Correlation Between Tumor Cell Differentiation and CEA Levels in Patients with Adenocarcinoma of the Rectum

Adenocarcinoma of the rectum is the most common colorectal cancer in Indonesia. This cancer has the highest recurrence after curative surgical therapy with or without adjuvant therapy. With the advancing modern histopathology and molecular biology, the prognosis after therapy can be predicted through surveillance using tumor cell differentiation and carcinoembryonic antigen (CEA). The aim of this study was to analyze the correlation between tumor cell differentiation and serum CEA level in patients with adenocarcinoma of the rectum in Dr. Hasan Sadikin General Hospital Bandung, Indonesia. This was a retrospective observational analytic study conducted from January 2018- January 2019. There were 36 patients involved in this study consisting of 3 patients (8.3%) diagnosed with Stage II, 10 patients (27.7%) with Stage IIIA, 20 patients (55.5%) with stage IIIB, and 3 patients (8.3%) with stage IIIC. On histopathological examination, it was demonstrated that19 patients (52.8%) were well-differentiated, 15 patients (41.7%) were moderately differentiated, and 2 patients (5.6%) were poorly differentiated. The mean CEA level (CI 95%) for well-differentiated patients before surgery was 138.18 (15.99-260.38) ng/ml while the same level for the moderately differentiated patients was 64.34 (34.34-163.02) ng/ml. The mean CEA level for poorly differentiated patients was 1.55 (6.71-9.81) ng / ml. The result of the Kruskal Wallis test showed a p-value of 0.004. There is a strong correlation between the level of tumor cell differentiation and CEA level.

Download Full-text

Anterior Gradient 2 and Mucin 4 Expression Mirrors Tumor Cell Differentiation in Pancreatic Adenocarcinomas, But Aberrant Anterior Gradient 2 Expression Predicts Worse Patient Outcome in Poorly Differentiated Tumors

Pancreas ◽

10.1097/mpa.0b013e3182a63bc3 ◽

2014 ◽

Vol 43 (1) ◽

pp. 75-81 ◽

Cited By ~ 7

Author(s):

Veronika Brychtova ◽

Marketa Hermanova ◽

Petr Karasek ◽

Jiri Lenz ◽

Iveta Selingerova ◽

...

Keyword(s):

Patient Outcome ◽

Cell Differentiation ◽

Tumor Cell ◽

Poorly Differentiated ◽

Tumor Cell Differentiation

Download Full-text

Expression of GRP and Its Receptor in Well-differentiated Colon Cancer Cells Correlates with the Presence of Focal Adhesion Kinase Phosphorylated at Tyrosines 397 and 407

Journal of Histochemistry & Cytochemistry ◽

10.1177/002215540305100807 ◽

2003 ◽

Vol 51 (8) ◽

pp. 1041-1048 ◽

Cited By ~ 35

Author(s):

Kristina A. Matkowskyj ◽

Kristin Keller ◽

Sarah Glover ◽

Lori Kornberg ◽

Roger Tran-Son-Tay ◽

...

Keyword(s):

