Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues

In the present paper, we report on the use of adsorptive transfer stripping technique in connection with chronopotentiometric stripping analysis for metallothionein determination and of differential pulse anodic stripping voltammetry for lead detection in tissues of wild perch (<i>Perca fluviatilis</i>, <i>n</i> = 6) from the Svratka River in Brno, Czech Republic. Primarily, we determined the content of MT in tissues (muscles, gonads, liver and spleen) of perch. We measured the highest content of MT in spleen and liver (100−350 ng MT per gram of fresh weight). We assume that the content of MT determined in perch tissues is probably related with the age of the fish and, therefore, with their exposition to heavy metals naturally occurring in the Svratka River. We detected a lead concentration in the tissues of one perch. It clearly follows from the results that the content of MT well correlates with the concentration of lead.

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Seasonal Variation of Dissolved Lead Speciation in Tagus Estuary, Portugal

Nature Environment and Pollution Technology ◽

10.46488/nept.2021.v20i04.052 ◽

2021 ◽

Vol 20 (4) ◽

Author(s):

M. Praveen Kumar

Keyword(s):

Binding Sites ◽

Lead Concentration ◽

Phytoplankton Bloom ◽

Anodic Stripping Voltammetry ◽

Stripping Voltammetry ◽

Differential Pulse ◽

Quality Parameters ◽

Lead Speciation ◽

Major Cations ◽

Similar Decrease

The behavior of lead species from Tagus estuarine water collected during winter (January), spring (April), and summer (June) seasons were evaluated. Water samples were titrated with Pb+2 followed by differential pulse anodic stripping voltammetry (DPASV). Experimental voltammetric values were interpreted assuming a macromolecular heterogeneous ligand described in a simple way by two types of binding sites, CL1 and CL2, where CL1 is related to stronger binding groups with lower concentration compared to CL2. Water quality parameters like dissolved organic matter (DOC), pH, salinity, temperature, and total lead concentration were measured during the period under study. The results pointed to a higher concentration of CL1 and CL2 sites in April probably due to the phytoplankton bloom. The decrease of KL1 with the increase of salinity from winter to summer may be caused by the increase of major cations (as Ca2+) in solution. The trend of KL2 followed the pH shift in all seasons since an increase of pH favors Pb2+ complexation with CL2 sites. Finally, the decrease of DOC in summer could be responsible for the decrease in the concentration of the different sites in solution from April to June, with a similar decrease of 35±3% for all of them.

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Electrochemical Evaluation of the Organic Matter Content of Edible Sea and Rock Salts Retailed in the Greek Market.

Current Research in Nutrition and Food Science Journal ◽

10.12944/crnfsj.4.special-issue-october.17 ◽

2016 ◽

Vol 4 (Special-Issue-October) ◽

pp. 125-132 ◽

Cited By ~ 1

Author(s):

Aikaterini Sakellari ◽

Marta Plavšić ◽

Sotirios Karavoltsos ◽

Ioannis Diakos ◽

Manos Dassenakis ◽

...

Keyword(s):

Anodic Stripping Voltammetry ◽

Electrochemical Techniques ◽

Organic Matter Content ◽

Chemical Properties ◽

Stripping Voltammetry ◽

Differential Pulse ◽

Matter Content ◽

Copper Binding ◽

Salt Solutions ◽

Catalytically Active

The release of copper-complexing ligands in 14 sea and 10 rock edible salts retailed in the Greek market has been examined. The concentration of ligands (LT) and the copper-binding strength (logKapp) of salt solutions were determined with Differential Pulse Anodic Stripping Voltammetry (DPASV). The tested salt samples were found to have Cu complexing ligands, at concentrations ranging from <0.1 to 61.2 nmol g-1. In none of the salt samples examined (except a single one) were LTvalues exceeded by the corresponding total copper concentrations, indicating that Cu is fully complexed. Aiming to partially characterize the physico-chemical properties of the organic material contained in salts, the surface active substances (SAS) and catalytically active compounds (CAC) were measured, for the first time, in salt solutions by sensitive electrochemical techniques. Higher concentrations of organic carbon and SAS were determined in sea samples compared to rock salts.

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Methylmercury Generation in Seawater by Transmethylation Reactions of Organolead and Organotin Compounds With Inorganic Mercury as Monitored by Multinuclear Magnetic-Resonance and Electrochemical Techniques

Australian Journal of Chemistry ◽

10.1071/ch9861167 ◽

1986 ◽

Vol 39 (8) ◽

pp. 1167 ◽

Cited By ~ 15

Author(s):

GN Howell ◽

MJ Oconnor ◽

AM Bond ◽

HA Hudson ◽

PJ Hanna ◽

...

Keyword(s):

Magnetic Resonance ◽

Anodic Stripping Voltammetry ◽

Electrochemical Techniques ◽

Inorganic Mercury ◽

Stripping Voltammetry ◽

Differential Pulse ◽

Differential Pulse Polarography ◽

Resonance Spectroscopy ◽

Ambient Temperatures ◽

Pulse Polarography

The generation of methylmercury species by reactions between inorganic mercury and methylated forms of lead and tin in seawater and other matrices is described. The reactions were investigated by 119Sn, 199Hg and 207Pb multinuclear Fourier transform magnetic resonance spectroscopy and by differential pulse polarography and anodic stripping voltammetry. Methylmercury chloride is shown to be produced from mixtures of inorganic mercury with methyltin or methyllead species over the 10-2-10-8 M concentration range at ambient temperatures in seawater and artificial seawater.

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