scholarly journals Simultaneous and Sequential Integration by Cre/loxP Site-Specific Recombination in Saccharomyces cerevisiae

2018 ◽  
Vol 28 (5) ◽  
pp. 826-830 ◽  
Author(s):  
Ho-Jung Choi ◽  
Yeon-Hee Kim
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid.

1990 ◽  
Vol 172 (2) ◽  
pp. 610-618 ◽  
Author(s):  
H Matsuzaki ◽  
R Nakajima ◽  
J Nishiyama ◽  
H Araki ◽  
Y Oshima
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Chromosome Engineering ◽  
Yeast Plasmid ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
A Site ◽  
Site Specific Recombination System

scholarly journals Deletions extending from a single Ty1 element in Saccharomyces cerevisiae.

1985 ◽  
Vol 5 (12) ◽  
pp. 3451-3457 ◽  
Author(s):  
K M Downs ◽  
G Brennan ◽  
S W Liebman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Reciprocal Translocation ◽  
Restriction Site ◽  
Chromosomal Rearrangements ◽  
Yeast Cells ◽  
Site Specific ◽  
Site Specific Recombination ◽  
The Third ◽  
Deletion Junction

Chromosomal rearrangements associated with one Ty1 element in the iso-1-cytochrome c (CYC1) region of Saccharomyces cerevisiae yeast cells were examined. Most of the rearrangements were deletions of the three linked genes, CYC1, OSM1, and RAD7, and resulted from recombination involving the single Ty1 element and a solo delta in the same orientation. These deletions differed by the number of Ty1 elements (zero, one, or two) remaining after deletion and by restriction site heterogeneities associated with these elements. A single Ty1 element remained at the deletion junction point much more frequently than no Ty1. Apparently the Ty1-associated delta element nearer to the solo delta was involved more often in recombination than the more distal Ty1-associated delta element. The restriction site data implicate gene conversion and suggest that site-specific recombination within the deltas, if occurring, is not the only mechanism of delta-delta recombination. Three other rearrangements bore deletions which began at the end of the Ty1 element and extended into regions not bearing Ty1 or delta sequences. Two of these deletions eliminated 7 kilobases of DNA, although they differed by an associated reciprocal translocation. The third involved a deletion of 14.7 kilobases of DNA associated with an overlapping inversion.

Site-specific recombination in “petite colony” mutants of Saccharomyces cerevisiae

1977 ◽  
Vol 156 (2) ◽  
pp. 163-175 ◽  
Author(s):  
J. Lazowska ◽  
P. P. Slonimski
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Site Specific ◽  
Site Specific Recombination

Genome editing of different strains of Aureobasidium melanogenum using an efficient Cre/loxp site-specific recombination system

2019 ◽  
Vol 123 (10) ◽  
pp. 723-731 ◽  
Author(s):  
Zhao Zhang ◽  
Yi Lu ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Hong Jiang ◽  
...  
Keyword(s):  
Genome Editing ◽  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Recombination System ◽  
Different Strains ◽  
Aureobasidium Melanogenum ◽  
Site Specific Recombination System

scholarly journals Controlled Cre/loxP Site-Specific Recombination in the Developing Brain in Medaka Fish, Oryzias latipes

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66597 ◽  
Author(s):  
Teruhiro Okuyama ◽  
Yasuko Isoe ◽  
Masahito Hoki ◽  
Yuji Suehiro ◽  
Genki Yamagishi ◽  
...  
Keyword(s):  
Oryzias Latipes ◽  
Loxp Site ◽  
Developing Brain ◽  
Medaka Fish ◽  
Site Specific ◽  
Site Specific Recombination

Transgene Coplacement and High Efficiency Site-Specific Recombination With the Cre/loxP System in Drosophila

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 715-726 ◽  
Author(s):  
Mark L Siegal ◽  
Daniel L Hartl
Keyword(s):  
Germ Cells ◽  
Maternal Effect ◽  
High Efficiency ◽  
Loxp Site ◽  
Position Effects ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Bacteriophage P1 ◽  
Eye Color ◽  
F2 Generation

Abstract Studies of gene function and regulation in transgenic Drosophila are often compromised by the possibility of genomic position effects on gene expression. We have developed a method, called transgene coplacement, in which any two sequences can be positioned at exactly the same site and orientation in the genome. Transgene coplacement makes use of the bacteriophage P1 system of Cre/loxP site-specific recombination, which we have introduced into Drosophila. In the presence of a cre transgene driven by a dual hsp70-Mosl promoter, a white reporter gene flanked by loxP sites is excised with virtually 100% efficiency both in somatic cells and in germ cells. A strong maternal effect, resulting from Cre recombinase present in the oocyte, is observed as white or mosaic eye color in F1 progeny. Excision in germ cells of the F1 yields a strong grand-maternal effect, observed as a highly skewed ratio of eye-color phenotypes in the F2 generation. The excision reactions of Cre/loxP and the related FLP/FRT system are used to create Drosophila lines in which transgenes are at exactly allelic sites in homologous chromosomes.

Phage P1 Cre-loxP site-specific recombination

1985 ◽  
Vol 184 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Kenneth Abremski ◽  
Ronald Hoess
Keyword(s):  
Loxp Site ◽  
Site Specific ◽  
Site Specific Recombination

Preferential synapsis of loxP sites drives ordered strand exchange in Cre-loxP site-specific recombination

2005 ◽  
Vol 1 (5) ◽  
pp. 275-282 ◽  
Author(s):  
Kaushik Ghosh ◽  
Chi-Kong Lau ◽  
Kushol Gupta ◽  
Gregory D Van Duyne
Keyword(s):  
Loxp Site ◽  
Strand Exchange ◽  
Site Specific ◽  
Site Specific Recombination
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