scholarly journals ISSR (Inter Simple Sequence Repeats) as molecular markers to study genetic diversity in tarantulas (Araneae, Mygalomorphae)

2009 ◽  
Vol 37 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Salima Machkour‐M'Rabet ◽  
Yann Hénaut ◽  
Ariane Dor ◽  
Gabriela Pérez-Lachaud ◽  
Céline Pélissier ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Genetic Diversity in <i>Jatropha platyphylla</i> Accessions Based on Morphological Traits and Inter-Simple Sequence Repeats Molecular Markers

2021 ◽  
Vol 12 (11) ◽  
pp. 1658-1672
Author(s):  
Edith Salazar-Villa ◽  
Lilia Alcaraz-Meléndez ◽  
Josefina León-Félix ◽  
José Basilio Heredia ◽  
Federico Soto-Landeros ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Assessment of Genetic Diversity of Rheum species (Endangered Medicinal Herb of Indian Himalayan Region) using Molecular Markers

2021 ◽  
Vol 16 (11) ◽  
pp. 147-154
Author(s):  
Anjali Uniyal ◽  
Akhilesh Kumar ◽  
Sweta Upadhyay ◽  
Vijay Kumar ◽  
Sanjay Gupta
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Fragment Length Polymorphism ◽  
Dna Polymorphisms ◽  
Inter Simple Sequence Repeats ◽  
Pharmaceutical Industries ◽  
Endangered Medicinal Herb ◽  
Identification And Characterization ◽  
Simple Sequence

The Rheum species are important medicinal plants that are facing extinction due to their unplanned development and overexploitation by pharmaceutical industries. DNA polymorphisms are not prone to environmental modifications, thus they are widely used for the identification and characterization of plants. The use of different molecular markers has enabled the researchers for the valuation of genetic variability and diversity in its natural zone of distribution. The conventional approach may take several years to yield this information. For the estimation of molecular and genetic variations in geographical zone of distribution, various molecular markers technique are available like RAPD (Randomly Amplified Polymorphic DNA), RFLP (Restriction fragment length polymorphism), ISSR (Inter-Simple Sequence Repeats), SSR and AFLP. The uses of different molecular markers for the study of genetic diversity have been discussed in the review.

scholarly journals Analysis of genetic diversity and association of seed and mucilage yields with inter simple sequence repeats (ISSR) polymorphism in a collection of Plantgao species

2020 ◽  
Author(s):  
Bagheri Motahareh ◽  
Bahram Heidrai ◽  
Zolfaghar Shahriari ◽  
Ali Dadkhodaie ◽  
Zahra Heidari ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Phylogenetic Network ◽  
Germplasm Conservation ◽  
Inter Simple Sequence Repeats ◽  
Geographical Regions ◽  
The Mean ◽  
Polymorphic Bands ◽  
Simple Sequence ◽  
Issr Primers

AbstractAnalysis of genetic diversity in medicinal plants assists germplasm conservation and selection for use in breeding schemes. The aims of the present study were to assess genetic diversity and differentiation of several Plantago species using Inter Simple Sequence Repeats (ISSR) markers and identify marker-trait associations (MTAs). Thirty-one Plantago accessions belonging to eight species with various mating system and chromosome number were collected from geographical regions of Iran environments. Polymorphism in the DNA of Plantago accessions were analyzed using polymerase chain reaction (PCR) of 25 ISSR primers. The data for number of polymorphic bands were analyzed on the basis of several genetic diversity parameters. The results of gel analysis indicated that the ISSR primers amplified 5 to 21 polymorphic bands with 100 to 3000 bp size. The mean polymorphism was 83.83% and five primers showed 100% polymorphism among Plantago accessions. The Polymorphism Information Content (PIC) for ISSR as a dominant marker ranged from 0.1103 to 0.3829 with the mean 0.2727 in the species tested. Accessions in P. amplexicaulis and P. pysillum species represented the highest Nei’s and Shannon’s genetic diversity whilst the lowest obtained for P. lagopus. Analysis of phylogenetic network generated by the Neighbor-Net Algorithm showed moderate split of the eight species tested and the network depicted moderate conflict. The principal coordinate analysis (PCoA) results showed lower conflict in separation of accessions of the eight species. Fifty-six significant MTAs were detected for the traits tested in Plantago accessions, of which six were shared between three seed and mucilage traits and 24 were common between two traits. The coefficient of determination (R2) for the identified MTAs varied between 32 and 73%. In conclusion, the results of genetic diversity analysis suggested that ISSR marker could efficiently differentiate Plantago species and the information of genetic diversity might assist Plantago improvement and conservation.

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