scholarly journals Molecular Response of Pulp Fibroblasts after Stimulation with Pulp Capping Materials

2020 ◽  
Vol 31 (3) ◽  
pp. 244-251
Author(s):  
Karin Cristina da Silva Modena ◽  
Adriana Maria Calvo ◽  
Carla Renata Sipert ◽  
Bella Luna Colombini-Ishikiriama ◽  
Thiago José Dionísio ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Mrna Expression ◽  
Molecular Response ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Pulp Tissue ◽  
Permeable Membrane ◽  
Trypan Blue Exclusion Method ◽  
Nitric Oxide Release

Abstract This in vitro study evaluated cell viability and metabolism, nitric oxide release and production of two chemokines and one cytokine by cultured human dental pulp fibroblasts (HDPF) in contact with two glass ionomer cements (Ketac Molar-KM and Vitrebond-VB), Single Bond (SB) and calcium hydroxide (Dycal-DY). Cultures of HDPF were established by means of an explant technique. The specimens were prepared under sterile conditions and in disks measuring 5 mm x 2 mm obtained from a prefabricated mold and placed on a permeable membrane to avoid direct contact with the cells. Cytotoxicity was assessed by Trypan Blue exclusion method and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide release in cell supernatant was detected by the Griess Method whereas stromal derived factor-1 alpha (SDF-1α or CXCL12), chemokine (C-X-C motif) ligand 8 [Interleukin 8 (IL-8 or CXCL8)] and interleukin-6 (IL-6) were detected by ELISA. RT-qPCR was employed for gene expression analysis. Statistical analyses were performed by One-way ANOVA followed by Tukey’s post hoc test for materials independent of the time, and Two-way ANOVA followed by Bonferroni correction test for the comparisons between materials and experimental time (p<0.05). Cytotoxic tests showed significant differences only for DY. Protein levels and mRNA expression were significantly increased for IL-8 for both periods of time. IL-6 production increased when fibroblasts were stimulated by KM. SDF-1α protein production and mRNA expression were not affected by any of the materials. There was a decrease in nitrate/nitrite levels only for KM. Although DY caused intense cell death and did not stimulate the production of the inflammatory mediators evaluated in this work, it is known that this event seems to be fundamental for the process of repair of the pulp tissue and formation of mineralized barrier. KM and VB increased production of proteins related to the inflammatory process, thus favoring tissue repair. Therefore, although these glass ionomer cements did not lead to large cell death, they should be used with caution.

scholarly journals Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System

2018 ◽  
Vol 29 (5) ◽  
pp. 419-426 ◽  
Author(s):  
Karin Cristina da Silva Modena ◽  
Adriana Maria Calvo ◽  
Carla Renata Sipert ◽  
Thiago José Dionísio ◽  
Maria Fidela de Lima Navarro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Dental Pulp ◽  
Close Contact ◽  
Adhesive System ◽  
Single Bond ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Method ◽  
Nitric Oxide Release

Abstract This study evaluated in vitro cell viability and metabolism, nitric oxide release and production of chemokines by cultured human dental pulp fibroblasts (DPF) under contact with HEMA and Single Bond. Cultures of DPF were established by means of an explant technique. Once plated, cells were kept under contact with increasing concentrations of HEMA (10, 100 and 1000 nM) or Single Bond (SB) [10-fold serially diluted in culture medium (10-4, 10-3 and 10-2 v/v)] and also with polymerized SB components. Cytotoxicity was assessed by Trypan Blue exclusion method and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Nitric oxide release on cell supernatant was detected by Griess Method whereas chemokines (CXCL12 and CXCL8) were detected by ELISA. RT-qPCR was employed for chemokines gene expression analysis. Cytotoxic tests showed significant differences for SB 10-2. None of the tested materials significantly altered NO levels. Protein levels of CXCL12 were significantly decreased only by HEMA. On the other hand, while CXCL12 mRNA remained unaltered, gene expression of CXCL8 had significant decrease with all materials, except for polymerized SB. In conclusion, Single Bond and HEMA at various concentrations, decreased expression and production of molecules involved in inflammatory processes and, therefore, the use of adhesive systems such as pulp capping materials must be viewed with caution due to its large cytotoxic effect when in close contact with the pulp.

Raloxifene enhances nitric oxide release in rat aorta via increasing endothelial nitric oxide mRNA expression

2002 ◽  
Vol 434 (3) ◽  
pp. 141-149 ◽  
Author(s):  
Roshanak Rahimian ◽  
Gregory P Dubé ◽  
Warda Toma ◽  
Nancy Dos Santos ◽  
Bruce M McManus ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Rat Aorta ◽  
Nitric Oxide Release ◽  
Endothelial Nitric Oxide

scholarly journals S100β Induces Neuronal Cell Death Through Nitric Oxide Release from Astrocytes

2002 ◽  
Vol 69 (6) ◽  
pp. 2294-2301 ◽  
Author(s):  
Jingru Hu ◽  
Adriana Ferreira ◽  
Linda J. Van Eldik
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Nitric Oxide Release

Local and Systemic Responses To Dental Composites and Glass Ionomers

1992 ◽  
Vol 6 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Harold R. Stanley
Keyword(s):  
Gold Foil ◽  
Setting Time ◽  
Resin Composites ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Pulp Tissue ◽  
Systemic Complications ◽  
Restorative Materials ◽  
Light Curing ◽  
Systemic Responses

For many years, the dental profession worked mainly with rather inert restorative materials that had a limited contact with vital tissue, and the opportunity for local and systemic complications was minimal. However, conditions have changed in recent years where the two leading non-mercury-containing materials, resin composites and glass-ionomer cements, are chemically active compounds and can have detrimental effects on pulp tissue. With the advent of light-curing techniques with incremental layering, resin component formulae that were formerly found to be quite irritating to the pulp have become less so with the elimination of the need for matrices and pressure for good adaptation to be gained. As experience revealed the deficiencies and dangers of ultraviolet-light-curing techniques, visible-light-curing systems were developed that provided greater depth of cure, a higher degree of polymerization with less shrinkage with incremental layers, and less porosity. When glass-ionomer cements (GICs) were first introduced, with just one acid (polyacrylic), pulpal responses were classified as bland. With the addition of many more acids to enhance certain characteristics and reduce the setting time, GICs have become more irritating, especially when used as luting agents in areas where the remaining dentin thickness is 0.5 mm or less. Gold foil and amalgam are inert and innocuous restorative materials but require pressure for condensation which creates an exaggerated inflammatory response. This presentation emphasizes the pulpal responses and side-effects of these non-mercury-containing restorative materials and how to keep them within an acceptable range of biocompatibility. Despite the lack of any substantial appearance of soft tissue and systemic responses to resin composites and GICs, the results of a survey of recent literature are included.

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