Modeling the Effect of Temperature on the Flower Development Rate of Hybrid Impatiens

The effect of average daily temperature (ADT) on flower bud development and subsequent time to flower was investigated on hybrid impatiens (Impatiens ×hybrida) cultivars Compact Electric Orange, Compact Hot Coral, and Compact Orchid Blush. Plants with a visible flower bud measuring 2 mm in width were placed in one of the four greenhouses with temperature setpoints ranging from 16 to 28 °C. Flower bud width was measured every 3 days in each ADT treatment until flowering. The subsequent days to flower (DTF) from the onset of a visible bud decreased from 36 to 27 days as the ADT increased from 17 to 28 °C. The DTF from visible bud varied by <3 days among the three cultivars across all temperatures; therefore, cultivar data were pooled to create a stronger prediction model. A logistic formula was used to predict the remaining DTF as a function of flower bud width and ADT. The model accurately described the effect of bud width and ADT on flowering time within ±3 days for 87% of the actual DTF across all three cultivars. The resulting flower development model provides greenhouse growers with a guide for manipulating temperature to produce flowering plants for specific market dates based on flower bud width measurements.

Determining the Optimum Night Length for Flower Development in a Modern Poinsettia Cultivar

The effect of night length (NL) on the flower development of poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch) ‘Prestige Red’ was evaluated. Flower initiation occurred by subjecting plants to a 14-hour NL for 10 or 17 days, termed short-day (SD) treatments, and then transferring the plants to each of four NL treatments (11, 12, 13, or 14 hours) to observe the effects of NL on flower development. The plants grown continuously with the 14-h NL treatment were the control group. The timing of first color, visible bud, and anthesis were recorded during flower development, and bract and leaf data were collected at anthesis. Leaf number was unaffected by the SD or NL treatments, suggesting that flower initiation occurred during the 10-day SD treatment before the start of NL treatments; thus, the NL treatments only affected flower development. The timing of first color and visible bud were significantly delayed with the 10-day SD × 11-hour NL treatment relative to the 14-hour NL control; however, first color and visible bud were not delayed with the 17-day SD × 11-hour NL treatment. The 11-hour NL treatment resulted in fewer plants reaching anthesis, and these plants had fewer stem bracts and less bract color development compared with the 12-hour, 13-hour, and 14-hour NL treatments. Therefore, an 11-hour NL is suboptimal for flower development; nonetheless, significant development did occur. The 12-hour NL resulted in less color development than the 13-hour and 14-hour NL treatments in the lowest stem bract positions, but the plants had a commercially acceptable appearance. These results demonstrate that minimal differences in flower development occur with NL ≥12 hours, but that optimal development required NL ≥13 hours.

Analyses of MADS-box Genes Suggest HvMADS56 to Regulate Lateral Spikelet Development in Barley

MADS-box transcription factors are crucial regulators of inflorescence and flower development in plants. Therefore, the recent interest in this family has received much attention in plant breeding programs due to their impact on plant development and inflorescence architecture. The aim of this study was to investigate the role of HvMADS-box genes in lateral spikelet development in barley (Hordeum vulgare L.). A set of 30 spike-contrasting barley lines were phenotypically and genotypically investigated under controlled conditions. We detected clear variations in the spike and spikelet development during the developmental stages among the tested lines. The lateral florets in the deficiens and semi-deficiens lines were more reduced than in two-rowed cultivars except cv. Kristina. Interestingly, cv. Kristina, int-h.43 and int-i.39 exhibited the same behavior as def.5, def.6, semi-def.1, semi-def.8 regarding development and showed reduced lateral florets size. In HOR1555, HOR7191 and HOR7041, the lateral florets continued their development, eventually setting seeds. In contrast, lateral florets in two-rowed barley stopped differentiating after the awn primordia stage giving rise to lateral floret sterility. At harvest, the lines tested showed large variation for all central and lateral spikelet-related traits. Phylogenetic analysis showed that more than half of the 108 MADS-box genes identified are highly conserved and are expressed in different barley tissues. Re-sequence analysis of a subset of these genes showed clear polymorphism in either SNPs or in/del. Variation in HvMADS56 correlated with altered lateral spikelet morphology. This suggests that HvMADS56 plays an important role in lateral spikelet development in barley.

