scholarly journals Vacuolation, proliferation and neoplasia in the liver of Boston Harbor winter flounder (Pseudopleuronectes americanus)

1991 ◽  
Author(s):  
Michael John Moore
Keyword(s):  
Winter Flounder ◽  
Boston Harbor ◽  
Pseudopleuronectes Americanus

Ultrastructure of vacuolated cells in the liver of rock sole and winter flounder living in contaminated environments

1990 ◽  
Vol 48 (3) ◽  
pp. 522-523
Author(s):  
Carla Stehr
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Puget Sound ◽  
Light And Electron Microscopy ◽  
Similar Type ◽  
Winter Flounder ◽  
Boston Harbor ◽  
Liver Lesions ◽  
Pseudopleuronectes Americanus ◽  
Vacuolated Cells

Focal and diffuse areas of nonneoplastic vacuolated cells in the liver have been observed with light microscopy in up to 12% of the demersal rock sole (Lepidopsetta bilineata) inhabiting Eagle Harbor, which is a contaminated bay in Puget Sound. A similar type of liver vacuolation has also been observed at higher prevalences in winter flounder (Pseudopleuronectes americanus) inhabiting contaminated areas of Boston Harbor. This paper compares the ultrastructure of vacuolated liver lesions in feral rock sole with that of winter flounder from contaminated areas of Puget Sound and Boston Harbor, respectively.Rock sole from Eagle Harbor, Puget Sound, and winter flounder from Boston Harbor, were collected by bottom trawl from NOAA research vessels. Fish were killed and immediately necropsied. Adjacent pieces of tissue were collected for light and electron microscopy from grossly visible liver lesions or from the center of the liver. Samples collected for histology were fixed in Dietrich's solution, embedded in paraffin and stained with H&E.

Induced Cytochrome P450 in Winter Flounder (Pseudopleuronectes americanus) from Coastal Massachusetts Evaluated by Catalytic Assay and Monoclonal Antibody Probes

1987 ◽  
Vol 44 (7) ◽  
pp. 1270-1277 ◽  
Author(s):  
John J. Stegeman ◽  
Frances Y. Teng ◽  
Elisabeth A. Snowberger
Keyword(s):  
Monoclonal Antibody ◽  
Cytochrome P450 ◽  
Liver Microsomes ◽  
Immunoblot Analysis ◽  
Environmental Chemicals ◽  
Winter Flounder ◽  
Boston Harbor ◽  
Pseudopleuronectes Americanus ◽  
Aryl Hydrocarbon ◽  
Environmental Induction

Levels of hepatic microsomal ethoxyresorufin O-deethylase (EROD) and aryl hydrocarbon hydroxylase (AHH) activities and sensitivity to inhibition by α-naphthoflavone in winter flounder (Pseudopleuronectes americanus) from Deer Island flats in Boston Harbor, off Plymouth Beach, off Nantucket, and at the outer New Bedford Harbor in Buzzards Bay, Massachusetts, suggested induction of cytochrome P450 by environmental chemicals. Levels of activity were higher in fish from Boston Harbor and Plymouth Bay than from Nantucket and Buzzards Bay. In fish from Buzzards Bay the levels of EROD and AHH activities were closely correlated, with some fish there apparently uninduced. Immunoblot analysis of flounder liver microsomes with a monoclonal antibody (1-12-3) against β-naphthofiavone-inducible scup cytochrome P450E revealed a single cross-reacting protein in untreated fish from all four field sites. A similar protein was induced by β-naphthoflavone treatment. Levels of this flounder protein correlated positively with levels of AHH and EROD activity in Buzzards Bay fish, consistent with a conclusion that some fish there were uninduced. The results demonstrate the induction of a P450E counterpart in flounder in Massachusetts waters and indicate its identity as the AHH and EROD catalyst. The results also establish the use of monoclonal anti-P450E in analysis of environmental induction.

The Winter Flounder (Pseudopleuronectes americanus) in Long Pond, Conception Bay, Newfoundland

1971 ◽  
Vol 28 (8) ◽  
pp. 1153-1165 ◽  
Author(s):  
V. S. Kennedy ◽  
D. H. Steele
Keyword(s):  
Food Intake ◽  
Growth Rates ◽  
Plant Material ◽  
Early Growth ◽  
Winter Flounder ◽  
Pseudopleuronectes Americanus ◽  
Food Items ◽  
Fish Remains ◽  
Males And Females ◽  
Conception Bay

Monthly samples of winter flounder taken in Long Pond from November 1962 to October 1963 indicated that the flounder moved into deeper water (7–10 m) during the summer and returned to shallow water (1–2 m) from September to June. These movements corresponded to the end of the spawning season and the ripening of the gonads respectively. Spawning occurred from March until early June, most of it in May and early June. Most males were mature at age 6 and most females at age 7. Fifty percent of the males and females were mature at 21 and 25 cm respectively. The growth rates of the males and females were similar until the age of 8, after which the females apparently outgrew the males. Early growth and fecundity were similar to those reported for other areas. No feeding took place in December or January but the flounder fed in March and continued to feed throughout the summer; food intake decreased in the fall. They were omnivorous and the type of food eaten varied with the locality. Polychaetes, plant material, and molluscs were the most common food items throughout the year. Capelin eggs and fish remains were found only during a few months of the year but were eaten in great quantities.

Sign in / Sign up

Export Citation Format

Share Document