scholarly journals Effect of two ram sperm capacitating media on acrosome reaction and zona-free hamster oocyte penetration test

2000 ◽  
Vol 37 (3) ◽  
Author(s):  
Silvia FERRARI ◽  
Valquíria Hyppolito BARNABE ◽  
Roberta Mara ZÜGE ◽  
Maria Amélia ZOGNO
Keyword(s):  
Acrosome Reaction ◽  
Penetration Test ◽  
Hamster Oocyte ◽  
Ram Sperm

Involvement of progesterone and estrogen receptors in the ram sperm acrosome reaction

2021 ◽  
Vol 74 ◽  
pp. 106527
Author(s):  
S. Gimeno-Martos ◽  
M. Santorromán-Nuez ◽  
J.A. Cebrián-Pérez ◽  
T. Muiño-Blanco ◽  
R. Pérez-Pé ◽  
...  
Keyword(s):  
Estrogen Receptors ◽  
Acrosome Reaction ◽  
Ram Sperm ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Steroid hormone receptors and direct effects of steroid hormones on ram spermatozoa

Reproduction ◽  
2017 ◽  
Vol 154 (4) ◽  
pp. 469-481 ◽  
Author(s):  
S Gimeno-Martos ◽  
M González-Arto ◽  
A Casao ◽  
M Gallego ◽  
J A Cebrián-Pérez ◽  
...  
Keyword(s):  
Estrogen Receptors ◽  
Steroid Hormones ◽  
Acrosome Reaction ◽  
Hormone Receptors ◽  
Female Genital Tract ◽  
Equatorial Region ◽  
Steroid Hormone Receptors ◽  
Apical Region ◽  
Ram Sperm

This study was based on the assumption that steroid hormones present in the female genital tract may have a rapid effect on ram spermatozoa by interaction with specific surface receptors. We demonstrate the presence of progesterone (PR) and estrogen (ER) receptors in ram spermatozoa, their localization changes duringin vitrocapacitation and the actions of progesterone (P4) and 17β-estradiol (E2) on ram sperm functionality. Immunolocalization assays revealed the presence of PR mainly at the equatorial region of ram spermatozoa. Western blot analyses showed three bands in ram sperm protein extracts of 40–45 kDa, compatible with those reported for PR in the human sperm membrane, and both classical estrogen receptors (66 kDa, ERα and 55 kDa, ERβ). ERα was located in the postacrosomal region of all the spermatozoa and ERβ on the apical region of 63.7% of the cells. The presence of ERβ was correlated with the percentage of non-capacitated spermatozoa evaluated by chlortetracycline staining (R = 0.848,P < 0.001). This significantly decreased afterin vitrocapacitation and nearly disappeared when acrosome reaction was induced. The addition of P4 and E2 beforein vitrocapacitation resulted in a higher (P < 0.001) acrosome-reacted sperm rate compared with the control (13.0%), noticeably greater after 3 h and when added to a high-cAMP medium (37.3% and 47.0% with E2 and P4, respectively). In conclusion, the results of this study demonstrate for the first time that ovine spermatozoa have progesterone and estrogen receptors and that both steroid hormones are related with the induction of the acrosome reaction.

scholarly journals Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?

2020 ◽  
Vol 21 (6) ◽  
pp. 2093
Author(s):  
Sara Miguel-Jiménez ◽  
Melissa Carvajal-Serna ◽  
Silvia Calvo ◽  
Adriana Casao ◽  
José Álvaro Cebrián-Pérez ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Acrosome Reaction ◽  
No Production ◽  
Sperm Capacitation ◽  
Synthase Regulation ◽  
Oxide Synthase ◽  
Nos Isoforms ◽  
Ram Sperm

Nitric oxide (NO·), synthesized from L-arginine by nitric oxide synthase (NOS), is involved in sperm functionality. NOS isoforms have been detected in spermatozoa from different species, and an increment in NOS activity during capacitation has been reported. This work aims to determine the presence and localization of NOS isoforms in ram spermatozoa and analyse their possible changes during in vitro capacitation. Likewise, we investigated the effect of melatonin on the expression and localization of NOS and NO· levels in capacitated ram spermatozoa. Western blot analysis revealed protein bands associated with neuronal NOS (nNOS) and epithelial NOS (eNOS) but not with inducible NOS (iNOS). However, the three isoforms were detected by indirect immunofluorescence (IFI), and their immunotypes varied over in vitro capacitation with cAMP-elevating agents. NO· levels (evaluated by DAF-2-DA/PI staining) increased after in vitro capacitation, and the presence of L-arginine in the capacitating medium raised NO· production and enhanced the acrosome reaction. Incubation in capacitating conditions with a high-cAMP medium with melatonin modified the NOS distribution evaluated by IFI, but no differences in Western blotting were observed. Melatonin did not alter NO· levels in capacitating conditions, so we could infer that its role in ram sperm capacitation would not be mediated through NO· metabolism.

Seasonal dynamics in serum testosterone level, acrosome reaction induction, and osmotic tolerance of ram sperm

2016 ◽  
Vol 169 ◽  
pp. 104-105
Author(s):  
Ejaz Ahamd ◽  
Zahid Naseer ◽  
Melih Aksoy ◽  
Niyazi Küçük ◽  
İlker Serin ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Seasonal Dynamics ◽  
Acrosome Reaction ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Osmotic Tolerance ◽  
Ram Sperm

The role of sperm creatine kinase in the assessment of male fertility

1994 ◽  
Vol 3 (3) ◽  
pp. 179-197 ◽  
Author(s):  
Gabor Huszar
Keyword(s):  
Male Fertility ◽  
Sperm Quality ◽  
Intrauterine Insemination ◽  
Membrane Integrity ◽  
Semen Parameters ◽  
Penetration Test ◽  
Sperm Binding ◽  
Mucus Penetration ◽  
Hamster Oocyte ◽  
Cellular Markers

The lack of reliable methods to assess sperm fertilizing potential has been a long-standing problem for infertile couples and for their physicians. The most widely used tests, the measurements of sperm concentrations, motility, velocity and morphology in the ejaculate, are of limited utility. Indeed, following intrauterine insemination, a treatment that compensates for low motile sperm concentrations, there were no significant differences found in semen parameters among those who did or did not achieve pregnancies. Other available assays probing for selected sperm functions, such as membrane integrity, acrosome enzyme activity, bovine cervical mucus penetration test, zona-free hamster oocyte penetration test and sperm binding to various carbohydrates,10–13 have all failed thus far to consistently predict male fertility. It became increasingly obvious that there was a need to identify cellular markers of sperm quality and fertilizing potential.

Sign in / Sign up

Export Citation Format

Share Document