The neuropeptide 26RFa in the human gut and pancreas: potential involvement in glucose homeostasis

Objective Recent studies performed in mice revealed that the neuropeptide 26RFa regulates glucose homeostasis by acting as an incretin and by increasing insulin sensitivity. However, in humans, an association between 26RFa and the regulation of glucose homeostasis is poorly documented. In this study, we have thus investigated in detail the distribution of 26RFa and its receptor, GPR103, in the gut and the pancreas, and determined the response of this peptidergic system to an oral glucose challenge in obese patients. Design and methods Distribution of 26RFa and GPR103 was examined by immunohistochemistry using gut and pancreas tissue sections. Circulating 26RFa was determined using a specific radioimmunoassay in plasma samples collected during an oral glucose tolerance test. Results 26RFa and GPR103 are present all along the gut but are more abundant in the stomach and duodenum. In the stomach, the peptide and its receptor are highly expressed in the gastric glands, whereas in the duodenum, ileum and colon they are present in the enterocytes and the goblet cells. In the pancreatic islets, the 26RFa/GPR103 system is mostly present in the β cells. During an oral glucose tolerance test, plasma 26RFa profile is different between obese patients and healthy volunteers, and we found strong positive correlations between 26RFa blood levels and the BMI, and with various parameters of insulin secretion and insulin resistance. Conclusion The present data suggest an involvement of the 26RFa/GPR103 peptidergic system in the control of human glucose homeostasis.

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1159-P: One-Hour Glucose during Oral Glucose Tolerance Test Predicts Hyperglycemia Relapse in Black Obese Patients with Hyperglycemic Crises

Diabetes ◽

10.2337/db21-1159-p ◽

2021 ◽

Vol 70 (Supplement 1) ◽

pp. 1159-P

Author(s):

RAM JAGANNATHAN ◽

DAWN SMILEY-BYRD ◽

DARKO STEFANOVSKI ◽

GUILLERMO E. UMPIERREZ ◽

PRIYATHAMA VELLANKI

Keyword(s):

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Tolerance Test ◽

Tolerance Test ◽

Oral Glucose ◽

Obese Patients ◽

Oral Glucose Tolerance

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Erratum to: Influence of Telmisartan on Insulin Response after Glucose Loading in Obese Patients with Hypertension: ARB Trial of Hypertension in Obese Patients with Hyperinsulinemia Assessed by Oral Glucose Tolerance Test (ATHLETE)

Advances in Therapy ◽

10.1007/s12325-011-0089-y ◽

2012 ◽

Vol 29 (4) ◽

pp. 383-384

Author(s):

Yutaka Mori ◽

Takaaki Tanaka ◽

Kenichi Matsuura ◽

Junichi Yokoyama ◽

Kazunori Utsunomiya

Keyword(s):

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Tolerance Test ◽

Insulin Response ◽

Tolerance Test ◽

Oral Glucose ◽

Obese Patients ◽

Oral Glucose Tolerance ◽

Glucose Loading

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Categorization of Pancreatic B-Cells Capacity in Overweight and Obese Patients via Oral Glucose Tolerance Test Using K-Means Clustering Method

SSRN Electronic Journal ◽

10.2139/ssrn.3343656 ◽

2019 ◽

Author(s):

Li Li ◽

Qifa Song ◽

Xi Yang

Keyword(s):

B Cells ◽

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Tolerance Test ◽

Tolerance Test ◽

Oral Glucose ◽

Obese Patients ◽

Oral Glucose Tolerance ◽

Clustering Method ◽

Pancreatic B Cells

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212: Patterns of Time-Dependent Insulin Blood Levels During the Oral Glucose Tolerance Test in Korean Subjects

American Journal of Epidemiology ◽

10.1093/aje/161.supplement_1.s53c ◽

2005 ◽

Vol 161 (Supplement_1) ◽

pp. S53-S53

Author(s):

H S Min ◽

Y Ahn ◽

H M Kim ◽

Y J Park ◽

S J Park ◽

...

