scholarly journals Optimization of a protocol for the micropropagation of pineapple

2002 ◽  
Vol 24 (2) ◽  
pp. 296-300 ◽  
Author(s):  
WELITON ANTONIO BASTOS DE ALMEIDA ◽  
GESSIONEI SILVA SANTANA ◽  
ADRIANA PINHEIRO MARTINELI RODRIGUEZ ◽  
MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA
Keyword(s):  
Liquid Medium ◽  
Shoot Proliferation ◽  
Axillary Buds ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Sterile Water ◽  
Calcium Hypochlorite ◽  
Shoot Bud ◽  
Explant Source ◽  
Growth Room

The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L.) Merrill) cv. Pérola. Changes in benzylaminopurine (BAP) concentration, type of medium (liquid or solidified) and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%), for three minutes, followed by calcium hypochlorite (2%), for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1), 27 ± 2ºC. After eight weeks, cultures were subcultured for multiplication in MS medium. The following treatments were tested: liquid x solidified medium with different BAP concentrations (0.0, 1.5 or 3.0 mg L-1), and the longitudinal cut, or not, of the shoot bud used as explant. The results showed that liquid medium supplemented with BAP at 1.5 mg L-1, associated with the longitudinal sectioning of the shoot bud used as explant presented the best results, maximizing shoot proliferation. On average, the best treatment would allow for an estimated production of 161,080 plantlets by the micropropagation of the axillary buds of one plant with eight slips and ten buds/slips, within a period of eight months.

scholarly journals Effects of Liquid Medium on Rooting and Acclimation of Regenerated Microshoots of Banana (Musa sapientum L.) cv. Sagar

1970 ◽  
Vol 16 (1) ◽  
pp. 11-18 ◽  
Author(s):  
M Atique Akbar ◽  
Shyamal K Roy
Keyword(s):  
Liquid Medium ◽  
High Frequency ◽  
Shoot Proliferation ◽  
Coconut Water ◽  
Axillary Buds ◽  
High Humidity ◽  
Ms Medium ◽  
Sand Soil ◽  
Half Strength ◽  
Number Of Shoots

A regeneration protocol ensuring a high frequency rooting of micro-shoots derived from apical and axillary buds of suckers of banana cv. Sagar was achieved by using liquid medium. When the explants were cultured on MS medium supplemented with 0.5 mg/l each of BA, Kn and NAA, a large number of shoots developed. With the progression of the number of subcultures, shoot proliferation was enhanced. Addition of 10 % coconut water to the medium increased the number of shoots per culture and growth of individual shoots. Shoots rooted well within two weeks, when they were separated individually and sub-cultured in 1.0 mg/l IBA supplemented half strength MS liquid and agar-gelled medium but rooting percentage and their growth were superior in the liquid medium to agar-gelled medium. Rooted plantlets were transferred to small polythene bags containing sterile sand, soil and humus (1 : 2 : 1) and maintained with a high humidity for acclimation. Acclimation and transplantation performances were found to be superior in plants that were rooted in the liquid medium.Key words: Banana, Liquid medium, Regeneration, Acclimation, TransplantationDOI = 10.3329/ptcb.v16i1.1100Plant Tissue Cult. & Biotech. 16(1): 11-18, 2006 (June)

scholarly journals Proliferation-rate Effects of BAP and Kinetin on Banana (Musa spp. AAA Group) ‘Basrai’

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1253-1255 ◽  
Author(s):  
Aish Muhammad ◽  
Hamid Rashid ◽  
Iqbal Hussain ◽  
S.M. Saqlan Naqvi
Keyword(s):  
Liquid Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Height ◽  
Significant Difference ◽  
Rate Effects ◽  
Single Shoot ◽  
Number Of Shoots

