scholarly journals Quantitative analysis of chloramphenicol residues in shrimp muscle tissues by Chemiluminescent enzyme immunoassay

2011 ◽  
Vol 23 (No. 6) ◽  
pp. 251-256
Author(s):  
X. Chuanlai ◽  
P. Cifang ◽  
H. Kai ◽  
J. Zhengyu ◽  
W. Wukang
Keyword(s):  
Enzyme Immunoassay ◽  
Limit Of Detection ◽  
Tween 20 ◽  
Detection Range ◽  
Muscle Tissues ◽  
Physico Chemical ◽  
Interassay Coefficient ◽  
Chemiluminescent Enzyme Immunoassay ◽  
Better Than

A competitive indirect chemiluminescent enzyme immunoassay (ic-CLEIA) has been developed for the determination of chloramphenicol (CAP) residues in shrimp. After the optimisation of four physico-chemical parameters, i.e. incubation time, concentration of Tween-20, concentration of PBS and its pH, the method developed gave a limit of detection of 0.01 ng/ml and a detection range from 0.03 ng/ml to 23.7 ng/ml, with an ED<sub>50</sub> of 0.47 ng/ml. The developed method has been validated on spiked shrimp samples in terms of precision (intra- and interassay coefficient variations of less than 10% and 15%, respectively), and of accuracy (mean recovery from 95% to 123%). All these parameters being better than those of the ELISA method which is widely used to detect chloramphenicol, it may be suggested that the CLEIA method can be used to detect aquatic samples instead of ELISA. &nbsp;

Determination of progesterone receptor by chemiluminescent enzyme immunoassay

2003 ◽  
Vol 23 (1) ◽  
pp. 60-61
Author(s):  
Yin Tiejun ◽  
Gu Meijiao ◽  
Zhou Yikai ◽  
Zheng Wen ◽  
Hu Wei ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

Determination of Free Triiodothyronine by Chemiluminescent Enzyme Immunoassay

2008 ◽  
Vol 36 (5) ◽  
pp. 609-614 ◽  
Author(s):  
Zhen LIN ◽  
Xu WANG ◽  
Shi-Qi REN ◽  
Guo-Nan CHEN ◽  
Zhen-Jia LI ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Free Triiodothyronine ◽  
Chemiluminescent Enzyme Immunoassay

Development of sensitive direct chemiluminescent enzyme immunoassay for the determination of dihydroartemisinin in plasma

2015 ◽  
Vol 407 (25) ◽  
pp. 7823-7830 ◽  
Author(s):  
Laura Zehnacker ◽  
Marie-Claire Nevers ◽  
Véronique Sinou ◽  
Dominique Parzy ◽  
Christophe Créminon ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals A Terbium Sensitized Luminescence Method for the Assay of Flubiprofen in Pharmaceutical Formulations

2014 ◽  
Vol 19 (1) ◽  
pp. 54
Author(s):  
Salma M.Z. Al-Kindy ◽  
Arwa Al-Ghazali ◽  
Fakhr Eldin O. Suliman ◽  
Haidar A.J. Al-Lawati
Keyword(s):  
Limit Of Detection ◽  
Tap Water ◽  
Pharmaceutical Formulations ◽  
Aqueous System ◽  
Tween 20 ◽  
Luminescence Method ◽  
Time Resolved ◽  
Relative Standard ◽  
Excellent Reproducibility

A sensitive time-resolved luminescence method for the determination of flubiprofen (FLP) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of terbium (Tb3+ ) by the formation of a ternary complex with FLP in the presence of 4,7 diphenyl 1,10 phenanthroline (DPP) as co-ligand, and Tween-20 as surfactant. The signal for Tb-FLP-DPP was monitored at λex  = 285 nm and λem  = 552 nm. Optimum conditions for the formation of the complex in an aqueous system were TRIS buffer, pH 8.0, DPP (2.5Å~10−7  M), Tween-20 (0.30%) and 4Å~10-5  mol L-1  of Tb3+  which allowed the determination of 20–1000 ng mL-1  of FLP with a limit of detection (LOD) of 10 ng mL-1 . The relative standard deviations of the method ranged between 0.6 and 1.4% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assays of FLP in pharmaceutical formulations and spiked tap water samples with average recoveries of 87% – 95%. 

scholarly journals Multiresidue Method for the Determination of Residues of 251 Pesticides in Fruits and Vegetables by Gas Chromatography/Mass Spectrometry and Liquid Chromatography with Fluorescence Detection

2000 ◽  
Vol 83 (3) ◽  
pp. 698-713 ◽  
Author(s):  
Julie Fillion ◽  
François Sauvé ◽  
Jennifer Selwyn
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Fruits And Vegetables ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Detection Range

Abstract A method is described for the determination of 251 pesticide and degradation product residues in fruit and vegetable samples. Extraction of the sample with acetonitrile is followed by a saltingout step. Co-extractives are removed by passing a portion of the acetonitrile extract through an octadecyl (C18) solid-phase extraction cleanup cartridge and then, in a second cleanup, through a carbon cartridge coupled to an amino propyl cartridge. Determination is by gas chromatography with mass-selective detection in the selected-ion monitoring mode, and by liquid chromatography with post-column reaction and fluorescence detection for N-methyl carbamates. The method has been used for analysis of various fruits and vegetables, such as apple, banana, cabbage, carrot, cucumber, lettuce, orange, pear, pepper, and pineapple. Limits of detection range between 0.02 and 1.0 mg/kg for most compounds. Over 80% of the compounds have a limit of detection of ≤0.04 mg/kg.

