PHYSIOLOGICAL ACTIONS OF HUMAN FOLLICLE-STIMULATING HORMONE AND ITS β-SUBUNIT IN REPTILES

1977 ◽  
Vol 74 (3) ◽  
pp. 441-447 ◽  
Author(s):  
PAUL LICHT ◽  
ANTONELLA BONA GALLO ◽  
ANNE STOCKELL HARTREE ◽  
RATNA C. SHOWNKEEN
Keyword(s):  
Biological Activity ◽  
Follicle Stimulating Hormone ◽  
Β Subunit ◽  
Binding Assays ◽  
Mammalian Tissues ◽  
Stimulation Of ◽  
Stimulating Hormone

SUMMARY The actions of human follicle-stimulating hormone (hFSH) and its β-subunit were examined in several assays in reptiles, including effects on lizard testicular activity (growth and androgen production) in vivo, and stimulation of androgen production by snake testes and competition for binding of 125I-labelled hFSH in lizards and snakes in vitro. Binding was also examined with mammalian tissues. The hFSH was highly steroidogenic in the snake and lizard; otherwise results were similar to those observed in mammals. In all cases, the potency of the β-subunit was only a few per cent of the intact hormone. The potency of hFSH in vivo compared with NIH-FSH ovine standards was several 100 times greater than in vitro. Results for stimulation of androgen production in vivo closely paralleled those for binding assays in both reptiles and mammals. In contrast to previous results for ovine FSH β-subunit, human FSH β-subunit has little if any FSH biological activity in reptiles.

In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

2017 ◽  
Vol 241 ◽  
pp. 41-49 ◽  
Author(s):  
Pablo J. Sanchís-Benlloch ◽  
Josephine Nocillado ◽  
Claudia Ladisa ◽  
Joseph Aizen ◽  
Adam Miller ◽  
...  
Keyword(s):  
Biological Activity ◽  
Follicle Stimulating Hormone ◽  
Yellowtail Kingfish ◽  
Seriola Lalandi ◽  
Stimulating Hormone

In Vivo Biological Activity of a Recombinant Salmon Follicle-Stimulating Hormone Analog in Prepubertal Male Coho Salmon.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 574-574
Author(s):  
Koichiro Gen ◽  
Jon T. Dickey ◽  
J. Adam Luckenbach ◽  
Yoji Yamamoto ◽  
Olga Mirochnitchenko ◽  
...  
Keyword(s):  
Biological Activity ◽  
Coho Salmon ◽  
Follicle Stimulating Hormone ◽  
Hormone Analog ◽  
Stimulating Hormone

Follicle-stimulating hormone-induced prostaglandin E formation in isolated rat ovarian theca

1983 ◽  
Vol 97 (1) ◽  
pp. 43-49 ◽  
Author(s):  
U. Zor ◽  
B. Strulovici ◽  
R. Braw ◽  
H. R. Lindner ◽  
A. Tsafriri
Keyword(s):  
Cyclic Amp ◽  
Cyclic Gmp ◽  
Follicle Stimulating Hormone ◽  
Fold Increase ◽  
Prostaglandin E ◽  
Β Subunit ◽  
Biochemical Effects ◽  
Isolated Rat ◽  
Stimulating Hormone

The aim of this study was to search for direct biochemical effects of highly purified FSH on isolated ovarian follicular theca in vitro. Granulosa cells (GC; approximately 1 × 105 cells per follicle) were flushed from isolated follicles of pro-oestrous rats. The remaining theca layer and the isolated GC were incubated with highly purified ovine FSH. Prostaglandin E (PGE) accumulation was measured by radioimmunoassay. Follicle-stimulating hormone induced a 15-fold increase in PGE accumulation over the basal level in the follicular theca, the stimulated rate exceeding threefold that observed in the GC fraction derived from the same follicle. Follicle-stimulating hormone caused no significant increase in cyclic AMP level or steroidogenesis in the theca layer, but was active on these parameters in the GC. In contrast, LH increased the accumulation of cyclic AMP, progesterone and testosterone, as well as of PGE, in follicular theca. Exogenous 8-bromo cyclic AMP or cyclic GMP also stimulated PGE production in follicular theca or GC, but FSH was without any effect on the level of endogenous cyclic GMP in GC or follicular theca. Antibodies to FSH prevented the effect of FSH (but not that of LH) on PGE formation by follicular theca and GC, while antibodies to the β-subunit of LH blocked the effect of LH but not of FSH. We conclude that highly purified FSH has a stimulatory effect on PGE formation by the follicular theca.

