scholarly journals Performance of a North American Field Population and a Laboratory Colony of the Potato Tuberworm,Phthorimaea operculella, on Foliage of Resistant and Susceptible Potato Clones

2010 ◽  
Vol 10 (80) ◽  
pp. 1-11 ◽  
Author(s):  
Mahmut Doğramaci ◽  
Ward M. Tingey
Keyword(s):  
North American ◽  
Phthorimaea Operculella ◽  
Field Population ◽  
Laboratory Colony ◽  
Potato Tuberworm

scholarly journals Winter is coming: cold hardiness attributes of a field population of the potato tuberworm Phthorimaea operculella

2015 ◽  
Author(s):  
Stefanos S Andreadis ◽  
Yianna Poulia ◽  
Sofia Noukari ◽  
Barbara Aslanidou ◽  
Matilda Savopoulou-Soultani
Keyword(s):  
Cold Hardiness ◽  
Developmental Stages ◽  
Phthorimaea Operculella ◽  
Field Population ◽  
Freezing Injury ◽  
Winter Mortality ◽  
Supercooling Point ◽  
Short Term ◽  
Significant Difference ◽  
Potato Tuberworm

The potato tuberworm, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is a worldwide pest of solanaceous crops especially devastating to potatoes. In the present study we investigated the cold hardiness profile of short-term acclimated and non-acclimated immature and adult stages of a field population of P. operculella. Late instars displayed the lowest mean supercooling point, for both short-term acclimated and non-acclimated individuals, however, no significant differences were observed among developmental stages. Unlike supercooling capacity, acclimation at 5 oC for 5 days enhanced the ability to survive at subzero temperatures after a 2 h exposure. Mean lethal temperature (LTemp50) of all developmental stages (egg, late instar, pupa and adult) decreased after short-term acclimation, however only adults displayed a significant difference among acclimated and non-acclimated individuals concerning their LTemp50 (-11.1 and -8.3 oC, respectively). Generally, pupae were the most cold tolerant developmental stage followed in decreasing order by the eggs and adults, while interestingly late instars were the least ones. Non-freezing injury above the supercooling point was well documented for all developmental stages indicating a pre-freeze mortality and suggesting that P. operculella is considered to be chill tolerant rather than freeze intolerant. Nevertheless, given its high degree of cold hardiness, winter mortality of P. operculella due to low temperatures is not likely to occur and potential pest outbreak can take place following a mild winter.

Susceptibility of Phthorimaea operculella (Zeller) to Insecticides

1996 ◽  
Vol 31 (4) ◽  
pp. 420-426 ◽  
Author(s):  
Celina Llanderal-Cázares ◽  
Angel Lagunes-Tejeda ◽  
José Luis Carrillo-Sánchez ◽  
Carlos Sosa-Moss ◽  
Jorge Vera-Graziano ◽  
...  
Keyword(s):  
Topical Application ◽  
Methyl Parathion ◽  
Wild Population ◽  
Phthorimaea Operculella ◽  
Technical Grade ◽  
Susceptible Population ◽  
Laboratory Colony ◽  
Potato Tuberworm ◽  
Lethal Doses ◽  
Third Instar Larvae

Seven technical grade insecticides were evaluated against a susceptible population and against two field populations of the potato tuberworm Phthorimaea operculella (Zeller) by topical application to third-instar larvae. Using a standard susceptible laboratory colony, we calculated median lethal doses (LD50) of 0.39, 0.059, 0.12, 0.00022, 0.18, 0.010 and 0.0007 μg/larvae for the insecticides DDT, methyl parathion, ethyl parathion, azinphosmethyl, malathion, carbaryl and permethrin, respectively. By comparison, we found that a wild population from Oyameles, Puebla was susceptible to the insecticides while a population from Leon, Guanajuato was susceptible to DDT, methyl parathion, ethyl parathion, malathion and permethrin, but was resistant to azinphosmethyl and carbaryl.

