Electrochemical Release of Immobilized IgG Protein

In this paper, we present the electrochemically programmed release of immobilized IgG protein molecules that have been attached to gold coated surfaces via a thiol-gold linkage. Fluorescence microscopy has been used to image the release of fluorescently tagged IgGs in phosphate buffered saline. In this technique, the reductive desorption of self-assembled monolayers is employed for the release of proteins, which are immobilized on the surface either by non-covalent or covalent interactions. The voltage applied for the release of proteins is in a range of -1.5V to -60V.