scholarly journals Examination of Interchromosomal Interactions in Vegetatively Growing DiploidSchizosaccharomyces pombeCells by Cre/loxP Site-Specific Recombination

Genetics ◽  
2008 ◽  
Vol 178 (1) ◽  
pp. 99-112 ◽  
Author(s):  
Monika Molnar ◽  
Nancy Kleckner
Keyword(s):  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination

Genome editing of different strains of Aureobasidium melanogenum using an efficient Cre/loxp site-specific recombination system

2019 ◽  
Vol 123 (10) ◽  
pp. 723-731 ◽  
Author(s):  
Zhao Zhang ◽  
Yi Lu ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Hong Jiang ◽  
...  
Keyword(s):  
Genome Editing ◽  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
Different Strains ◽  
Aureobasidium Melanogenum ◽  
Site Specific Recombination System

scholarly journals Controlled Cre/loxP Site-Specific Recombination in the Developing Brain in Medaka Fish, Oryzias latipes

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66597 ◽  
Author(s):  
Teruhiro Okuyama ◽  
Yasuko Isoe ◽  
Masahito Hoki ◽  
Yuji Suehiro ◽  
Genki Yamagishi ◽  
...  
Keyword(s):  
Oryzias Latipes ◽  
Loxp Site ◽  
Developing Brain ◽  
Medaka Fish ◽  
Site Specific ◽  
Site Specific Recombination

Transgene Coplacement and High Efficiency Site-Specific Recombination With the Cre/loxP System in Drosophila

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 715-726 ◽  
Author(s):  
Mark L Siegal ◽  
Daniel L Hartl
Keyword(s):  
Germ Cells ◽  
Maternal Effect ◽  
High Efficiency ◽  
Loxp Site ◽  
Position Effects ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Bacteriophage P1 ◽  
Eye Color ◽  
F2 Generation

Abstract Studies of gene function and regulation in transgenic Drosophila are often compromised by the possibility of genomic position effects on gene expression. We have developed a method, called transgene coplacement, in which any two sequences can be positioned at exactly the same site and orientation in the genome. Transgene coplacement makes use of the bacteriophage P1 system of Cre/loxP site-specific recombination, which we have introduced into Drosophila. In the presence of a cre transgene driven by a dual hsp70-Mosl promoter, a white reporter gene flanked by loxP sites is excised with virtually 100% efficiency both in somatic cells and in germ cells. A strong maternal effect, resulting from Cre recombinase present in the oocyte, is observed as white or mosaic eye color in F1 progeny. Excision in germ cells of the F1 yields a strong grand-maternal effect, observed as a highly skewed ratio of eye-color phenotypes in the F2 generation. The excision reactions of Cre/loxP and the related FLP/FRT system are used to create Drosophila lines in which transgenes are at exactly allelic sites in homologous chromosomes.

Phage P1 Cre-loxP site-specific recombination

1985 ◽  
Vol 184 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Kenneth Abremski ◽  
Ronald Hoess
Keyword(s):  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination

Preferential synapsis of loxP sites drives ordered strand exchange in Cre-loxP site-specific recombination

2005 ◽  
Vol 1 (5) ◽  
pp. 275-282 ◽  
Author(s):  
Kaushik Ghosh ◽  
Chi-Kong Lau ◽  
Kushol Gupta ◽  
Gregory D Van Duyne
Keyword(s):  
Loxp Site ◽  
Strand Exchange ◽  
Site Specific ◽  
Site Specific Recombination

Reversible transfection of human melanocytes mediated by Cre/loxP site-specific recombination system and SV40 large T antigen

2007 ◽  
Vol 16 (5) ◽  
pp. 437-444 ◽  
Author(s):  
Wang Ying ◽  
Hao Fei ◽  
Deng Jun ◽  
Yang Xi-chuan ◽  
Zhong Bai-yu ◽  
...  
Keyword(s):  
Loxp Site ◽  
T Antigen ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
Human Melanocytes ◽  
Site Specific Recombination System

Geometry of the DNA Substrates in Cre-loxP Site-Specific Recombination

2000 ◽  
Vol 17 (sup1) ◽  
pp. 141-146 ◽  
Author(s):  
Feng Guo ◽  
Deshmukh N. Gopaul ◽  
Gregory D. Van Duyne
Keyword(s):  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Dna Substrates

Cloning-free genome engineering in Sinorhizobium meliloti advances applications of Cre/loxP site-specific recombination

2016 ◽  
Vol 233 ◽  
pp. 160-170 ◽  
Author(s):  
Johannes Döhlemann ◽  
Meike Brennecke ◽  
Anke Becker
Keyword(s):  
Sinorhizobium Meliloti ◽  
Genome Engineering ◽  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum

Molecules ◽  
2015 ◽  
Vol 20 (6) ◽  
pp. 10110-10121 ◽  
Author(s):  
Hengyi Sun ◽  
Hao Chen ◽  
Xiaonan Zang ◽  
Pan Hou ◽  
Bingbing Zhou ◽  
...  
Keyword(s):  
Loxp Site ◽  
Gene Transformation ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
Site Specific Recombination System
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