Flower meristem maintenance by TILLERS ABSENT 1 is essential for ovule development in rice

ABSTRACT Plant development depends on the activity of pluripotent stem cells in meristems, such as the shoot apical meristem and the flower meristem. In Arabidopsis thaliana, WUSCHEL (WUS) is essential for stem cell homeostasis in meristems and integument differentiation in ovule development. In rice (Oryza sativa), the WUS ortholog TILLERS ABSENT 1 (TAB1) promotes stem cell fate in axillary meristem development, but its function is unrelated to shoot apical meristem maintenance in vegetative development. In this study, we examined the role of TAB1 in flower development. The ovule, which originates directly from the flower meristem, failed to differentiate in tab1 mutants, suggesting that TAB1 is required for ovule formation. Expression of a stem cell marker was completely absent in the flower meristem at the ovule initiation stage, indicating that TAB1 is essential for stem cell maintenance in the ‘final’ flower meristem. The ovule defect in tab1 was partially rescued by floral organ number 2 mutation, which causes overproliferation of stem cells. Collectively, it is likely that TAB1 promotes ovule formation by maintaining stem cells at a later stage of flower development.

Comparative Transcriptome Analysis Identifies Key Regulatory Genes Involved in Anthocyanin Metabolism During Flower Development in Lycoris radiata

Lycoris is used as a garden flower due to the colorful and its special flowers. Floral coloration of Lycoris is a vital trait that is mainly regulated via the anthocyanin biosynthetic pathway. In this study, we performed a comparative transcriptome analysis of Lycoris radiata petals at four different flower development stages. A total of 38,798 differentially expressed genes (DEGs) were identified by RNA sequencing, and the correlation between the expression level of the DEGs and the anthocyanin content was explored. The identified DEGs are significantly categorized into ‘flavonoid biosynthesis,’ ‘phenylpropanoid biosynthesis,’ ‘Tropane, piperidine and pyridine alkaloid biosynthesis,’ ‘terpenoid backbone biosynthesis’ and ‘plant hormone signal transduction’ by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The candidate genes involved in anthocyanin accumulation in L. radiata petals during flower development stages were also identified, which included 56 structural genes (especially LrDFR1 and LrFLS) as well as 27 key transcription factor DEGs (such as C3H, GATA, MYB, and NAC). In addition, a key structural gene namely LrDFR1 of anthocyanin biosynthesis pathway was identified as a hub gene in anthocyanin metabolism network. During flower development stages, the expression level of LrDFR1 was positively correlated with the anthocyanin content. Subcellular localization revealed that LrDFR1 is majorly localized in the nucleus, cytoplasm and cell membrane. Overexpression of LrDFR1 increased the anthocyanin accumulation in tobacco leaves and Lycoris petals, suggesting that LrDFR1 acts as a positively regulator of anthocyanin biosynthesis. Our results provide new insights for elucidating the function of anthocyanins in L. radiata petal coloring during flower development.

The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry

Flower and fruit development are two key steps for plant reproduction. The ABCE model for flower development has been well established in model plant species; however, the functions of ABCE genes in fruit crops are less understood. In this work, we identified an EMS mutant named R27 in woodland strawberry (Fragaria vesca), showing the conversion of petals, stamens, and carpels to sepaloid organs in a semidominant inheritance fashion. Mapping by sequencing revealed that the class E gene homolog FveSEP3 (FvH4_4g23530) possessed the causative mutation in R27 due to a G to E amino acid change in the conserved MADS domain. Additional fvesep3CR mutants generated by CRISPR/Cas9 displayed similar phenotypes to fvesep3-R27. Overexpressing wild-type or mutated FveSEP3 in Arabidopsis suggested that the mutation in R27 might cause a dominant-negative effect. Further analyses indicated that FveSEP3 physically interacted with each of the ABCE proteins in strawberry. Moreover, both R27 and fvesep3CR mutants exhibited parthenocarpic fruit growth and delayed fruit ripening. Transcriptome analysis revealed that both common and specific differentially expressed genes were identified in young fruit at 6–7 days post anthesis (DPA) of fvesep3 and pollinated wild type when compared to unpollinated wild type, especially those in the auxin pathway, a key hormone regulating fruit set in strawberry. Together, we provided compelling evidence that FveSEP3 plays predominant E functions compared to other E gene homologs in flower development and that FveSEP3 represses fruit growth in the absence of pollination and promotes fruit ripening in strawberry.

