor cells in Necturus taste buds.

1995 ◽  
Vol 106 (4) ◽  
pp. 705-725 ◽  
Author(s):  
A Bigiani ◽  
S D Roper
Keyword(s):  
Lucifer Yellow ◽  
Electrical Coupling ◽  
Taste Receptor ◽  
Input Resistance ◽  
Taste Buds ◽  
Dye Coupling ◽  
Receptor Cells ◽  
Taste Cells ◽  
Taste Receptor Cells ◽  
Junctional Resistance

Junctional resistance between coupled receptor cells in Necturus taste buds was estimated by modeling the results from single patch pipette voltage clamp studies on lingual slices. The membrane capacitance and input resistance of coupled taste receptor cells were measured to monitor electrical coupling and the results compared with those calculated by a simple model of electrically coupled taste cells. Coupled receptor cells were modeled by two identical receptor cells connected via a junctional resistance. On average, the junctional resistance was approximately 200-300 M omega. This was consistent with the electrophysiological recordings. A junctional resistance of 200-300 M omega is close to the threshold for Lucifer yellow dye-coupling detection (approximately 500 M omega). Therefore, the true extent of coupling in taste buds might be somewhat greater than that predicted from Lucifer yellow dye coupling. Due to the high input resistance of single taste receptor cells (> 1 G omega), a junctional resistance of 200-300 M omega assures a substantial electrical communication between coupled taste cells, suggesting that the electrical activity of coupled cells might be synchronized.

A paracrine signaling role for serotonin in rat taste buds: expression and localization of serotonin receptor subtypes

2004 ◽  
Vol 286 (4) ◽  
pp. R649-R658 ◽  
Author(s):  
Namik Kaya ◽  
Tiansheng Shen ◽  
Shao-gang Lu ◽  
Fang-li Zhao ◽  
Scott Herness
Keyword(s):  
Serotonin Receptor ◽  
Taste Receptor ◽  
Nerve Fibers ◽  
Taste Buds ◽  
Taste Bud ◽  
Receptor Subtype ◽  
Receptor Subtypes ◽  
Double Labeling ◽  
Receptor Cells ◽  
Taste Receptor Cells

Recent advances in peripheral taste physiology now suggest that the classic linear view of information processing within the taste bud is inadequate and that paracrine processing, although undemonstrated, may be an essential feature of peripheral gustatory transduction. Taste receptor cells (TRCs) express multiple neurotransmitters of unknown function that could potentially participate in a paracrine role. Serotonin is expressed in a subset of TRCs with afferent synapses; additionally, TRCs respond physiologically to serotonin. This study explored the expression and cellular localization of serotonin receptor subtypes in TRCs as a possible route of paracrine communication. RT-PCR was performed on RNA extracted from rat posterior taste buds with 14 primer sets representing 5-HT1 through 5-HT7 receptor subtype families. Data suggest that 5-HT1A and 5-HT3 receptors are expressed in taste buds. Immunocytochemistry with a 5-HT1A-specific antibody demonstrated that subsets of TRCs were immunopositive for 5-HT1A. With the use of double-labeling, serotonin- and 5-HT1A-immunopositive cells were observed exclusively in nonoverlapping populations. On the other hand, 5-HT3-immunopositive taste receptor cells were not observed. This observation, combined with other data, suggests 5-HT3 is expressed in postsynaptic neural elements within the bud. We hypothesize that 5-HT release from TRCs activates postsynaptic 5-HT3 receptors on afferent nerve fibers and, via a paracrine route, inhibits neighboring TRCs via 5-HT1A receptors. The role of the 5-HT1A-expressing TRC within the taste bud remains to be explored.

Subtype-dependent postnatal development of taste receptor cells in mouse fungiform taste buds

2012 ◽  
Vol 35 (11) ◽  
pp. 1661-1671 ◽  
Author(s):  
Yoshitaka Ohtubo ◽  
Masafumi Iwamoto ◽  
Kiyonori Yoshii
Keyword(s):  
Postnatal Development ◽  
Taste Receptor ◽  
Taste Buds ◽  
Receptor Cells ◽  
Taste Receptor Cells

Intercellular signaling in Necturus taste buds: chemical excitation of receptor cells elicits responses in basal cells

1992 ◽  
Vol 67 (5) ◽  
pp. 1316-1324 ◽  
Author(s):  
D. A. Ewald ◽  
S. D. Roper
Keyword(s):  
Taste Receptor ◽  
Taste Buds ◽  
Short Latency ◽  
Taste Bud ◽  
Basal Region ◽  
Basal Cells ◽  
Receptor Cells ◽  
Long Latency Responses ◽  
Long Latency ◽  
Taste Receptor Cells

1. Taste cells in intact taste buds in slices of Necturus lingual epithelium were impaled with microelectrodes for intracellular recording. Two types of cells were investigated: taste receptor cells and basal cells. 2. Impaling cells in the apical end of taste buds resulted in intracellular records from taste receptor cells. Applying short pulses (100- to 200-ms duration) of 140 mM KCl solution to the apical pore elicited receptor potentials in the taste receptor cells. 3. Impaling cells in the base of the taste bud resulted in intracellular records from taste receptor cells and basal cells. KCl applied to the taste pore elicited responses in the basal region that varied greatly in both magnitude and time of onset. The latency of these responses (time of onset compared with the onset of the receptor potential) ranged from 0 to hundreds of milliseconds. 4. Impaled cells were identified by injecting Lucifer yellow after recording KCl responses for 21 cells. KCl responses recorded from identified basal cells all had latencies of greater than 75 ms. KCl responses from identified receptor cells all had latencies of less than 75 ms. 5. One explanation for the long latency of KCl responses recorded in basal cells is that the responses represent postsynaptic potentials. In agreement with this interpretation, long-latency responses, but not short-latency responses, were reversibly reduced by the Ca antagonist Cd (1 mM, 10- to 20-min bath exposure). 6. Long-latency responses also differed from short-latency responses in their voltage dependence. Short-latency responses had the same voltage dependence as apically recorded receptor potentials, increasing with hyperpolarization from resting potential with an extrapolated reversal potential near 0 mV. Long-latency responses were much less dependent on voltage in this range. 7. We measured the spread of exogenously applied KCl with potassium-sensitive electrodes. Long-latency responses were not generated by diffusion of applied KCl to the basal region of the taste bud. A small transient increase in extracellular potassium occurred at the base of the taste bud after chemostimulation at the apical pore. This increase was due to depolarization-evoked release of potassium from taste cells and did not cause the long-latency responses in basal cells. 8. We conclude that short-latency (less than 75 ms) responses recorded from cells situated in the bases of taste buds are electrotonically conducted receptor potentials generated at the apical region. Long-latency (greater than 75 ms) responses are consistent with recording postsynaptic responses in basal cells.

Immunoreactivity to Neuronal Growth-Dependent Membrane Glycoprotein Occurs in a Subset of Taste Receptor Cells in Rat Taste Buds

1987 ◽  
Vol 510 (1 Olfaction and) ◽  
pp. 284-286 ◽  
Author(s):  
THOMAS E. FINGER ◽  
HEMA SRIDHAR ◽  
MARY WOMBLE ◽  
VAR L. St. JEOR ◽  
JOHN C. KINNAMON
Keyword(s):  
Taste Receptor ◽  
Taste Buds ◽  
Membrane Glycoprotein ◽  
Neuronal Growth ◽  
Receptor Cells ◽  
Taste Receptor Cells
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