The Detachment of the "Primary Root Caps" in Rice Plants

Shin-ichiro KAWATA; Shigeru SUZUKI; koou YAMAZAKI

doi:10.1626/jcs.48.303

Studies on Root Formation in Certain Cultivated Plants : III. Relation between primary root formation and canal development in crown roots of rice plants

Japanese Journal of Crop Science ◽

10.1626/jcs.24.232 ◽

1956 ◽

Vol 24 (3) ◽

pp. 232-236 ◽

Cited By ~ 2

Author(s):

Sin'ichiro KAWATA

Keyword(s):

Root Formation ◽

Primary Root ◽

Cultivated Plants ◽

Rice Plants ◽

Crown Roots

Download Full-text

OsFPFL4 is Involved in the Root and Flower Development by Affecting Auxin Levels and ROS Accumulation in Rice (Oryza sativa)

Rice ◽

10.1186/s12284-019-0364-0 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Yaomin Guo ◽

Qi Wu ◽

Zizhao Xie ◽

Bo Yu ◽

Rongfeng Zeng ◽

...

Keyword(s):

Flowering Time ◽

Flower Development ◽

Reverse Genetics ◽

Plant Root ◽

Lateral Roots ◽

Primary Root ◽

Auxin Biosynthesis ◽

Wild Type ◽

Rice Plants ◽

Ros Homeostasis

Abstract Background FPF1 (flowering-promoting factor 1) is one of the important family involved in the genetic control of flowering time in plant. Until now, limited knowledge concerning FPF1 family in rice has been understood. Results As a homologue of AtFPF1, FPF1-like protein 4 of rice (OsFPFL4) is expressed in various tissues of plants. The functions of OsFPFL4 in rice were investigated by the reverse genetics approaches. Plants overexpressing OsFPFL4 have shorter primary root, more lateral roots and adventitious roots than wild type; however, RNA interference (RNAi) of OsFPFL4 significantly inhibits the growth of root system, and also delays the flowering time in rice. Interestingly, increased or repressed expression of OsFPFL4 leads to shrunken anthers and abnormal pollen grains. It is well recognized that auxin plays important roles in plant root and flower development, and the root elongation is also regulated by reactive oxygen species (ROS) homeostasis. Here, our results show that rice plants overexpressing OsFPFL4 accumulate more auxin in the shoot and root, whereas RNAi lines have less auxin than wild type. As expected, the transcript levels of genes responsible for auxin biosynthesis and polar transport are altered in these OsFPFL4 transgenic plants. As to ROS, slightly higher ROS levels were detected in overexpression root and inflorescence than the counterparts of wild type; however, the ROS levels were significantly increased in the RNAi lines, due to increased expression of ROS-producers and reduced expression of ROS-scavengers. Conclusion Our results reveal that OsFPFL4 is involved in modulating the root and flower development by affecting auxin and ROS homeostasis in rice plants. OsFPFL4 controls auxin accumulation via affecting auxin biosynthesis and transport, and also modulates ROS homeostasis by balancing ROS producing and scavenging. Thus, auxin-mediated ROS production might play a role in regulating redox status, which controls plant root and flower development.

Download Full-text