The effect of benzylaminopurine (BAP) and kinetin alone and in combination with indole 3-acetic acid (IAA) on shoot proliferation of ‘Basrai’ (Musa spp., AAA group) was investigated. Shoot tips (4–6 mm) were excised from field-grown suckers to initiate the cultures. Concentrations of BAP and kinetin ranged from 0.0 to 8.0 mg·L−1 each on solid or in liquid MS medium. When liquid medium was used, cultures were agitated continuously on an orbital shaker moving at 40 rpm. Three subculture regimes were employed; after each subculture, the number of shoots regenerated from each explant was counted. The results showed that the multiplication rate was significantly (P ≤ 0.05) dependent upon cytokinin type, its concentration, and type of medium used. The maximum number of shoots regenerated from a single shoot tip was achieved in liquid MS medium containing 4.0 mg·L−1 BAP. There was no significant difference between liquid and solid medium when kinetin was used; however, kinetin at 4.0 mg·L−1 or above yielded significant results as compared with the control and lower kinetin concentrations. The results demonstrated that 4.0 mg·L−1 BAP with 1.0 mg·L−1 IAA in liquid medium was best for shoot multiplication and shoot height during micropropagation of ‘Basrai’.

scholarly journals In Vitro Propagation of Apios americana

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1439-1440 ◽  
Author(s):  
E.R.M. Wickremesinhe ◽  
W.J. Blackmon ◽  
B.D. Reynolds
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Axillary Buds ◽  
Ms Medium ◽  
Butanoic Acid ◽  
Apios Americana

Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

scholarly journals Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

2018 ◽  
Vol 12 (2) ◽  
pp. 405-415
Author(s):  
Paulo Mauricio Centenaro Bueno ◽  
Luiz Antonio Biasi ◽  
Mauro Brasil Dias Tofanelli
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Simple Protocol ◽  
Growth Room ◽  
In Vitro Shoot Proliferation

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

scholarly journals SHOOT GROWTH AND PROLIFERATION OF PEACH UNDER VARYING ENVIRONMENTAL REGIMES.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696a-696 ◽  
Author(s):  
Thomas W. Zimmerman ◽  
Ralph Scorza
Keyword(s):  
Shoot Growth ◽  
Shoot Proliferation ◽  
Stage I ◽  
Stage Ii ◽  
Axillary Buds ◽  
Leaf Number ◽  
Ms Medium ◽  
Plastic Foam ◽  
Environmental Regimes ◽  
Basal Salts

This study examined stage I peach shoot growth under various photoperiods in combination with different vessel closures and compared the influence of BA and Thidiazuron (TDZ) on peach shoot growth during stage II. The basal salts were as described by Almehdi and Parfitt (1986) with 1.0 μM BA, 0.02 μM IBA, 2% sucrose, 0.1% gelrite and 0.4% agar. Shoot growth of peach clone B612615, as determined by leaf number after one month, was similar in vessels capped with Kim-Kaps, Kaputs or PM caps. Plastic foam Identi-Plugs resulted in desiccation of the medium and stressed shoots with reduced growth. A 4 h light/2 h dark photoperiod four times a day provided better growth during stage I than a 16 h light/ 8 h dark photoperiod. For stage II, established shoots of Suncrest, Georgia Bell and Evergreen were grown on MS medium supplemented with 0.02 μM IBA in combination with 1.0 or 10 μM BA or 0.1, 1.0 or 10 μM TDZ. TDZ produced excessive callus resulting in minimal shoot proliferation. Shoot proliferation from axillary buds was greatest with 10 μM BA.

scholarly journals The Effects of Growth Regulators on Shoot Propagation and Rooting of Vitis Following in Vitro Axillary Bud and Shoot Apex Culture

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 516C-516
Author(s):  
Handan Büyükdemirci ◽  
Paul E. Read
Keyword(s):  
Shoot Apex ◽  
Shoot Growth ◽  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Axillary Bud ◽  
Ms Medium ◽  
Axillary Bud Culture