Keyword(s):

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Tolerance Test ◽

Tolerance Test ◽

Time Dependent ◽

Blood Levels ◽

Oral Glucose ◽

Oral Glucose Tolerance

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Emerging parameters of the insulin and glucose response on the oral glucose tolerance test: Reproducibility and implications for glucose homeostasis in individuals with and without diabetes

Diabetes Research and Clinical Practice ◽

10.1016/j.diabres.2014.04.023 ◽

2014 ◽

Vol 105 (1) ◽

pp. 88-95 ◽

Cited By ~ 27

Author(s):

Caroline Kaercher Kramer ◽

Vladimir Vuksan ◽

Haysook Choi ◽

Bernard Zinman ◽

Ravi Retnakaran

Keyword(s):

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Homeostasis ◽

Glucose Tolerance Test ◽

Tolerance Test ◽

Oral Glucose ◽

Oral Glucose Tolerance ◽

Glucose Response

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GLUCOSE METABOLISM AND INSULIN RESPONSE TO ORAL GLUCOSE TOLERANCE TEST (OGTT) IN PREPUBERTAL PATIENTS WITH TRANSFUSION DEPENDENT Β-THALASSEMIA (TDT): A LONG-TERM RETROSPECTIVE ANALYSIS

Mediterranean Journal of Hematology and Infectious Diseases ◽

10.4084/mjhid.2021.051 ◽

2021 ◽

Vol 13 (1) ◽

pp. e2021051

Author(s):

Vincenzo De Sanctis ◽

Ashraf Soliman ◽

Ploutarchos Tzoulis ◽

Shahina Daar ◽

Salvatore Di Maio ◽

...

Keyword(s):

Glucose Metabolism ◽

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Homeostasis ◽

Glucose Tolerance Test ◽

Insulin Response ◽

Tolerance Test ◽

Oral Glucose ◽

Insulinogenic Index ◽

Oral Glucose Tolerance

Background: Glucose dysregulation (GD), including prediabetes and diabetes mellitus (DM), is a common complication of transfusion dependent β-thalassemia (TDT) patients. The prevalence increases with age and magnitude of iron overload, affecting a significant proportion of patients. The development of GD is frequently asymptomatic and therefore an early diagnosis, according to the international guidelines, requires an annual oral glucose tolerance test (OGTT) in all TDT patients aged ten years or older. Purpose: This retrospective study aims to evaluate the prevalence of GD in a homogenous population of prepubertal TDT patients and to enhance understanding of the pathogenesis and progression of glucose homeostasis in this group of patients. Methods: A selected group of 28 TDT patients was followed for at least 10.3 years (range: 10.3 - 28.10 years) from prepubertal age (mean 11.0 ± standard deviation 1.1 years) to adulthood (28.7 ± 3.7 years). Glucose tolerance and insulin response to OGTT were assessed, and indices of β-cell function, insulin sensitivity and insulin secretion were calculated. Results: At baseline, 18 TDT patients had normal glucose tolerance (NGT) and 10 isolated impaired fasting glycaemia (IFG), according to the American Diabetes Association (ADA) criteria. Compared to 18 prepubertal healthy controls (mean ± SD age: 10.9 ± 1.1 years), the fasting plasma glucose (FPG), basal insulin level and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index were significantly higher in the group of TDT patients (p= 0.001, 0.01 and 0.012, respectively). At the last observation, 7/18 patients (38.8%) with NGT and 9/10 (90%) with IFG at baseline deteriorated; 3 female patients developed type 2 DM (1 from the NGT group and 2 from the IFG group). Compared to adult controls, TDT patients with NGT had a reduced oral disposition index (DI) (p= 0.006), but no significant difference in HOMA-IR and Matsuda index. Conversely, all insulin indices (HOMA-IR, MI and DI) but one [insulinogenic index (IGI)] were statistically different in TDT patients with GD compared to controls. Conclusion: This study shows a spectrum of disturbances in glucose homeostasis among TDT patients and that prepubertal patients with IFG are at higher risk for developing a deterioration of glucose metabolism.

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