scholarly journals Comparative studies on two tests for detection of Avian influenza a virus and Newcastle disease virus in co-infected Guinea fowl (Numida meleagris)

2018 ◽  
Vol 16 (1) ◽  
pp. 7-10
Author(s):  
Iv. Zarkov
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Influenza A Virus ◽  
Newcastle Disease ◽  
Comparative Studies ◽  
Influenza A ◽  
Guinea Fowl ◽  
Numida Meleagris ◽  
Avian Influenza A Virus

Newcastle Disease and Avian Influenza A Virus in Wild Waterfowl in South Africa

2000 ◽  
Vol 44 (3) ◽  
pp. 655 ◽  
Author(s):  
S. Pfitzer ◽  
D. J. Verwoerd ◽  
G. H. Gerdes ◽  
A. E. Labuschagne ◽  
A. Erasmus ◽  
...  
Keyword(s):  
South Africa ◽  
Avian Influenza ◽  
Influenza A Virus ◽  
Newcastle Disease ◽  
Influenza A ◽  
Avian Influenza A Virus ◽  
Wild Waterfowl

scholarly journals Serosurvey for Newcastle Disease and Avian Influenza A Virus Antibodies in Great Cormorants from France

2002 ◽  
Vol 38 (1) ◽  
pp. 169-171 ◽  
Author(s):  
Marc Artois ◽  
Ruth Manvell ◽  
Emmanuelle Fromont ◽  
Jean-Baptiste Schweyer
Keyword(s):  
Avian Influenza ◽  
Influenza A Virus ◽  
Newcastle Disease ◽  
Influenza A ◽  
Avian Influenza A Virus ◽  
Great Cormorants

scholarly journals Influenza viruses in birds: rapid identification by counterimmunoelectrophoresis

1979 ◽  
Vol 9 (1) ◽  
pp. 128-133
Author(s):  
J Lecomte ◽  
L Berthiaume ◽  
A Boudreault
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Influenza A Virus ◽  
Newcastle Disease ◽  
Influenza A ◽  
Large Scale ◽  
Influenza Viruses ◽  
Epidemiological Surveillance ◽  
Influenza A Viruses ◽  
Virus Particles

Counterimmunoelectrophoresis with an antiserum raised in rabbits against the M protein of the avian N virus proved to be particularly useful for large-scale identification of influenza A virus isolates. Of a total of 231 hemagglutinating agents isolated from 1,656 rectal swabs collected from shore and open-country birds, 158 could be identified as influenza A viruses by counterimmunoelectrophoresis, and 75 were serologically related to Newcastle disease virus by hemagglutination inhibition with an antiserum to Newcastle disease virus. Two isolates contained a mixture of influenza A virus and Newcastle disease virus; although the Newcastle disease virus virus particles outnumbered the influenza A virus particles in a ratio of 1,000:1, as seen by electron microscopy, the latter could be readily detected by counterimmunoelectrophoresis. This type of assay appears to be of potential use for epidemiological surveillance of influenza virus isolated from humans and animals. It combines specificity, sensitivity, and simplicity.

scholarly journals Prevalence of infectious bronchitis and Newcastle disease virus among domestic and wild birds in H5N1 outbreaks areas

2011 ◽  
Vol 5 (08) ◽  
pp. 565-570 ◽  
Author(s):  
Zekiba Tarnagda ◽  
Issaka Yougbare ◽  
Adele Kam ◽  
Marc Christian Tahita ◽  
Jean Bosco Ouedraogo
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Burkina Faso ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Influenza A ◽  
Wild Birds ◽  
Western Region ◽  
Highly Pathogenic ◽  
Infectious Bronchitis

Introduction: The first H5N1 outbreak in Burkina Faso was reported to the World Organization for Animal Health on 3 April 2006. This study aimed to determine the prevalence of avian influenza virus, infectious bronchitis virus, and Newcastle disease virus among domestic and wild birds in highly pathogenic avian influenza (HPAI) H5N1 outbreaks areas. Methodology: We collected paired tracheal and cloacal swabs from 283 birds including 278 domestic and five wild birds (three vultures, one sparrowhawk and one Western Grey Plantain-eater) in the Central Region (Ouagadougou) and the Western Region (Bobo-Dioulasso and Sokoroni) of Burkina Faso. Total RNA extracted from samples were subjected to reverse transcription  and resulting cDNA amplified by PCR using specific primers for detection of Avian Influenza Virus (AIV mainly highly pathogenic H5N1), Infectious Bronchitis Virus (IBV), and Newcastle Disease Virus (NDV) for the first time in Burkina Faso. Results and conclusions: Our results show that 13.8% (39/283) samples were reactive for NDV, and the prevalence of IBV was 3.9% (11/283). None of the 283 birds were co-infected by AIV, IBV and/or NDV in our study areas. The prevalence of influenza A virus was 3.2% (95% CI: 0-6.6) with a 1.7% (95% CI: 0-3.2) prevalence of H5N1 being detected. Positive cases of H5N1 virus were found in two out of three vultures in Ouagadougou, and in three out of 203 local chickens in the Western Region. These results confirm the presence of influenza A H5N1 virus, IBV and NDV in domestic and wild birds in Burkina Faso.

scholarly journals Detection of Influenza A virus contamination in Newcastle Disease live virus vaccines and their pathological effects on visceral organs

2019 ◽  
Vol 9 (1) ◽  
pp. 3812-3816
Keyword(s):  
Avian Influenza ◽  
Influenza A Virus ◽  
Virus Vaccine ◽  
Newcastle Disease ◽  
Influenza A ◽  
Rt Pcr ◽  
Live Virus ◽  
Live Virus Vaccine ◽  
Avian Influenza A Virus ◽  
Influenza Outbreaks

To investigate this perception that the NDV live virus vaccine could be the source of Avian Influenza A virus (H9) contamination. Sixteen samples of ND live virus vaccines were purchased from the local market. Prior to use in birds, the samples were tested for Avian Influenza A virus contamination through RT-PCR and used in live birds for any gross pathology and histopathology changes. All the samples were negative against Avian Influenza A virus. Furthermore, these vaccines were also used in broiler and desi chicks at day 5 and day 21 through eye drop and drinking water route respectively. Then, these birds were slaughtered at day 10, 20, 30 and 40 for any gross pathological and histo-pathological changes against Avian Influenza (H9). There were no macroscopic and microscopic lesions observed in visceral organs like trachea, lungs, liver and spleen for Avian Influenza. The results of the study using RT-PCR indicated that the ND live virus vaccine both (local and imported) was free of Avian Influenza A virus (H9). There was a perception among some technical persons that some Avian Influenza outbreaks in the field might be through the source of Newcastle Disease live virus vaccine. This theory regarding contamination of Avian Influenza A virus in Newcastle Disease live virus vaccines found to be wrong on the basis of this study and these commercial vaccines placed in the market are safe to use against Newcastle Disease and are not source of Avian Influenza outbreaks in the field.

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