Detection of Toxoplasma gondii in amniotic fluid using quantitative real-time PCR method

2007 ◽  
Vol 148 (20) ◽  
pp. 935-938 ◽  
Author(s):  
Bálint Nagy ◽  
Levente Lázár ◽  
Gyula Richárd Nagy ◽  
Zoltán Bán ◽  
Zoltán Papp
Keyword(s):  
Toxoplasma Gondii ◽  
Amniotic Fluid ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Method

Bevezetés: A Toxoplasma gondii (T. gondii) által okozott fertőzés általában tünetmentes, vagy csak enyhe panaszokat okoz. A terhesekre azonban veszélyt jelent a parazita. A T. gondiiv al fertőzött anyáról a kórokozó transplacentalisan a magzatot is megfertőzheti és congenitalis toxoplasmosist okozhat, amely súlyos magzati elváltozásokat idézhet elő. A korai diagnózis a kezelés sikerét növeli. A congenitalis toxoplasmosis kimutatható szerológiai és molekuláris biológiai módszerekkel. Cél: A T. gondii kimutatása a kvantitatív valós idejű PCR-módszerrel magzatvízből. Módszerek: A magzatvízből a DNS-t szilika adszorbciós módszerrel izoláltuk. A T. gondii kimutatása 74 mintából történt meg kvantitatív valós idejű PCR-módszerrel. Eredmények: A szerzők 74 magzatvízmintából hat esetben mutatták ki a parazita jelenlétét. Konklúzió: A kvantitatív valós idejű PCR-módszer megnöveli a kimutatás gyorsaságát és érzékenységét, valamint lehetőséget nyújt a kórokozó mennyiségi meghatározására is. Ez új utat nyithat a prognózis megállapítására, valamint a kezelés eredményességének a monitorizálására.

A Quantitative Real-Time PCR Method Using an X-Linked Gene for Sex Typing in Pigs

2012 ◽  
Vol 54 (2) ◽  
pp. 493-496 ◽  
Author(s):  
Maria Ballester ◽  
Anna Castelló ◽  
Yuliaxis Ramayo-Caldas ◽  
Josep M. Folch
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Sex Typing ◽  
Linked Gene ◽  
Pcr Method

Quantitative Detection of Campylobacter jejuni on Fresh Chicken Carcasses by Real-Time PCR

2007 ◽  
Vol 70 (6) ◽  
pp. 1373-1378 ◽  
Author(s):  
ANNA-CLARA RÖNNER ◽  
HANS LINDMARK
Keyword(s):  
Detection Limit ◽  
Real Time ◽  
Dna Extraction ◽  
Campylobacter Jejuni ◽  
Real Time Pcr ◽  
Close Correlation ◽  
Quantitative Detection ◽  
Quantitative Real Time Pcr ◽  
Direct Plating ◽  
Pcr Method

Campylobacter jejuni infection is a significant cause of foodborne gastroenteritis worldwide. Consumption and handling of poultry products is believed to be the primary risk factor for campylobacteriosis. Risk assessments require quantitative data, and C. jejuni is enumerated usually by direct plating, which sometimes allows growth of non-Campylobacter bacteria. The objective of the present study was to develop a quantitative real-time PCR method (q-PCR) for enumerating C. jejuni in chicken rinse without a culturing step. The procedure to obtain the template for the PCR assay involved (i) filtration of 10 ml of chicken rinse, (ii) centrifugation of the sample, and (iii) total DNA extraction from the pellet obtained using a commercial DNA extraction kit. The detection limit of the method was comparable to that for plating 100 μl of chicken rinse on modified charcoal cefoperazone deoxycholate agar, and the detection limit could be further improved 10-fold by concentrating the DNA eluate by ethanol precipitation. A close correlation for spiked chicken rinse was obtained for the results of the quantitative real-time PCR method and direct plating (r = 0.99). The coefficient of correlation for the methods was 0.87 when samples from chicken carcasses on the slaughter line were analyzed, whereas a lower correlation (r = 0.76) was obtained when samples from retail carcasses were analyzed. Greater variation in the proportion of dead and/or viable but not culturable Campylobacter types in the retail samples may explain the decreased correlation between the methods. Overall, the new method is simple and fast and the results obtained are closely correlated with those for direct plating for samples containing a low proportion of dead Campylobacter cells.

A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat

2017 ◽  
Vol 47 (13) ◽  
pp. 875-884 ◽  
Author(s):  
Ignacio Gisbert Algaba ◽  
Manon Geerts ◽  
Malgorzata Jennes ◽  
Wim Coucke ◽  
Marieke Opsteegh ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Real Time Pcr ◽  
Iso 17025 ◽  
Magnetic Capture ◽  
Pcr Method

scholarly journals A quantitative real-time PCR method for absolute telomere length

BioTechniques ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 807-809 ◽  
Author(s):  
Nathan J. O'Callaghan ◽  
Varinderpal S. Dhillon ◽  
Philip Thomas ◽  
Michael Fenech
Keyword(s):  
Telomere Length ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Method

scholarly journals A human fecal contamination score for ranking recreational sites using the HF183/BacR287 quantitative real-time PCR method

2018 ◽  
Vol 128 ◽  
pp. 148-156 ◽  
Author(s):  
Yiping Cao ◽  
Mano Sivaganesan ◽  
Catherine A. Kelty ◽  
Dan Wang ◽  
Alexandria B. Boehm ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Fecal Contamination ◽  
Quantitative Real Time Pcr ◽  
Pcr Method

Early Detection of Toxoplasma gondii Infection in Mongolian Gerbil by Quantitative Real-Time Pcr

10.1645/18-95 ◽  
2019 ◽  
Vol 105 (1) ◽  
pp. 52 ◽  
Author(s):  
Fangwei Dai ◽  
Xunhui Zhuo ◽  
Qingming Kong ◽  
Jiangtao Du ◽  
Haijie Yu ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Early Detection ◽  
Real Time ◽  
Mongolian Gerbil ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Toxoplasma Gondii Infection

Analysis of alternative promoter usage in expression of HSD11B1 including the development of a transcript-specific quantitative real-time PCR method

2011 ◽  
Vol 191 (1-3) ◽  
pp. 104-112 ◽  
Author(s):  
Claudia A. Staab ◽  
Jochen P. Stegk ◽  
Sierk Haenisch ◽  
Elisabeth Neiß ◽  
Katrin Köbsch ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Alternative Promoter ◽  
Quantitative Real Time Pcr ◽  
Pcr Method ◽  
Promoter Usage
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