Experimental Strategy for High Throughput Materials Development

2012 ◽  
Vol 1425 ◽  
Author(s):  
James N. Cawse
Keyword(s):  
High Throughput ◽  
Experimental Program ◽  
Materials Development ◽  
Experimental Strategy ◽  
Statistical Planning ◽  
Robotic Workstation

ABSTRACTSuccessful experimental strategy in the high throughput world requires coordination of the statistical planning of the experimental program with the optimization of the workflow for efficient use of robotic workstation resources.

scholarly journals Developing an Automated Enzyme Immunoassay for High-Throughput Screening of Bovine Serum Antibodies to Neospora Caninum

2003 ◽  
Vol 8 (1) ◽  
pp. 46-50
Author(s):  
John T. Y. Wu ◽  
Sally Dreger ◽  
Eva Y. W. Chow ◽  
Evelyn E. Bowlby ◽  
Lester S. Y. Wong
Keyword(s):  
Enzyme Immunoassay ◽  
High Throughput ◽  
Bovine Serum ◽  
High Throughput Screening ◽  
Neospora Caninum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Predictive Values ◽  
Automated Assay ◽  
Robotic Workstation ◽  
Elisa Data

An enzyme-linked immunosorbent assay (ELISA) for Neospora caninum antibodies was automated with a robotic workstation, the Beckman Coulter Biomek 2000, to screen 200 bovine sera. Comparing these results with manually run ELISA data, a 95.92% agreement (K = 0.9592) between the two assays was obtained. The automated assay was specific and sensitive with excellent positive and negative predictive values. The results were repeatable and reproducible. The automation flexibility was high and the operation complexity was minimal. High-throughput screening (HTS) for bovine antibodies to Neospora caninum was achieved. The assay was developed according to the internationally recognized ISO17025 standard requirements.

scholarly journals Descriptors for High Throughput in Structural Materials Development

2019 ◽  
Vol 8 (4) ◽  
pp. 22 ◽  
Author(s):  
Matthias Steinbacher ◽  
Gabriela Alexe ◽  
Michael Baune ◽  
Ilya Bobrov ◽  
Ingmar Bösing ◽  
...  
Keyword(s):  
High Throughput ◽  
Material Properties ◽  
Functional Materials ◽  
Structural Materials ◽  
Materials Development ◽  
Characterization Techniques ◽  
Classical Characterization ◽  
Short Time ◽  
Characteristic Microstructure ◽  
Intense Process

The development of novel structural materials with increasing mechanical requirements is a very resource-intense process if conventional methods are used. While there are high-throughput methods for the development of functional materials, this is not the case for structural materials. Their mechanical properties are determined by their microstructure, so that increased sample volumes are needed. Furthermore, new short-time characterization techniques are required for individual samples which do not necessarily measure the desired material properties, but descriptors which can later be mapped on material properties. While universal micro-hardness testing is being commonly used, it is limited in its capability to measure sample volumes which contain a characteristic microstructure. We propose to use alternative and fast deformation techniques for spherical micro-samples in combination with classical characterization techniques such as XRD, DSC or micro magnetic methods, which deliver descriptors for the microstructural state.

scholarly journals An Economic Framework to Prioritize Confirmatory Tests after a High-Throughput Screen

2010 ◽  
Vol 15 (6) ◽  
pp. 680-686 ◽  
Author(s):  
S. Joshua Swamidass ◽  
Joshua A. Bittker ◽  
Nicole E. Bodycombe ◽  
Sean P. Ryder ◽  
Paul A. Clemons
Keyword(s):  
High Throughput ◽  
Optimal Solution ◽  
High Throughput Screen ◽  
Experimental Strategy ◽  
Economic Context ◽  
Economic Framework ◽  
False Discovery ◽  
Screening Experiment ◽  
Confirmatory Tests ◽  
Experimental Strategies

How many hits from a high-throughput screen should be sent for confirmatory experiments? Analytical answers to this question are derived from statistics alone and aim to fix, for example, the false discovery rate at a predetermined tolerance. These methods, however, neglect local economic context and consequently lead to irrational experimental strategies. In contrast, the authors argue that this question is essentially economic, not statistical, and is amenable to an economic analysis that admits an optimal solution. This solution, in turn, suggests a novel tool for deciding the number of hits to confirm and the marginal cost of discovery, which meaningfully quantifies the local economic trade-off between true and false positives, yielding an economically optimal experimental strategy. Validated with retrospective simulations and prospective experiments, this strategy identified 157 additional actives that had been erroneously labeled inactive in at least one real-world screening experiment.

High Throughput Techniques for Fire Resistant Materials Development

2009 ◽  
pp. 421-451 ◽  
Author(s):  
Rick Davis ◽  
Richard Lyon ◽  
Michael Takemori ◽  
Naomi Eidelman
Keyword(s):  
High Throughput ◽  
Materials Development

Combinatorial Materials Development using Gradient Arrays. Sphere Covering Lattice Designs: Designs for Efficient use of Experimental Resources.

2003 ◽  
Vol 804 ◽  
Author(s):  
James N. Cawse
Keyword(s):  
High Throughput ◽  
Combinatorial Chemistry ◽  
Sphere Packing ◽  
Materials Development ◽  
Packing And Covering ◽  
Higher Dimensional ◽  
Combinatorial Materials ◽  
Sphere Covering ◽  
The Cost

ABSTRACTGradient arrays are now common tools in combinatorial chemistry for discovery of new leads to commercial materials. Although the cost per sample has dropped markedly with new high throughput methods, efficient use of experimental resources is still important. Examination of gradient arrays from an informational standpoint suggests that designs which use the concepts of sphere packing and covering will be more efficient than simple gradients. This is especially true in higher dimensional systems.

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