Cold hardiness attributes of a field population of the potato tuberworm, Phthorimaea operculella

2016 ◽  
Vol 160 (2) ◽  
pp. 109-116 ◽  
Author(s):  
Stefanos S. Andreadis ◽  
Yianna Poulia ◽  
Sofia Noukari ◽  
Barbara Aslanidou ◽  
Matilda Savopoulou-Soultani
Keyword(s):  
Cold Hardiness ◽  
Phthorimaea Operculella ◽  
Field Population ◽  
Potato Tuberworm

scholarly journals Winter is coming: cold hardiness attributes of a field population of the potato tuberworm Phthorimaea operculella

2015 ◽  
Author(s):  
Stefanos S Andreadis ◽  
Yianna Poulia ◽  
Sofia Noukari ◽  
Barbara Aslanidou ◽  
Matilda Savopoulou-Soultani
Keyword(s):  
Cold Hardiness ◽  
Developmental Stages ◽  
Phthorimaea Operculella ◽  
Field Population ◽  
Freezing Injury ◽  
Winter Mortality ◽  
Supercooling Point ◽  
Short Term ◽  
Significant Difference ◽  
Potato Tuberworm

The potato tuberworm, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is a worldwide pest of solanaceous crops especially devastating to potatoes. In the present study we investigated the cold hardiness profile of short-term acclimated and non-acclimated immature and adult stages of a field population of P. operculella. Late instars displayed the lowest mean supercooling point, for both short-term acclimated and non-acclimated individuals, however, no significant differences were observed among developmental stages. Unlike supercooling capacity, acclimation at 5 oC for 5 days enhanced the ability to survive at subzero temperatures after a 2 h exposure. Mean lethal temperature (LTemp50) of all developmental stages (egg, late instar, pupa and adult) decreased after short-term acclimation, however only adults displayed a significant difference among acclimated and non-acclimated individuals concerning their LTemp50 (-11.1 and -8.3 oC, respectively). Generally, pupae were the most cold tolerant developmental stage followed in decreasing order by the eggs and adults, while interestingly late instars were the least ones. Non-freezing injury above the supercooling point was well documented for all developmental stages indicating a pre-freeze mortality and suggesting that P. operculella is considered to be chill tolerant rather than freeze intolerant. Nevertheless, given its high degree of cold hardiness, winter mortality of P. operculella due to low temperatures is not likely to occur and potential pest outbreak can take place following a mild winter.

scholarly journals Insecticide Resistance in Anopheles Albitarsis S.S. From a Rice Production Field, With the First Record of Kdr Mutation in This Species 

2021 ◽  
Author(s):  
Taiza Andrade Braga ◽  
Aline Cordeiro Loureiro ◽  
José Bento Pereira Lima ◽  
Ademir J Martins
Keyword(s):  
Insecticide Resistance ◽  
Taxonomic Status ◽  
First Record ◽  
Blood Feeding ◽  
Resistance Mechanisms ◽  
Field Population ◽  
Low Frequencies ◽  
Laboratory Colony ◽  
Field Samples ◽  
Rice Plantation

Abstract Background: Although there is a vast literature concerning insecticide resistance (IR) in Plasmodium vectors from African and Asian continents, similar studies with Neotropical anophelines are scares. Herein we evaluated the IR profile of Anopheles albitarsis s.s. of a laboratory colony and a natural population collected around a rice plantation field. The laboratory colony is original from a collection performed in this same region more than two decades ago. Methods: We collected An. albitarsis females while resting after blood feeding, around rice field plantations in Massaranduba, SC, Brazil. These females laid their eggs in the laboratory, and the larvae were raised in parallel with our lab colony. To be sure about the field samples’ taxonomic status, we amplified and sequenced the mitochondrial COI gene of a sampling of field captured mosquitoes. We performed a simplified knockdown test with larvae exposed to permethrin and deltamethrin and submitted adult females to a WHO like tube test with the pyrethroids permethrin, deltamethrin, and etofenprox, in addition to the organophosphate malathion. A segment of the voltage-gated sodium channel gene (NaV) was amplified and cloned. Based on the observed sequences, we developed a TaqMan genotyping assay for the variation L1014F and calculated the genotypic and allelic frequencies concerning this SNP in the field population.Results: The COI analyses confirmed the taxonomic status of An. albitarsis s.s in laboratory and field samples. The field population was resistant to pyrethroids but not to malathion. We observed the substitutions L1019R, F1020S, and the classical kdr L1014F in the NaV gene. This classical kdr allele was present under low frequencies in the overall field population (2%), although more frequent in pyrethroid-resistant insects.Conclusions: The An. albitarsis s.s. population from Massaranduba was resistant to pyrethroids, likely due to selection pressure exerted by agrochemical pesticides. We registered the classical kdr mutation in a Brazilian Anopheles species for the first time. Further investigations are necessary to disclose additional resistance mechanisms.

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