scholarly journals Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies

2012 ◽  
Vol 78 (1) ◽  
pp. 37-42
Author(s):  
João Daniel Caliman e Gurgel ◽  
Celina Siqueira Barbosa Pereira ◽  
José Humberto Tavares Guerreiro Fregnani ◽  
Fernando de Andrade Quintanilha Ribeiro
2012 ◽  
Vol 69 (4) ◽  
pp. 363-366 ◽  
Author(s):  
Dragoslava Djeric ◽  
Milan Jovanovic ◽  
Ivan Baljosevic ◽  
Srbislav Blazic ◽  
Milanko Milojevic

Introduction. Etiopathogenetically, there are two types of chollesteatomas: congenital, and acquired. Numerous theories in the literature try to explain the nature of the disease, however, the question about cholesteatomas remain still unanswered. The aim of the study was to present a case of external ear canal cholesteatoma (EEC) developed following microsurgery (ventilation tube insertion and mastoidectomy), as well as to point ant possible mechanisms if its development. Case report. A 16-yearold boy presented a 4-month sense of fullness in the ear and otalgia on the left side. A year before, mastoidectomy and posterior atticotomy were performed with ventilation tube placement due to acute purulent mastoiditis. Diagnosis was based on otoscopy examination, audiology and computed tomography (CT) findings. CT showed an obliterative soft-tissue mass completely filled the external ear canal with associated erosion of subjacent the bone. There were squamous epithelial links between the canal cholesteatoma and lateral tympanic membrane surface. They originated from the margins of tympanic membrane incision made for a ventilation tube (VT) insertion. The position of VT was good as well as the aeration of the middle ear cavity. The tympanic membrane was intact and of normal appearance without middle ear extension or mastoid involvement of cholesteatoma. Cholesteatoma and ventilation tube were both removed. The patient recovered without complications and shortly audiology revealed hearing improving. Follow-up 2 years later, however, showed no signs of the disease. Conclusion. There could be more than one potential delicate mechanism of developing EEC in the ear with VT insertion and mastoidectomy. It is necessary to perform routine otologic surveillance in all patients with tubes. Affected ear CT scan is very helpful in showing the extent of cholesteatoma and bony defects, which could not be assessed by otoscopic examination alone.


2001 ◽  
Vol 115 (11) ◽  
pp. 879-880 ◽  
Author(s):  
William B. Hurst

Twenty-two cases of perforated tympanic membrane due to fungal otitis externa were observed over a five-year period.The diagnosis of fungal otitis externa was made on clinical grounds due to the obvious presence of fungal bloom in the external ear canal. Some perforations were noted at the first treatment after the fungal debris had been removed from the external ear canal using a microscope. Other perforations were observed to develop over a few days. Initially, a discrete area of the tympanic membrane appeared white and opaque. As time progressed the white area disintegrated, forming a perforation. Once the otitis externa had resolved most perforations healed spontaneously. Two that were observed to develop during treatment required a myringoplasty. Another one closed significantly but a tiny persistent perforation required cauterization with trichloracetic acid to encourage it to close over completely. The only residual hearing loss was in a case with almost total disintegration of the tympanic membrane requiring a myringoplasty.Treatment of fungal otitis externa for the patients in this series was aural toilet using suction under a microscope and insertion of a gauze wick saturated in a combination of hydrocortisone, clotrimazole, framycetin and gramicidin.


2006 ◽  
Vol 120 (3) ◽  
pp. 247-249 ◽  
Author(s):  
Chao-Hui Yang ◽  
Chih-Ying Su ◽  
Yu-Ching Wei ◽  
Chung-Feng Hwang

Schwannoma arising from the tympanic membrane is a rare neoplasm. This report describes an external ear canal mass obscuring the tympanic membrane. A transcanal approach identified a tumour adhered to the tympanic membrane. The tumour was excised without myringoplasty. Pathology confirmed the diagnosis of schwannoma. Clinical examination revealed no evidence of recurrence during a follow-up period of one year. The possible origins of schwannoma of the tympanic membrane and lesion management are also discussed.


1994 ◽  
Vol 110 (3) ◽  
pp. 310-317 ◽  
Author(s):  
Hiroyuki Sasaki ◽  
Cheng-Chun Huang

The accumulation of keratinizing epithelium in the middle ear cavity is a crucial factor in the pathogenesis of cholesteatoma. We hypothesize that keratinocytes from the skin of the ear canal migrate and hyperprollferate in response to Inflammation in the middle ear cavity to cause accumulation of keratin debris. In the present study, we Investigated the expression of specific cytokeratins (CKs) in the cholesteatoma matrix to determine whether cholesteatoma is a hyperproliferative disease. Cytokeratin expression was examined in cholesteatoma, meatal skin, and tympanic membrane with two monoclonal antibodies, one for both cytokeratins 13 and 16 (antibody K8.12), and another for cytokeratin 13 only (antibody K5–1A3). CK 13 (MW 51 KD) Is a marker of differentiation and CK 16 (MW 48 KD) is a marker of hyperproliferatlon of keratinocytes. The use of immunoblot probes showed that CKs 13 and 16 were present in cholesteatoma. Immunofluorescenf staining showed the presence of CK 16 In the suprabasal layer of cholesteatoma, which was located near the external ear canal. CK 16 was also localized in the suprabasal layer of meatal skin and tympanic membrane. CK 13 was localized in the basal layer of the cholesteatoma, distal to the external ear canal, but not in the meatal skin and tympanic membrane. Taken together, the present data suggest that cholesteatoma is a hyperprollferative disease and that cholesteatoma expresses CK 16 near the external ear canal and transforms to express CK 13 during growth distally.


2006 ◽  
Vol 47 (1) ◽  
pp. 94-98 ◽  
Author(s):  
JEONGMIN LEE ◽  
KIDONG EOM ◽  
YUNSANG SEONG ◽  
HAEOON LEE ◽  
JINHEE PARK ◽  
...  

1995 ◽  
Vol 109 (12) ◽  
pp. 1219-1221 ◽  
Author(s):  
Wedad F. Abadir ◽  
Venice Nakhla ◽  
Philomina Chong

AbstractThe ability of superglue preparations (cyanoacrylate) to bond instantly to skin as well as inanimate objects presents a special problem when instilled into the external ear canal. We present two cases of patients who had it successfully removed under the microscope with the help of acetone BP to debond it from the skin, without any damage to the meatus or the tympanic membrane. The third patient had the superglue removed without the help of acetone but sustained damage to the tympanic membrane. A literature review of similar cases is included.


2007 ◽  
Vol 28 (3) ◽  
pp. 180-183 ◽  
Author(s):  
Giuseppe Magliulo ◽  
Donato Parrotto ◽  
Barbara Sardella ◽  
Carlo della Rocca ◽  
Massimo Re

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