Colon Cancer ◽

Cell Differentiation ◽

Tumor Cell ◽

Focal Adhesion Kinase ◽

Focal Adhesion ◽

Human Colon ◽

Human Colon Cancer ◽

Colon Cancers ◽

Well Differentiated ◽

Tumor Cell Differentiation

Gastrin-releasing peptide (GRP) and its receptor (GRP-R) are not normally expressed by epithelial cells lining the colon but are aberrantly expressed in cancer, where they act as morphogens and regulate tumor cell differentiation. Studies of colon cancer formation in mice genetically incapable of synthesizing GRP-R suggested that this receptor's morphogenic properties were mediated via focal adhesion kinase (FAK). We therefore set out to determine the presence of both total and phosphorylated forms of FAK in human colon cancer specimens as a function of tumor cell differentiation and GRP/GRP-R co-expression. Ten colon cancers containing 25 regions of distinct differentiation were randomly selected from our GI Cancer Tumor Bank. All specimens were immunohistochemically probed using antibodies recognizing GRP, GRP-R, total FAK, and FAK specifically phos-phorylated at tyrosine (Y) 397, 407, 576, 577, 861, and 925. Antibody-specific chromogen was determined by quantitative immunohistochemistry (IHC) for each region of defined differentiation. Here we confirm that GRP/GRP-R co-expression is a function of differentiation, with highest levels observed in well-differentiated tumor cells. We also show that the amount of total FAK and of FAK phosphorylated at Y397 and Y407 tightly correlates with differentiation and with the amount of GRP/GRP-R co-expression. These findings are consistent with GRP/GRP-R acting as a morphogen by activating FAK, and suggest that this occurs via phosphorylation of this enzyme at two specific tyrosine residues.

Download Full-text

Acetate supplementation restores chromatin accessibility and promotes tumor cell differentiation under hypoxia

Cell Death and Disease ◽

10.1038/s41419-020-2303-9 ◽

2020 ◽

Vol 11 (2) ◽

Cited By ~ 4

Author(s):

Yang Li ◽

Joshua J. Gruber ◽

Ulrike M. Litzenburger ◽

Yiren Zhou ◽

Yu Rebecca Miao ◽

...

Keyword(s):

Cell Differentiation ◽

Tumor Cell ◽

Chromatin Accessibility ◽

Tumor Cell Differentiation

Download Full-text

Pathological grading for predicting metastasis in phaeochromocytoma and paraganglioma

Endocrine Related Cancer ◽

10.1530/erc-13-0494 ◽

2014 ◽

Vol 21 (3) ◽

pp. 405-414 ◽

Cited By ~ 108

Author(s):

Noriko Kimura ◽

Ryoichi Takayanagi ◽

Nae Takizawa ◽

Eiji Itagaki ◽

Takayuki Katabami ◽

...

Keyword(s):

Vascular Invasion ◽

Significant Negative Correlation ◽

Initial Operation ◽

Dehydrogenase Gene ◽

Prediction Of Metastasis ◽

Poorly Differentiated ◽

The Mean ◽

Well Differentiated ◽

Benign Tumours ◽

Subunit B

Phaeochromocytomas (PHEO) and paragangliomas are rare catecholamine-producing tumours. Although 10–30% of these tumours metastasise, histopathological criteria to discriminate malignant from benign tumours have not been established; therefore, reliable histopathological markers predicting metastasis are urgently required. A total of 163 tumours, including 40 metastatic tumours, collected by the Phaeochromocytoma Study Group in Japan (PHEO-J) were analysed using a system called grading system for adrenal phaeochromocytoma and paraganglioma (GAPP). The tumours were scored based on GAPP criteria as follows: histological pattern, cellularity, comedo-type necrosis, capsular/vascular invasion, Ki67 labelling index and catecholamine type. All tumours were scored from 0 to 10 points and were graded as one of the three types: well-differentiated (WD, 0–2 points), moderately differentiated (MD, 3–6 points) and poorly differentiated (PD, 7–10 points). GAPP scores of the non-metastatic and metastatic groups were 2.08±0.17 and 5.33±0.43 (mean±s.e.m., P<0.001) respectively. There was a significant negative correlation between the GAPP score and the interval until metastasis (r=−0.438, P<0.01). The mean number of years until metastasis after the initial operation was 5.5±2.6 years. The study included 111 WD, 35 MD and 17 PD types. The five-year survival of these groups was 100, 66.8 and 22.4% respectively. In addition, negative immunoreactivity for succinate dehydrogenase gene subunit B (SDHB) was observed in 13 (8%) MD or PD tumours and ten of the 13 (77%) had metastases. Our data indicate that a combination of GAPP classification and SDHB immunohistochemistry might be useful for the prediction of metastasis in these tumours.

Download Full-text