A COMPARISON OF PREPARATIONS OF HIGHLY PURIFIED HUMAN PITUITARY FOLLICLE-STIMULATING HORMONE: DIFFERENCES IN THE FOLLICLE-STIMULATING HORMONE POTENCIES AS DETERMINED BY IN-VIVO BIOASSAY, IN-VITRO BIOASSAY AND IMMUNOASSAY

1981 ◽  
Vol 91 (2) ◽  
pp. 353-362 ◽  
Author(s):  
P. L. STORRING ◽  
A. A. ZAIDI ◽  
Y. G. MISTRY ◽  
BERIT FRÖYSA ◽  
BRIDGET E. STENNING ◽  
...  
Keyword(s):  
Biological Activity ◽  
Follicle Stimulating Hormone ◽  
In Vitro Bioassay ◽  
Human Pituitary ◽  
International Reference ◽  
Biological Potency ◽  
Reference Preparation ◽  
In Vivo Bioassay

The FSH potencies of 12 preparations of highly purified human pituitary FSH, originating from six different laboratories, were determined by in-vivo and in-vitro bioassays and by immunoassay in terms of the First International Reference Preparation of Human Pituitary Gonadotrophins (FSH and LH) for Bioassay (IRP; coded 69/104). The contamination of these FSH preparations with LH was also determined. Estimates of protein content were based on the absorbance at 280 nm of solutions of the preparations, assuming that A1%1 cm 280 = 10. The FSH potencies varied between different preparations from 827 i.u./mg to 13 100 i.u./mg by in-vivo bioassay; from 2930 to 14 600 i.u./mg by in-vitro bioassay and from 1680 to 5690 i.u./mg by immunoassay. The ratios of in-vivo biological activity relative to in-vitro biological activity and to immunoreactivity respectively varied between preparations from 0·06 to 2·3 and from 0·15 to 4·1, and there was a significant positive correlation between each of these ratios and the in-vivo biological potency of the preparations; such differences could be due to varying degrees of sialylation between preparations. On the other hand the ratios of in-vitro biological activity to immunoreactivity between preparations were fairly constant (approx. 2). The excess biological activity relative to immunoreactivity observed, in terms of the IRP, in all these materials is consistent with recent findings of some immunoreactive FSH in the IRP unassociated with biological activity. These data did not demonstrate any significant advantage, in terms of FSH in-vivo biological potency, from the use of fresh-frozen rather than acetone-dried pituitary glands for the isolation of FSH. Contamination of all these preparations with LH appeared to be less than 3% (w/w), as determined by in-vitro bioassay and by immunoassay. The results of this study are discussed in relation to the selection of material for an international reference preparation for immunoassay and attention is drawn to the value of high in-vivo biological FSH potency as a criterion of the identity of a preparation as well as of its freedom from contaminants without FSH biological activity.

scholarly journals Inhibin B Is a More Potent Suppressor of Rat Follicle-Stimulating Hormone Release than Inhibin A in Vitro and in Vivo

Endocrinology ◽  
2009 ◽  
Vol 150 (10) ◽  
pp. 4784-4793 ◽  
Author(s):  
Yogeshwar Makanji ◽  
Peter D. Temple-Smith ◽  
Kelly L. Walton ◽  
Craig A. Harrison ◽  
David M. Robertson
Keyword(s):  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Hormone Release ◽  
Inhibin A ◽  
Stimulating Hormone
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