scholarly journals Roles of Renal Cytochrome P450-Dependent Arachidonic Acid Metabolites in Hypertension.

1992 ◽  
Vol 166 (1) ◽  
pp. 93-106 ◽  
Author(s):  
KEN OMATA ◽  
KEISHI ABE ◽  
HSU-LANG SHEU ◽  
KAZUNORI YOSHIDA ◽  
EIKATSU TSUTSUMI ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Cytochrome P450 ◽  
Arachidonic Acid Metabolites ◽  
Acid Metabolites

The Renal Cytochrome P450 System Generates Novel Arachidonic Acid Metabolites

1989 ◽  
pp. 109-129 ◽  
Author(s):  
Michal Schwartzman ◽  
Mairead A. Carroll ◽  
David Sacerdoti ◽  
Nader G. Abraham ◽  
John C. McGiff
Keyword(s):  
Arachidonic Acid ◽  
Cytochrome P450 ◽  
Cytochrome P450 System ◽  
Arachidonic Acid Metabolites ◽  
Acid Metabolites

Role of cytochrome P450–mediated arachidonic acid metabolites in the pathogenesis of cardiac hypertrophy

2013 ◽  
Vol 45 (2) ◽  
pp. 173-195 ◽  
Author(s):  
Abdulaziz M. S. Alsaad ◽  
Beshay N. M. Zordoky ◽  
Mandy M. Y. Tse ◽  
Ayman O. S. El-Kadi
Keyword(s):  
Arachidonic Acid ◽  
Cytochrome P450 ◽  
Cardiac Hypertrophy ◽  
Arachidonic Acid Metabolites ◽  
Acid Metabolites

scholarly journals Modulation of erythropoiesis by novel human bone marrow cytochrome P450- dependent metabolites of arachidonic acid

Blood ◽  
1991 ◽  
Vol 78 (6) ◽  
pp. 1461-1466 ◽  
Author(s):  
NG Abraham ◽  
E Feldman ◽  
JR Falck ◽  
JD Lutton ◽  
ML Schwartzman
Keyword(s):  
Bone Marrow ◽  
Arachidonic Acid ◽  
Cytochrome P450 ◽  
Stromal Cells ◽  
Hematopoietic Cells ◽  
Monooxygenase System ◽  
Dependent Manner ◽  
Aryl Hydrocarbon ◽  
Arachidonic Acid Metabolites ◽  
Acid Metabolites

Abstract In the hematopoietic system the adherent stromal cells produce cytokines necessary for proliferation and differentiation of hematopoietic cells. In the present study, we showed the ability of adherent stromal cells to generate novel metabolites of arachidonic acid via the NADPH-cytochrome P450-dependent monooxygenase system. These metabolites were recovered in the incubation media, suggesting their release from cells. The formation of arachidonic acid metabolites was inhibited by 7-ethoxyresorufin and SKF-525A, but not by indomethacin or BW-755C. By using two-step high-pressure liquid chromatography (HPLC), bone marrow-adherent stromal cells and incubation media showed the presence of metabolites in a peak eluted at 19 to 20 minutes. The isolated HPLC peak was used to measure its effect on colony-forming unit-erythroid (CFU-E) growth and compare it with that of synthetic cytochrome P450 arachidonate metabolites, 19- and 20- hydroxyeicosatetraenoic (HETE) acid. These bone marrow cytochrome P450 arachidonic acid metabolites at picomolar concentration potentiated erythropoietin (Epo)-induced CFU-E growth by fourfold to sixfold. Addition of 19- and 20-HETE to the bone marrow culture resulted in a potentiating effect on CFU-E number in a dose-dependent manner. 20-HETE was much more potent in stimulating CFU-E growth than 19-HETE at a similar concentration of 10(-11) mol/L. The potentiating effect of 20- HETE resulted in a shifting to the left of the dose-response curve to Epo. To substantiate the finding of an active NADPH-dependent cytochrome P450-metabolizing system, we further examined the ability of adherent cells to metabolize exogenous pharmacologic compounds such as benzo(a)pyrene, a substrate for the heme-cytochrome P450 system, aryl hydrocarbon hydroxylase. The adherent stromal cytochrome P450 metabolizes benzo(a)pyrene at comparable levels to blood vessel endothelial cells. These novel observations underscore the importance of adherent stromal cytochrome P450 to metabolize endogenous substrates, including arachidonic acid, to compounds that may interact in a paracrine manner with Epodependent hematopoietic cells.

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