Study of Cananga odorata (Lam.) Hook. f. & Thoms. Flower Development: Morphological Variations in an Urban Environment

Cananga odorata is a native plant in the Indonesian archipelago. The flowers are often used to produce essential oils with many uses and a distinct fragrance. This study aims to observe each stage of the Cananga odorata flower development. The flowers were obtained from a home garden in Pasar Minggu, South Jakarta, from November 2020 until January 2021. Further observations of the stamen and pistil developments were conducted using Dino-Lite Edge Digital Microscope AM4115 Series. The results show that Cananga odorata flower development can be categorized into bud, display-petal, initial-flowering, full-flowering, end-flowering, and senescence stages. The flowers require 35 days to develop from bud stage to flower senescence. Stamens and pistils also develop primarily during the bud stages and mature after flower anthesis. Flower mutants were also found and may be caused by a mutation in the flower’s homeotic genes. Each different stages of flower development show a different morphological change in the flower perianth and reproductive organs. A discrepancy of flower morphology within each stage, especially those seen during the anthesis stages, might imply a variation in the flower’s internal factors.

TWISTED DWARF1 regulates Arabidopsis stamen development by differential activation of ABCB-mediated auxin transport

Despite clear evidence that a local accumulation of auxin is likewise critical for male fertility, much less is known about the components that regulate auxin-controlled stamen development.In this study, we analyzed physiological and morphological parameters in mutants of key players of ABCB-mediated auxin transport and spatially and temporally dissected their expression on the protein level as well as auxin fluxes in the Arabidopsis stamens. Our analyses revealed that the FKBP42, TWISTED DWARF1 (TWD1), promotes stamen elongation and, to a lesser extent, anther dehiscence, as well as pollen maturation and thus is required for seed development. Most of the described developmental defects in twd1 are shared with the abcb1 abcb19 mutant, which can be attributed to the fact that TWD1 - as a described ABCB chaperon - is a positive regulator of ABCB1 and ABCB19-mediated auxin transport. However, reduced stamen number was dependent on TWD1 but not on investigated ABCBs, suggesting additional actors down-stream of TWD1. We predict an overall housekeeping function for ABCB1 during earlier stages, while ABCB19 seems to be responsible for the key event of rapid elongation at later stages of stamen development. Our data indicate that TWD1 controls stamen development by differential activation of ABCB-mediated auxin transport in the stamen.HighlightBy using a mix of phenotypical and imaging analyses, we here identify and functionally characterize a new master regulator of flower development.

Delineating the genetic regulation of cannabinoid biosynthesis during female flower development in Cannabis sativa

Cannabinoids are predominantly produced in the glandular trichomes on cannabis female flowers. There is little known on how cannabinoid biosynthesis is regulated during female flower development. We aim to understand the rate-limiting step(s) in the cannabinoid biosynthetic pathway. We investigated the transcript levels of cannabinoid biosynthetic genes as well as cannabinoid contents during 7 weeks of female flower development. We demonstrated that the enzymatic steps for producing CBG, which involve genes GPPS, PT and OAC, could be rate limit cannabinoid biosynthesis. Our findings further suggest that cannabinoid synthases, CBDAS and THCAS in a hemp and medical marijuana variety respectively, are not critical for cannabinoid biosynthesis. The cannabinoid biosynthetic genes are generally upregulated during flower maturation, which indicate glandular trichome development. MeJA can potentially increase cannabinoid production. We propose that biweekly application of 100 μM MeJA staring from flower initiation would be efficacious for promoting cannabinoid biosynthesis.