Axillary buds of `Valiant' grapevine (Vitis spp.) grown in vitro were transferred onto Murashige and Skoog (MS) medium supplemented with different cytokinin and auxin combinations and concentrations. It was found that culture medium caused statistically important differences in number of nodes, number of fully expanded leaves, number of multiple shoots, number of roots, and length of shoots. MS medium supplemented with 1.0 mg BA/liter in combination with 0.01 mg NAA/L was found to be the best medium for shoot growth and callus production. MS medium supplemented with the combination of 0.5 mg BA/L and 0.01 mg NAA/L was the best medium for explant rooting. The medium containing BA and NAA encouraged better shoot growth than those containing BA alone. When the concentration of BA in the medium was increased, multiple shoot proliferation and teratological structures of explants increased, but the number of small leaves and length of internode decreased. Axillary bud culture led to better shoot growth than was found for shoot apex culture. The presence of leaves positively affected shoot growth from axillary buds. Also placing the axillary buds horizontally onto the medium gave better shoot proliferation and growth than placing them vertically.

scholarly journals In vitro propagation of five Alocasia species

2013 ◽  
Vol 31 (2) ◽  
pp. 210-215 ◽  
Author(s):  
Arvind Bhatt ◽  
Christine Stanly ◽  
Chan Lai Keng
Keyword(s):  
Liquid Medium ◽  
In Vitro Propagation ◽  
Shake Flask ◽  
Shoot Proliferation ◽  
Ms Medium ◽  
Shoot Height ◽  
Shoot Number ◽  
Significant Difference ◽  
Shoot Tip Explants

The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot growth and proliferation for all the tested species. For A. amazonica, A. cuprea, A. robusta and A. longiloba, shake flask system using liquid medium of the same constituents stimulated more shoot proliferation as compared to agar-gelled medium. However, for A. chaii there was no significant difference. All the in vitro plantlets with well developed roots and leaves were successfully acclimatized with more than 90% survival rate.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn

2010 ◽  
Vol 34 (5) ◽  
pp. 789-796 ◽  
Author(s):  
Vanessa Cristina Stein ◽  
Renato Paiva ◽  
Daiane Peixoto Vargas ◽  
Fernanda Pereira Soares ◽  
Eduardo Alves ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Culture Medium ◽  
Cell Development ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Flower Buds ◽  
Embryo Formation ◽  
Transmission Electron ◽  
Explant Source

Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis.

scholarly journals Effect of Sucrose Concentrations and Incubation Periods on in Vitro Rooting of Moris Pineapple (Ananas comosus)

2019 ◽  
Vol 34 (4) ◽  
pp. 230-242
Author(s):  
Abdelhamid M Hamad
Keyword(s):  
Incubation Period ◽  
In Vitro Rooting ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Incubation Periods ◽  
Half Strength

The effect of 6 sucrose concentrations (5, 10, 15, 20, 25, 30 g/l) over 4 incubation periods (30, 45, 60, 75 days) on in vitro rooting of Moris pineapple cultured in liquid half strength MS medium enriched with IBA at 2.0 mg/l was investigated. At all incubation periods, all shoots in medium enriched with sucrose at 5 g/l failed to root, and no roots formed within the first 30 days in medium enriched with sucrose at 10 g/l. After 30 days of incubation, the highest rooting percentage (66 %), tallest plantlets (23 mm tall), highest (3.4 roots) and longest (5.3 mm) root per shoot were obtained in medium enriched with sucrose at 25, 10, 15, 15 g/l respectively, while after 45 days, the highest of all rooting aspects (75 %, 32.3 mm tall, 3.7 roots, 7 mm long), were obtained in medium enriched with sucrose at 15 g/l. After 60 days, the highest rooting percentage (91.7 %) and tallest plantlets (36.7 mm tall) were obtained in medium enriched with sucrose at 20 g/l while highest roots per shoot (3.7 roots) and longest root (10.7 mm) were obtained in medium enriched with sucrose at 15 g/l. After 75 days, all shoots rooted (100 %) in medium enriched with sucrose at 10 and 20 g/l, while sucrose at 25 g/l resulted in tallest plantlets (46.3 mm tall) and at 20 g/l resulted in highest (4.7 roots) and longest roots (27.3 mm). At each incubation period, there was a different optimum sucrose enrichment for different rooting parameters.

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