Development of chemiluminescent enzyme immunoassay for the determination of malachite green in seafood

2014 ◽  
Vol 26 (2) ◽  
pp. 204-217 ◽  
Author(s):  
Yan Zhang ◽  
Jin-yi Yang ◽  
Hong-tao Lei ◽  
Hong Wang ◽  
Zhen-lin Xu ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Malachite Green ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Determination of Ivermectin in Salmon Muscle Tissue by Liquid Chromatography with Fluorescence Detection

1998 ◽  
Vol 81 (3) ◽  
pp. 549-553 ◽  
Author(s):  
Heidi S Rupp ◽  
Sherri B Turnipseed ◽  
Calvin C Walker ◽  
José E Roybal ◽  
Austin R Long
Keyword(s):  
Atlantic Salmon ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Average Concentration ◽  
Baseline Noise ◽  
Muscle Tissues ◽  
Relative Standard ◽  
Fluorescent Moiety ◽  
Liquid Chromatographic

abstract A liquid chromatographic method was developed for determination of ivermectin B1a (IVR) extracted from raw fortified and incurred Atlantic salmon muscle tissues. The method was also used to determine fortified doramectin (DOR) in Atlantic salmon. Tissue extract was applied to a C8 solid-phase extraction (SPE) column, followed by a silica SPE column. Residues in the eluate were treated with trifluoroacetic anhydride and methylimidazole to dehydrate the IVR molecule and form an aromatic fluorescent moiety with a trifluoroacetic ester. This product was subsequently treated with ammonium acetate in methanol to cleave the ester and convert the functional group back to a stable alcohol form. The analytes were determined by fluorescence with excitation at 272 nm and emission at 465 nm. A Cis Hypersil column was used for analysis with a mobile phase of acetonitrile-water (90 + 10, v/v) and an oven temperature of 65°C. IVR and DOR were determined at 5 fortification levels (1, 5,10, 20, and 40 ppb). Intra-assay absolute recoveries ranged from 75 to 89% for IVR and from 73 to 85% for DOR. Relative standard deviations (RSDs) were &lt;7% in all cases. The limit of detection (3 x baseline noise) was 0.25 ppb extracted from tissue. Incurred tissues had an average concentration of 32 ppb, with an RSD of 3%.

The Emission Spectrographic Determination of Boron in Silicate Materials

1979 ◽  
Vol 33 (4) ◽  
pp. 404-406 ◽  
Author(s):  
L. S. Dale
Keyword(s):  
Lithium Fluoride ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Coal Ash ◽  
Spectrographic Determination ◽  
Silicate Materials ◽  
Better Than

A procedure is described for the spectrographic determination of boron in silicate materials such as soils and coal ash. The method is based on the use of a lithium fluoride-graphite buffer with germanium as internal standard. A correction for SiO band interference is made. Standardization is facilitated using boron-in-graphite standards. The precision of the method is ±10% and the accuracy is better than 15%. The limit of detection is 10 μg g−1.

scholarly journals Spectrofluorimetric determination of amlodipine in human plasma without derivatization

2012 ◽  
Vol 48 (4) ◽  
pp. 719-725 ◽  
Author(s):  
Yucel Kadioglu ◽  
Murat Ozturk
Keyword(s):  
Channel Blocker ◽  
Limit Of Detection ◽  
Plasma Samples ◽  
Experimental Conditions ◽  
Spectrofluorimetric Method ◽  
Relative Standard ◽  
Limit Of Quantitation ◽  
Spectrofluorimetric Determination ◽  
Better Than

A rapid and sensitive spectrofluorimetric method was developed for the determination of amlodipine (AD), a calcium channel blocker, in the plasma. The type of solvent, the wavelength range, and the range of AD concentration were selected to optimize the experimental conditions. The calibration curves were linear (r² >0.997) in the concentration range of 0.1-12.5 ppm of AD. The limit of quantitation and limit of detection values for the method for plasma samples were 0.1 ppm and 0.07 ppm, respectively. The precision calculated as the relative standard deviation was less than 3.5%, and the accuracy (relative error) was better than 5.5% (n=6). The method developed in this study can be directly and easily applied for the determination of AD in the plasma without derivatization in plasma.

Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milk

2012 ◽  
Vol 4 (8) ◽  
pp. 2550 ◽  
Author(s):  
Marina M. Vdovenko ◽  
Anastasia V. Gribas ◽  
Alexandra V. Vylegzhanina ◽  
Ivan Yu. Sakharov
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay
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